关键词: IL-1β, 单核苷酸多态性, 败血症, 足月新生儿

Abstract: Objective To study the association between single nucleotide polymorphisms(SNP) in interleukin-1β(IL-1β) and sepsis in full-term neonates,in order to provide theoretical evidence for the prevention of neonatal sepsis. Methods A total of 50 neonates with positive result of blood culture and diagnosed with sepsis in Kunming Children′s Hospital were selected as sepsis group from January to December 2017,and 50 children with clinical symptoms and negative Results of blood culture were selected in clinical sepsis group.Meanwhile,50 children without infection were in control group.The polymorphisms of IL-1β(rs1143627 and rs1143643) were analyzed using the Sequenom SNP,and the genotypic and allelic frequencies among three groups were compared.The relationship between IL-1β genotypes and sepsis was analyzed by Logistic regression. Results No significant differences were found on genotypic frequencies of IL-1β rs1143627(C/T)(P＞0.05)among sepsis group,clinical sepsis group and control group.But the genotypic frequencies of IL-1β rs1143643(C/T) were significantly different among three groups(χ²=15.94, P<0.05).Moreover,there were no significant differences on the allelic frequencies of the SNPs among the three groups(P>0.05).Logistic regression analysis indicated that gestational age was closely associated with neonatal sepsis(OR=0.6,95%CI:0.418-0.915),while the influences of gender,age,polymorphisms in rs1143627 and rs1143643 genes on neonatal sepsis were not significant(P>0.05). Conclusion Gestational age is the influencing factor of neonatal sepsis.IL-1β polymorphisms(rs1143627 and rs1143643) may not have any association with neonatal sepsis.

Key words: interleukin-1β, single nucleotide polymorphism, sepsis, full-term neonates