关键词: 支气管哮喘, VDR基因, 单核昔酸多态, 儿童

Abstract: Objective To discuss the differences between Ningxia Hui and Han asthmatic children about the single nucleotide polymorphisms of locus of BsmI,ApaI,TaqI and FokI on the VDR gene. Methods The peripheral blood DNA from Hui and Han asthmatic children was extracted to analyze the genotype and gene frequency of dynamic locus of VDR gene using the methods of PCR and PCR-RFLP,Chi square test was used to analyse data. Results FokI was a locus where the difference of asthma existed between Ningxia Hui and Han asthmatic children(P<0.05).There was no distinguished difference about the distribution of gene frequency and genotype frequency of the locus of BsmI,ApaI and TaqI existed between the Hui asthma group and Han asthma group(P>0.05). Conclusions Among the four polymorphic sites being studied,the following results have been achieved:FokI is a locus where the differences exist between Ningxia Hui children's asthma and Ningxia Han children's asthma.There is no difference between Ningxia Hui and Han children's asthma about the locus of BsmI,ApaI and TaqI.

Key words: bronchial asthma, VDR gene, single nucleotide polymorphism, children