胸腺和活化调节趋化因子在哮喘大鼠中的作用及布地奈德干预的影响

中国儿童保健杂志 ›› 2014, Vol. 22 ›› Issue (3): 268-270.

胸腺和活化调节趋化因子在哮喘大鼠中的作用及布地奈德干预的影响

王优¹, 黄秀兰¹, 苏赞彩¹, 王苑², 谢朝阳³, 谭建新¹

广东医学院附属医院1 儿科, 2 药剂科, 广东湛江 524001; 3 广东医学院检验学院, 广东湛江 524001

收稿日期:2013-05-24 发布日期:2014-03-05 出版日期:2014-03-05
作者简介:王优(1968-), 女, 主任医师, 硕士研究生, 主要研究方向为呼吸系统疾病。
基金资助:
湛江市科技攻关立项课题(2007C05001)

Roles of thymus and activation regulated chemokine in asthmatic rats and interference of budesonide.

WANG You¹, HUANG Xiu-lan¹, SU Zan-cai¹, WANG Yuan², XIE Chao-yang³, TAN Jian-xin¹.

1 Department of Pediatrics;2 Department of Pharmacy, the Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, China;3 Laboratory of Guangdong Medical College, Zhanjiang, Guangdong 524001, China

Received:2013-05-24 Online:2014-03-05 Published:2014-03-05

摘要/Abstract

摘要： 目的探讨胸腺和活化调节趋化因子(thymus and activation regulated chemokine, TARC)在哮喘大鼠中的作用及吸入布地奈德干预对TARC的影响。方法 30只SD大鼠采用随机区组设计分为正常对照组、哮喘组及布地奈德干预组。以卵清白蛋白(ovalbumin, OVA)致敏激发法制备大鼠哮喘急性模型, 干预组于14 d雾化吸入布地奈德进行干预。于最后1次雾化后24 h, 尾静脉取血, 支气管肺泡灌洗留取灌洗液(bronchoalveolar lavage fluid, BALF), 留取右肺组织标本。行血清和BALF中细胞计数, 应用酶联免疫吸附(ELISA)法测定血清和BALF中上清液中嗜酸粒细胞(Eos)、IL-4及TARC的水平。采用免疫组化法测定肺组织中TARC及IL-4蛋白的表达。结果 1) 哮喘组大鼠血清及BALF中的Eos、IL-4和TARC水平均高于对照组(P<0.05);肺组织中IL-4及TARC的蛋白表达也高于对照组(P<0.05);布地奈德干预后上述各项指标值均低于哮喘组(P<0.05), 布地奈德干预组的各项指标与对照组相比差异无统计学意义(P>0.05)。2)哮喘组大鼠肺组织中TARC的蛋白表达与IL-4呈正相关(r=0.50, P<0.05)。结论哮喘大鼠中TARC含量明显增高, 吸入布地奈德可降低TARC的水平。提示TARC在哮喘的发病中起重要的作用, 下调TARC可能是激素治疗哮喘的一个重要机制。

关键词: 胸腺和活化调节趋化因子, 哮喘, 布地奈德, 大鼠

Abstract: Objective To explore the role of thymus and activation regulated chemokine(TARC) in asthmatic rats and therapeutic effects of budesonide. Methods A total of 30 SD rats were randomly divided into normal group, asthmatic group, budesonide treated group, the rat model of asthma was established by the ovalbumin (OVA) provocation methods.The blood and bronchoalveolar lavage fluid (BALF) and lung tissue were collected 24 h after the last inhaling budesonide.The cells were counted, the concentrations of IL-4 and TARC in serum and BALF were measured by enzyme-linked immunosorbent assay (ELISA).The IL-4 and TARC protein in lung tissue were detected by immnohistochemical method. Results 1)The counts of eosinophiles, the IL-4 and TARC levels in serum and BALF of asthmatic groups were significantly higher than those in normal group(P<0.05), and the expressions of IL-4 and TARC protein in lung tissue of asthmatic groups were obviously higher than those in normal groups (P<0.05);They were significantly increased when inhaling budesonide (P<0.05).There were no significances between the control groups and the budesonide treated groups (P>0.05).2)Significant association was found between expression of TARC and IL-4 in the lung tissue of asthma groups and they presented for positive correlations (r=0.50, P<0.05). Conclusions TARC levels were increased in asthmatic rats.Inhaling budesonide can decrease the levels of TARC, TARC plays an important role in the pathogenesis of asthma.Downregulation of TARC expression may be the important mechanism of budesonide to treat asthma.

Key words: thymus and activation regulated chemokine, asthma, budesonide, rats

中图分类号:

Q95-33

王优, 黄秀兰, 苏赞彩, 王苑, 谢朝阳, 谭建新. 胸腺和活化调节趋化因子在哮喘大鼠中的作用及布地奈德干预的影响[J]. 中国儿童保健杂志, 2014, 22(3): 268-270.

WANG You, HUANG Xiu-lan, SU Zan-cai, WANG Yuan, XIE Chao-yang, TAN Jian-xin.. Roles of thymus and activation regulated chemokine in asthmatic rats and interference of budesonide.[J]. journal1, 2014, 22(3): 268-270.

参考文献

[1] Han SX, Han XM, Wu XM, et al.Investigation of eosinophil apoptosis in asthma patients[J].China Journal of Modem Medicine, 2004, 14(7):15-22.
[2] Imai T, Yoshida T, Baba M, et al.Molecular cloning of a novel Tcell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector[J].J Biol Chem, 1996, 271(35):21514-21521.
[3] Asojo OA, Boulegue C, Hoover DM, et al.Structures of thymus and activation-regulated chemokine(TARC)[J].Acta Crystallogr D Biol Crystallogr, 2003, 59(Pt7):1165-1173.
[4] Sekiya T, Yamada H, Yamaguchi M, et al.Increased levels of a TH2-type CC chemokine thymus and activation -regulated chemokine(TARC) in serum and induced sputum of asthmatics[J].Allergy, 2002, 57(2):173-177.
[5] Leung TF, Wong CK, Chan IH, et al.Plasma concentration of thymus and activation-regulated chemokine is elevated in childhood asthma[J].J Allergy Clin Immunol, 2002, 110(3):404-409.
[6] Sekiya T, Miyamasu M, Imanishi M, et al.Inducible expression of a Th2-type CC chemokine thymus-and activation-regulated chemokine by human bronchial epithelial cells[J].J Immunol, 2000, 165(4):2205-2213.
[7] Panina-Bordignon P, Papi A, Mariani M, et al.The C-C chemokine receptors CCR4 and CCR8 identify airway T cells of allergen-challenged atopic asthmatics[J].J Clin Invest, 2001, 107(11):1357-1364.
[8] Miyazaki E, Nureki S, Fukami T, et al.Elevated levels of thymus-and activation-regulated chemokine in bronchoalveolar lavage fluid from patients with eosinophilic pneumonia[J].Am J Respir Crit Care Med, 2002, 165(8):1125-1131.
[9] Zee RY, Fernandez, Ortiz A.ACE D/I polymorphism andincidence of post-PTCA restenosis:a prospective angiography-based evaluation[J].Hypertension, 2001, 37(3):851-855.
[10] Kurokawa M, Kokubu F, Matsukurn S, et al.Effects of corticosteroid on the expression of thymus and activation-regulateel chemokine in a murine model of allergic asthma[J].Int Arch Allergy lmmunol, 2005, 137 (S):60-68.
[11] Hoshino M, Nakagawa T, Sano Y, et al.Effect of inbaled cortieosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics[J].A1lergy, 2005, 60(3):317-322.

[1]	买国华. 学龄期支气管哮喘患儿自我管理行为调查及影响因素[J]. 中国儿童保健杂志, 2023, 31(4): 446-450.
[2]	李玉, 赵嘉虹, 阚璇. 中国儿童哮喘行动计划的临床应用研究[J]. 中国儿童保健杂志, 2023, 31(2): 185-189.
[3]	蔡冬, 周丽霞, 许花芬. MiR-223-3p通过靶向NLRP3对坏死性小肠结肠炎新生大鼠肠组织的炎症反应和细胞损伤的影响[J]. 中国儿童保健杂志, 2023, 31(1): 46-51.
[4]	徐燕, 刘东海, 梁譞, 郭丽, 戴永利, 张蓉芳. 高原地区儿童哮喘影响因素分析[J]. 中国儿童保健杂志, 2022, 30(7): 796-800.
[5]	张瑜, 谢小敏, 马田, 仝海霞, 王蕾, 李鸽, 于夏, 尚磊, 江逊. 青春期哮喘患儿疾病控制与家庭功能、自我管理、知觉压力的相关性研究[J]. 中国儿童保健杂志, 2022, 30(3): 314-318.
[6]	李玉, 阚璇. 细菌溶解产物在儿童呼吸道过敏性疾病应用的研究进展[J]. 中国儿童保健杂志, 2022, 30(12): 1336-1340.
[7]	张洋, 王慧渊, 耿妍, 金巧艳, 郝娟娟, 侯伟. 支气管哮喘儿童情绪问题现状及相关因素分析[J]. 中国儿童保健杂志, 2022, 30(12): 1400-1403.
[8]	杨丽娟, 邓高, 丁波, 杨丽敏, 李东明, 黄永方, 林娜. ADAM33基因及其血清水平与广西壮族儿童哮喘发病风险分析[J]. 中国儿童保健杂志, 2022, 30(11): 1185-1190.
[9]	李婉莹, 宋春兰, 单照茜, 熊蕾蕾, 张志英. 不同控制水平哮喘患儿心血管代谢指标的变化及意义[J]. 中国儿童保健杂志, 2022, 30(11): 1246-1249.
[10]	富妍妍, 刘伟, 徐丽媛. 郊区和城区空气污染物浓度差异及与儿童哮喘患病情况的关系[J]. 中国儿童保健杂志, 2022, 30(11): 1262-1266.
[11]	沈仁, 杨善浦, 张林桃, 吴月超. 儿童反复喘息与4位点哮喘基因预测模型的关系及危险因素分析[J]. 中国儿童保健杂志, 2022, 30(10): 1132-1135.
[12]	李昱剑, 王杨, 阚璇. 中国儿童哮喘急性发作危险因素的Meta分析[J]. 中国儿童保健杂志, 2021, 29(9): 992-995.
[13]	刘阳, 高伟霞, 宋哲, 朱永杰. 呼出气一氧化氮联合白介素-17、白介素-27检测对儿童支气管哮喘的临床诊断价值[J]. 中国儿童保健杂志, 2021, 29(9): 1008-1011.
[14]	赵铭, 秦苗, 孙梦雅, 姜红, 李向红, 姜健, 刘燕. NLRP3及相关炎性因子在新生大鼠坏死性小肠结肠炎中的表达研究[J]. 中国儿童保健杂志, 2021, 29(8): 852-856.
[15]	张耀云, 刘菊芬. 围孕期增补叶酸对子代哮喘及过敏性疾病的影响[J]. 中国儿童保健杂志, 2021, 29(8): 861-864.