关键词: 缺铁性贫血, 淋巴细胞增殖, 细胞因子, 儿童

Abstract: Objective To evaluate the effect of iron deficiency on lymphocyte proliferation and cytokine secretion in children with iron deficiency anemia (IDA),and to explore the causes of the decline of immune function in children caused by IDA. Methods Totally 20 IDA children were selected in IDA group and 20 healthy children were selected as control group from March 4th to April 28th in 2018. The lymphocyte stimulating index under 10μmol/L,20 μmol/L and 50 μmol/L iron supplementation from IDA group and control group was examined by MTT. The production of INF-γ,IL-2,IL-6 and IL-10 by peripheral blood mononuclear cells (PBMC) under different iron supplementation was examined by ELISA. Results 1) In the control group,SI of B and T cells under iron concentration of 20 μmol/L was significantly higher than that in other iron concentration levels,and was also significantly higher than that under iron concentration of 20 μmol/L in IDA group. Moreover,the SI of IDA children was significantly increased after physiological concentration iron supplementation.2) PBMC from IDA patients showed significantly lower production of INF-γ and IL-2 at all iron concentration levels than PBMC from healthy controls,but INF-γ and IL-2 levels at the concentration of 20 μmol/L were significantly higher than those at the concentration of 10 μmol/L group.3) The secretory levels of IL-6 and IL-10 in IDA group were significantly higher than those in control group at the concentration of 10 μmol/L and 20 μmol/L. Conclusion The changes of T,B lymphocyte proliferation and cytokine secretion in IDA children may be one of the reasons for the imbalance of immune function and weakening immune system

Key words: iron deficiency anemia, lymphocytes proliferation, cytokine, children