ω-3PUFAs、ω-6PUFAs和ω-9MUFAs对LPS诱导急性肺损伤大鼠血清IL-6及细胞凋亡的影响

中国儿童保健杂志 ›› 2014, Vol. 22 ›› Issue (3): 271-274.

ω-3PUFAs、ω-6PUFAs和ω-9MUFAs对LPS诱导急性肺损伤大鼠血清IL-6及细胞凋亡的影响

石计朋¹, 闫建国², 韩金芬³, 唐成和¹, 余震⁴

1 新乡医学院第一附属医院新生儿科, 河南新乡 453003;
2 新乡医学院人体解剖教研室, 河南新乡 453003;
3 新乡医学院第三附属医院新生儿科, 河南新乡 453003;
4 温州医科大学附属第一医院胃肠外科, 浙江温州 325000

收稿日期:2013-07-19 发布日期:2014-03-05 出版日期:2014-03-05
通讯作者: 余震, E-mail:yuzhen0577@163.com
作者简介:石计朋(1981-), 男, 河南人, 硕士学位, 研究方向为新生儿疾病。
基金资助:
浙江省医学重点扶植学科“临床营养学(2007-F03)”

Effect of three kinds of lipid emulsions on the IL-6 expression on acute lung injury in young rats induced by endotoxin.

SHI Ji-peng¹, YAN Jian-guo², HAN Jin-fen³, TANG Cheng-he¹, YU Zhen⁴.

1 Department of Neonatology, the First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, China; 2 Department of Anatomy, Xinxiang Medical University, Xinxiang, Henan 453003, China; 3 Department of Paediatrics, the Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453003, China; 4 Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China

Received:2013-07-19 Online:2014-03-05 Published:2014-03-05
Contact: YU Zhen, E-mail:yuzhen0577@163.com

摘要/Abstract

摘要： 目的比较ω-3PUFAs、ω-6PUFAs和ω-9MUFAs三种脂肪乳剂对脂多糖(lipid emulsions, LPS)诱导的急性肺损伤大鼠IL-6分泌的影响。方法 100只大鼠按随机数字分为Control组、LPS组、Intra组、Clino组和Omega组。分别采用生理盐水或不同种类脂肪乳剂处理7 d;并均在取标本前8 h时于气管内滴入生理盐水或LPS, 建立ALI大鼠模型;观察各组大鼠肺组织的病理改变、凋亡指数, 测定IL-6mRNA变化以及IL-6蛋白浓度。结果 1)各组大鼠肺组织病理切片均可见明显炎症细胞浸润和出血;2)Clino组和Omega组血清中IL-6mRNA表达较Intra组、LPS组均明显降低;BALF中Clino组和Omega组IL-6蛋白水平明显低于Intra组、LPS组, 差异具有统计学意义(P均＜0.005)。而Clino组和Omega组两组IL-6mRNA表达及其蛋白水平差异均无统计学意义(P均＞0.005)。3)Clino组和Omega组凋亡系数均低于LPS组和Intra组(P均<0.005);Clino组和Omega组AI值差异无统计学意义(P＞0.005)。结论 Clinoleic和Omegaven可通过降低IL-6而减轻ALI大鼠炎症反应, 并能减轻肺泡上皮细胞凋亡。

关键词: 脂多糖, 急性肺损伤, 白介素6

Abstract: Objective To compare the effects of three kinds of lipid emulsions to IL-6, and alveolar epithelial cell apoptosis of lipid emulsions (LPS)-induced rat lung of inflammatory. Methods One hundered young Sprague-Dawley rats were divided into control group, LPS group, intra group, Clino group and Omega group and then used normal saline or different fat emulsions for seven days.LPS was installed in order to establish the rat model of acute lung injury (ALI).Those rats were intratracheally instilled with normal saline or LPS 8 hours before sacrifice.Each lung tissue section's pathologic changes was observed, and apoptosis index(AI) was detected.Expression level of IL-6 mRNA was examined by RT-PCR, meanwhile the concentrations of IL-6 in BALF were measured by enzyme-linked immunospecific assay (ELISA). Results 1)The expressions of IL-6 mRNA in serum and the level of IL-6 in BALF of LPS groups and intra group were significantaly elevated than clino group and omega group (both P<0.005), but it was no significant difference(P＞0.005)when Clino group compared with Omega group.2)pulmonary histopathology:there were demonstrated significant inflammatory in light microscopy of HE-stained lungs tissues of rat, and they were showing inflammatory cells infiltrating around the bronchus and the vascular, even bleeding.3)apoptosis results:compared with LPS group and intra group, the AI of Clino group and Omega group significantly reduced(both P<0.005), which was no significant difference between each other(P＞0.005). Conclusion 20% clinoleic and 10% omegaven can relieve inflammation by downregulating the concentrations of IL-6, and reduce the alveolar epithelial cell apoptosis in rats with acute lung injury.

Key words: lipid emulsions, acute lung injury, IL-6

中图分类号:

Q954.5

石计朋, 闫建国, 韩金芬, 唐成和, 余震. ω-3PUFAs、ω-6PUFAs和ω-9MUFAs对LPS诱导急性肺损伤大鼠血清IL-6及细胞凋亡的影响[J]. 中国儿童保健杂志, 2014, 22(3): 271-274.

SHI Ji-peng, YAN Jian-guo, HAN Jin-fen, TANG Cheng-he, YU Zhen.. Effect of three kinds of lipid emulsions on the IL-6 expression on acute lung injury in young rats induced by endotoxin.[J]. journal1, 2014, 22(3): 271-274.

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