早产儿早期外周血γδ-T等淋巴细胞影响因素及其对近期结局预测的研究

doi:10.11852/zgetbjzz2021-1011

中国儿童保健杂志 ›› 2022, Vol. 30 ›› Issue (10): 1118-1122.DOI: 10.11852/zgetbjzz2021-1011

早产儿早期外周血γδ-T等淋巴细胞影响因素及其对近期结局预测的研究

金心韫¹, 张晓丽², 王银娟³, 刘嘉欣³, 王小阳², 徐发林¹

1.郑州大学第三附属医院新生儿科,河南郑州 450052;
2.郑州大学第三附属医院脑损伤重点实验室;
3.郑州大学医学科学院

收稿日期:2021-07-01 修回日期:2021-09-16 发布日期:2022-10-14 出版日期:2022-10-10
通讯作者: 徐发林,E-mail:xufalin72@126.com
作者简介:金心韫(1995-),女,江苏人,硕士研究生,主要研究方向为新生儿疾病。
基金资助:
国家自然科学基金面上项目( 81771418);河南省科技厅科技攻关项目(172102310497)

Influencing factors of γδ-T cells in peripheral blood and its predictive value for outcomes in premature infants

JIN Xin-yun^*, ZHANG Xiao-li, WANG Yin-juan, LIU Jia-xin, WANG Xiao-yang, XU Fa-lin

^*Department of Neonatology, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China

Received:2021-07-01 Revised:2021-09-16 Online:2022-10-10 Published:2022-10-14
Contact: XU Fa-lin,E-mail:xufalin72@126.com

摘要/Abstract

摘要： 目的探讨围生期高危因素对早产儿早期外周血γδ-T等淋巴细胞亚群水平的影响,明确γδ-T细胞对早产儿结局的预测价值。方法选取112例胎龄28~37周的早产儿(包括早期早产儿88例、中晚期早产儿24例)为研究对象,选取同期15例胎龄37~42周的足月儿为对照组,采用流式细胞术检测其生后24 h内外周血γδ-T、CD4+、CD8+、CD3+及总淋巴细胞的比例,分析胎龄及早产高危因素对早期淋巴细胞亚群水平的影响,研究早产儿近期结局与γδ-T等淋巴细胞亚群的关系。结果 1)早期早产儿、中晚期早产儿和足月儿外周血γδ-T细胞水平比较,差异有统计学意义(F=8.855,P＜0.001),且早期早产儿显著低于其他两组(P<0.05);2) 不同早产原因组间各淋巴细胞亚群差异无统计学意义(P＞0.05);3)不同医源性早产原因的4个亚组间γδ-T细胞水平差异有统计学意义(F=3.595,P<0.05),且胎儿生长受限组患儿γδ-T细胞比例降低(P<0.05);4)γδ-T等淋巴细胞亚群分布在早发型感染组与无早发型感染组间差异无统计学意义(P＞0.05);5)矫正胎龄6月龄和12月龄时,早产儿发育正常组、脑瘫/发育落后组和死亡组外周血中γδ-T等淋巴细胞亚群水平差异无统计学意义(P＞0.05)。结论早产儿早期外周血γδ-T细胞主要受胎龄影响,并对胎儿生长受限敏感,与早产儿近期发育水平无显著相关性。

关键词: 早产儿, 胎龄, 淋巴细胞亚群, γδ-T细胞, 围生期因素

Abstract: Objective To investigate the influence of perinatal risk factors on γδ-T lymphocyte subsets in peripheral blood of preterm infants, and to determine the prognostic value of γδ-T cells for outcomes of premature infants. Methods A total of 112 preterm infants with gestational age from 28 to 37 weeks were enrolled in this study (including 88 cases of early preterm infants, 24 cases of middle to late preterm infants). Meanwhile 15 infants with gestational age of 37 to 42 weeks were selected into the control group. The levels of γδ-T, CD4+, CD8+, CD3+ and total lymphocytes in peripheral blood within 24 hours after birth were tested by flow cytometry. The influence of gestational age and risk factors of preterm birth on the lymphocyte subsets levels, as well as the relationship between outcomes of premature infants and γδ-T cells were analyzed. Results 1)There was significantly difference in the level of γδ-T cell in peripheral blood among the early preterm infants,middle to late preterm infants and term infants(F=8.855,P<0.001),and the proportion of γδ-T cells in early preterm infants was significantly lower than that in the other two groups(P＜0.05). 2) There was no significant difference in lymphocyte subsets among different causes of preterm birth groups (P＞0.05). 3)The levels of γδ-T cells in the four different iatrogenic preterm delivery subgroups were significantly different(F=3.595,P<0.05), andthe proportion of γδ-T cells in the fetal growth restriction group was significantly decreased (P<0.05). 4) There was no significant difference in the proportion of lymphocyte subsets between early-onset infection group and non-early-onset infection group (P>0.05). 5) No significant difference in γδ-T lymphocyte subsets was found among the normal group, the cerebral palsy/developmental delays group and the death group with 6 and 12 months of corrected age (P>0.05). Conclusion γδ-T cells in early peripheral blood of preterm infants are mainly affected by gestational age and are sensitive to fetal growth restriction, but have no correlation with the short-term development level of preterm infants.

Key words: preterm infants, gestational age, lymphocyte subsets, γδ-T cell, perinatal factors

中图分类号:

R722.6

金心韫, 张晓丽, 王银娟, 刘嘉欣, 王小阳, 徐发林. 早产儿早期外周血γδ-T等淋巴细胞影响因素及其对近期结局预测的研究[J]. 中国儿童保健杂志, 2022, 30(10): 1118-1122.

JIN Xin-yun, ZHANG Xiao-li, WANG Yin-juan, LIU Jia-xin, WANG Xiao-yang, XU Fa-lin. Influencing factors of γδ-T cells in peripheral blood and its predictive value for outcomes in premature infants[J]. Chinese Journal of Child Health Care, 2022, 30(10): 1118-1122.

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早产儿早期外周血γδ-T等淋巴细胞影响因素及其对近期结局预测的研究

Influencing factors of γδ-T cells in peripheral blood and its predictive value for outcomes in premature infants

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