单纯性肥胖儿童载脂蛋白E基因多态性的频率分布及其对血脂的影响研究

中国儿童保健杂志 ›› 2011, Vol. 19 ›› Issue (6): 524-527.

单纯性肥胖儿童载脂蛋白E基因多态性的频率分布及其对血脂的影响研究

王四美,龚群,胡立群,王瑜,徐淼

上海市儿童医院,上海交通大学附属儿童医院,儿童保健所,上海 200040

收稿日期:2011-03-13 发布日期:2011-06-06 出版日期:2011-06-06
通讯作者: 龚群,E-mail:gongqun684@163.com
作者简介:王四美(1982-),女,河南人,在读硕士,主要从事儿童肥胖的基础与临床研究
基金资助:
上海市卫生局课题资助项目(2006058)

Study on the frequency distribution of apolipoproteinE gene polymorphism of obesity children and its effection on lipid levels in plasma

WANG Si-mei , GONG Qun, HU Li-qun, WANG Yu, XU Miao

Shanghai Child Health Care Institute, Shanghai Children's Hosptial, Shanghai Jiaotong University, Shanghai 200040, China

Received:2011-03-13 Online:2011-06-06 Published:2011-06-06

摘要/Abstract

摘要： 【目的】探讨单纯性肥胖儿童载脂蛋白E(apolipoprotein E, ApoE)基因多态性的频率分布及对其血脂谱水平的影响。【方法】采用基因测序方法对100例单纯性肥胖儿童及100例正常对照组儿童进行ApoE基因多态性测定,另外抽取空腹静脉血作甘油三酯(triglyceride, TG)、胆固醇(total cholesterol, TC)、高密度脂蛋白(high-density lipoprotein cholesterol, HDL)、低密度脂蛋白(low-density lipoprotein cholesterol, LDL)、载脂蛋白A(apolipoprotein A, ApoA)、载脂蛋白B(apolipoprotein B, ApoB)检测,观察不同基因型和等位基因的分布情况,并与血脂的相关性进行分析。【结果】基因型E3/3频率分布最高,在70%之上,而含E3的杂合子(E2/3和E3/4)居中,二者之和超过20%,E2/2和E4/4表型的频率最低;与对照组比较,实验组肥胖儿童E2型等位基因有较高水平的TG(P＜0.05),E3型有较高的TG、TC、LDL水平和较低HDL、ApoA水平(P＜0.01),E4型有较高的TG、AopB水平和较低的HDL、ApoA水平(P＜0.05);肥胖组儿童中,与E3相比较,E4具有较高的TG、ApoB值和较低的HDL值,差异有统计学意义(P＜0.05),而E2具有较低的TC、LDL和ApoB水平,但是差异无统计学意义;携带不同等位基因的肥胖儿童的血脂异常率为E4＞E3＞E2(P＜0.01)。【结论】 ApoE3/3是分布频率最高的基因型,ApoE4等位基因是血脂异常的风险基因之一,与肥胖儿童的血脂异常存在显著相关性。

关键词: 载脂蛋白E, 肥胖儿童, 基因多态性, 血脂

Abstract: 【Objective】 To explore the frequency distribution of apolipoproteinE(ApoE) gene polymorphism of obesity children and its effection on lipid levels in plasma. 【Methods】 Using DNA sequencing method to detect the apolipoproteinE gene polymorphism of 100 obesity children and 100 normal children of control group. Moreover, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), apolipoprotein A(apoA) and apolipoprotein B(apoB) were detected with the 12 h fasting blood. Observing the distribution of different genotypes and allele, and analysis of its correlation with lipid. 【Results】 E3/3 genotype frequence(＞70%) of ApoE was quite higher than E3 heterozygous (E2/3 and E3/4＞20%) and E2/2and E4/4; compared to the control group, E2 allele of the experimental group had higher level of TG(P＜0.05), E3 allele had higher level of TG, TC, LDL and lower level of HDL, ApoA(P＜0.01), while E4 allele had higher level of TG, AopB and lower level of HDL, ApoA(P＜0.05), the differences had statistical significance; in experimental group, compared to E3 allele, the child who carry E2 allele had lower level of TC, LDL and ApoB(P＞0.05), E4 allele has higher level of TG, ApoB and lower HDL(P＜0.05); the obesity children who carry different allele had different dyslipidemia rate, it was E4＞E3＞E2(P＜0.01). 【Conclusion】 The apoE3/3 is the most common genotype, and ApoE4 are correlated with dyslipidemia of obesity children and is one of the risk genes of dyslipidemia.

Key words: apolipoproteinE, obesity children, polymorphism, lipid

中图分类号:

R179

王四美,龚群,胡立群,王瑜,徐淼. 单纯性肥胖儿童载脂蛋白E基因多态性的频率分布及其对血脂的影响研究[J]. 中国儿童保健杂志, 2011, 19(6): 524-527.

WANG Si-mei , GONG Qun, HU Li-qun, WANG Yu, XU Miao. Study on the frequency distribution of apolipoproteinE gene polymorphism of obesity children and its effection on lipid levels in plasma[J]. journal1, 2011, 19(6): 524-527.

参考文献

[1] Csahi G, Torok K, Jeges S, et al. Presence of metabolic cardiovaseular syndrome in obese children[J]. Eur J Pediatr,2000,159(1-2):91-94.
[2] Lin SK, Kao JT, Tsai SM, et al. Association of apolipoproteinE genotypes with serum lipid profiles in a healthy population of Taiwan[J]. Ann Clina Lab Sci,2004,34(4):443-448.
[3] Wu K, Bowman R, Welch AA, et al. Apolipoprotein E polymorphisms, dietary fat and fibre, and serum lipids. The EPIC Norfolk study[J]. Eur Heart J,2007,28(23):2930-2935.
[4] Bazzaz JT, Nazari M, Nazem H, et al. Apolipoprotein E gene polymorphism and total serum cholesterol level in Iranian population[J]. J Postgrad Med,2010,56(3):173-177.
[5] Fernandez-Mestre MT, Yehirobi C, Montagnani S, et al. Genetic variability of apolipoprotein E in different populations fromVenezuela[J]. Dis Markers,2005,21(1):15-23.
[6] Al-Khedhairy AA. Apolipoprotein E polymorphism in Saudis[J]. Mol Biol Rep,2004,31(4):257-260.
[7] Yang SL, He BX, Liu HL, et al. ApolipoproteinE gene polymorphisms and risk for coronary artery disease in Chinese Xinjiang Uygur and Han population[J]. Chin Med Sci J,2004,19(2):150-154.
[8] Tang H, Yan X, Hua Y, et al. Distribution of apoE polymorphism in Chinese Yunnan Dehong Dai ethnic group[J]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi,2005,22(2):224-229.
[9] 王四美,胡立群,龚群,等.环境与家系因素对单纯性肥胖儿童血脂谱水平的影响研究[J].中国儿童保健杂志,2010,18(12):934-937.
[10] Allemand D, Wiegand S, Reinehr T, et al. Cardiovascular risk in 26,008 European overweight children as established by a multicenter database[J]. Obesity,2008,16(7):1672-1680.
[11] 鲁然.载脂蛋白E的多态性与基因检测[J].淮海医药,2006,24(6):538-539.
[12] Iordanes K, Rami A, Aikatemini T, et al. Apolipoprotein E predisposes to obesity and related metabolic dysfunctions in mice[J]. FEBS Journal,2008,275(19):4796-4809.
[13] 王莲,刘长云,赵园园,等.单纯性肥胖儿童脂代谢与LEPR及ApoE基因多态性的相关性研究[J].中华妇幼临床医学杂志,2007,3(4):189-193.
[14] Trsen U, Eskandari G, Kaya TI, et al. Apolipoprotein E polymorphism and lipoprotein compositions in normolipidaemic xanthelasma patients[J]. J Eur Acad Dermatol Nereol,2006,20(3):260-263.
[15] Rafael OA, Silvia RSF, Noely EF, et al. APOE polymorphism is associated with lipid profile, but not with arterial stiffness in the general population[J]. Lipids Health Dis,2010,9:128-132.
[16] Sima A, Lordan A, Stancu C. Apolipoprotein E polymorphism-a risk factor for metabolic syndrome 1[J]. Clinical Chemistry & Laboratory Medicine,2007,45(9):1149-1153.
[17] National Cholesterol Education Problem(NECP). Highlights of the report of the expert panel on blood cholesterol levels in children and adolescents[J]. Pedistrics,1992,89(3):495-501.