关键词: 支气管哮喘, T细胞亚群, 儿童

Abstract: Objective To explore the expression of T-lymphocyte subsets in peripheral blood of children with asthmatic disease. Method Flow cytometry (FCM) was applied to measure the expression of T-lymphocytes in children within acute asthma group,asthmatic pneumonia group and healthy control group. Results Percentages of CD3⁺ lymphocytes of acute asthma group and asthmatic pneumonia group were significantly lower than that of healthy control group (P<0.01),with no statistical variance found between acute asthma group and asthmatic pneumonia group (P>0.05).Compared to healthy control group,percentage of CD4⁺ lymphocytes in acute asthma group and asthmatic pneumonia group were significantly higher (P<0.05),with significant differences between asthmatic pneumonia group and healthy control group.Compared with healthy control group,percentages of CD8⁺ lymphocytes was significantly lower than that in acute asthma group and asthmatic pneumonia group (P<0.05),with no significant difference between acute asthma group and asthmatic pneumonia group (P>0.05).CD3^-CD19⁺and CD19⁺CD23⁺ lymphocytes of acute asthma group and asthmatic pneumonia group were markedly increased than those of healthy control group (P<0.05),but variances of these two subsets in exacerbation asthma group and asthmatic pneumonia group were not statistically significance (P>0.05).Conclusion Cellular immunity disorder was involved in the pathogenesis in children with asthma and asthmatic pneumonia.

Key words: bronchial asthma, T-lymphocyte subsets, children