中国儿童保健杂志 ›› 2012, Vol. 20 ›› Issue (9): 800-802.

• 科研论著 • 上一篇    下一篇

儿童血清中性粒细胞明胶酶相关脂质运载蛋白与维生素A代谢指标相关性研究

陈科1,章岚1,程昕然1,罗红裔1,高宁1,王劲1,傅桂英1,毛萌2   

  1. 1 成都市妇女儿童中心医院儿童保健并儿童与青少年生长发育诊治中心,四川 成都 610031;
    2 四川大学华西第二医院教育部妇儿疾病与出生缺陷重点实验室,四川 成都 610081
  • 收稿日期:2011-12-27 发布日期:2012-09-06 出版日期:2012-09-06
  • 通讯作者: 毛萌,E-mail:dffmmao@126.com
  • 作者简介:陈科(1976-),男,四川人,主治医师,博士学位,研究方向为儿童营养及生长发育
  • 基金资助:
    瑞士SIGHT AND LIFE国际维生素A研究基金(20090221)

Relationship between serum neutrophil gelatinase-associated lipocalin and vitamin A metabolic index in preschool children

CHEN Ke1,ZHANG Lan1,CHENG Xin-ran1,LUO Hong-yi1,GAO Ning1,WANG Jin1,FU Gui-ying1,MAO Meng2   

  1. 1 Department of Child Health Care and Child and Youth Growth and Development Treatment Center,Chengdu,Sichuan 610031,China;
    2 Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education,West China Second Hospital,Sichuan University,Chengdu,Sichuan 610081,China
  • Received:2011-12-27 Online:2012-09-06 Published:2012-09-06

摘要: 【目的】 初步分析中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin,NGAL)水平与维生素A代谢指标血清视黄醇、视黄醇结合蛋白 (retinol-binding protein,RBP)、甲状腺素运载蛋白(transthyretin,TTR)的关系。 【方法】 随机抽取研究现场8所幼儿园473名2~7岁(不足7岁)学龄前儿童纳入。分别利用HPLC法测血清视黄醇;采用ELISA法测NGAL与 RBP,采用免疫比浊法测TTR,并计算R-T指数[RBP(mol/L)/TTR(mol/L)]。 【结果】 血清NGAL与血清视黄醇和R-T指数存在明显负相关关系(偏相关系数r分别为0.21和0.25;P均<0.000 1)。维生素A正常组儿童NGAL水平显著低于维生素A不正常组儿童(P<0.05)。以指标中位数为界,低R-T指数组儿童血清NGAL水平明显高于高R-T组儿童(P<0.05),但是高与低RBP组儿童之间以及高与低TTR组儿童之间血清NGAL水平差异均无统计学意义(P>0.05)。分别以血清NGAL水平的P25,P50以及P75浓度为界,将研究对象分为Q1,Q2,Q3和Q4组,Q1组视黄醇水平和R-T指数均显著高于Q4组(P<0.05),血清RBP和TTR水平则无明显组间差异(P>0.05)。 【结论】 学龄前儿童血清NGAL水平与机体维生素A存在密切相关,维生素A可能可以通过抑制NGAL的表达而对抗NGAL对造血的抑制。

关键词: 中性粒细胞明胶酶相关脂质运载蛋白, 维生素A, 学龄前儿童, 贫血

Abstract: 【Objective】 To explore the correlation of neutrophil gelatinase-associated lipocalin (NGAL) and vitamin A metabolic index,including serum retinol,serum retinol binding protein (RBP),serum transthyretin (TTR) and the molar ratio of RBP to TTR[R-T index,RBP(mol/L)/TTR(mol/L)]. 【Methods】 About 473 preschool children with 2~7 years old were randomly selected from eight kindergartens,including 223 boys and 250 girls with (47.8±14.5) months.Serum retinol was measured by HPLC method,NGAL and RBP by ELISA and TTR by immunoturbidimetric method. 【Results】 There were significant negative correlations between NGAL and retinol and R-T index(The partial correlation coefficients were 0.21 and 0.25;P all<0.000 1).The value of serum NGAL in children with normal vitamin A was markedly lower than that of children with unnormal vitamin A (P<0.05).Children with low R-T index level (cut by R-T index media value) had higher serum NGAL level than those with high R-T index level (P<0.05);However,no significant difference was observed between children with high and low RBP/TTR levels (P>0.05).The serum retinol and R-T index levels in children with Q1 RBP value group (cut by RBP 4 quartile level) was significantly higher than those of children with Q4 RBP value group (P<0.05),but not for serum RBP and TTR values (P>0.05). 【Conclusion】 There is close relation between serum NGAL and vitamin A status in preschool children and vitamin A may be able to inhibit the expression of NGAL which against the inhibition of NGAL on hematopoietic system.

Key words: neutrophil gelatinase-associated lipocalin, vitamin A, preschool children, anemia

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