孤独症谱系障碍儿童肠道菌群与症状严重程度相关性研究

doi:10.11852/zgetbjzz2022-1252

中国儿童保健杂志 ›› 2023, Vol. 31 ›› Issue (5): 497-501.DOI: 10.11852/zgetbjzz2022-1252

孤独症谱系障碍儿童肠道菌群与症状严重程度相关性研究

李德欣¹, 陈强¹, 庄志成¹, 陈红¹, 杨秉娇¹, 姜顺香², 周翔¹

1.珠海市妇幼保健院儿童心理卫生科,广东珠海 519001;
2.华大精准营养(深圳)科技有限公司

收稿日期:2022-10-13 修回日期:2023-02-07 发布日期:2023-05-06 出版日期:2023-05-10
通讯作者: 周翔,E-mail:zhouxiangp@163.com
作者简介:李德欣(1994-),女,哈尔滨人,医师,硕士学位,主要研究方向为孤独症谱系障碍疾病。
基金资助:
广东省医学科学技术研究基金项目(A2020580)

Correlation between gutmicrobiota and symptom severity in children with autism spectrum disorder

LI Dexin¹, CHEN Qiang¹, ZHUANG Zhicheng¹, CHEN Hong¹, YANG Bingjiao¹, JIANG Shunxiang², ZHOU Xiang¹

1. Department of Children Psychological Health, Zhuhai Maternal and Child Health Care Hospital, Zhuhai, Guangdong 519001, China;
2. BGI Precision Nutrition (Shenzhen) Technology Co.Ltd,

Received:2022-10-13 Revised:2023-02-07 Online:2023-05-10 Published:2023-05-06
Contact: ZHOU Xiang, E-mail: zhouxiangp@163.com

摘要/Abstract

摘要： 目的分析孤独症谱系障碍(ASD)儿童肠道菌群结构特点及与症状严重程度的相关性,以期为ASD发病机理及靶向治疗提供新思路。方法选取2021年3月—2021年9月在珠海市妇幼保健院儿童心理卫生科就诊的107名ASD儿童作为ASD组,纳入幼儿园正常儿童30名作为对照组。采用儿童孤独症评定量表评估ASD症状严重程度。收集两组儿童新鲜粪便并提取样本DNA,对细菌16S rDNA V4区进行扩增并进行高通量测序。结果 ASD组和对照组儿童的肠道菌群α多样性指数和β多样性差异无统计学意义(P＞0.05);在门水平,ASD组儿童变形菌门相对丰度较对照组儿童低,且差异具有统计学意义(t=-3.103,P＜0.05);在科水平,ASD组儿童的优势菌群为坦纳氏菌科、爱格士氏菌科、脱硫弧菌科、莫拉氏菌科、月形单胞菌科、双歧杆菌科;正常儿童的优势菌群为恩斯氏菌科、链球菌科、巴斯德氏菌科;在属水平,不同症状严重程度儿童丁酸弧菌属、柯林斯氏菌属、布劳特氏菌属、巨单胞菌属相对丰度差异均有统计学意义(P＜0.05);Spearman相关性分析显示,ASD儿童布劳特氏菌属和巨单胞菌属与CARS评分呈正相关(r=0.213、0.288,P＜0.05)。结论 ASD儿童存在肠道菌群紊乱,且肠道菌群结构与行为症状的严重程度密切相关。

关键词: 孤独症谱系障碍, 肠道菌群, 高通量测序

Abstract: Objective To analyze structural characteristics of gut microbiota and its correlation with symptom severity in children with autism spectrum disorder (ASD), in order to provide new ideas for exploring pathogeny and targeted therapy. Methods A total of 107 ASD children in the Child Mental Health Department of Zhuhai Maternal and Child Health Hospital from March 2021 to September 2021 were selected as the ASD group, and 30 normal preschool children in a kindergarten were included as the control group.The severity of ASD symptoms was assessed using the Childhood Autism Rating Scale (CARS).Sample DNA was extracted after collecting fresh stools from both groups of children, and then the 16S rDNA V4 region of the bacteria was amplified and sequenced in high throughput. Results There was no significant difference between the alpha diversity index and beta diversity of the gut microbiota between children in ASD group and control group (P>0.05).At the phylum level, the relative abundance of the Proteobacteria in the ASD group was significantly lower than that in the control group (t=-3.103, P<0.05).At the family level, the dominant gut microbiota of children in ASD group included Tannerellaceae, Eggerthellaceae, Desulfovibrionales, Selenomonadaceae, Moraxellaceae, Bifidobacterium, while the dominant gut microbiota in normal group included Barnesiellaceae, Streptococcus, Barnesiella.At the genus level, the differences in the relative abundance of Anaerostipes, Collinsella, Blautia, and Megamonas in children with different severity of symptoms were statistically significant (P<0.05).Spearman correlation analysis showed a positive correlation of Blautia and Megamonas with CARS score in ASD children (r=0.213, 0.288, P<0.05). Conclusion Gut microbiota of ASD children is disturbed and its structure is strongly related to the severity of behavioral symptoms.

Key words: autism spectrum disorder, gut microbiota, high-throughput sequencing

中图分类号:

R179

李德欣, 陈强, 庄志成, 陈红, 杨秉娇, 姜顺香, 周翔. 孤独症谱系障碍儿童肠道菌群与症状严重程度相关性研究[J]. 中国儿童保健杂志, 2023, 31(5): 497-501.

LI Dexin>, CHEN Qiang>, ZHUANG Zhicheng>, CHEN Hong>, YANG Bingjiao>, JIANG Shunxiang>, ZHOU Xiang>. Correlation between gutmicrobiota and symptom severity in children with autism spectrum disorder[J]. Chinese Journal of Child Health Care, 2023, 31(5): 497-501.

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孤独症谱系障碍儿童肠道菌群与症状严重程度相关性研究

Correlation between gutmicrobiota and symptom severity in children with autism spectrum disorder

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