关键词: 茶多酚, 人支气管上皮细胞, 脂多糖, 氧化应激

Abstract: Objective To investigate the effect of Tea Polyphenols(TP) on lipopolysaccharide (LPS)-induced human bronchial epithelial cell injury and underlying mechanism. Methods Cultured human bronchial epithelial cells (16HBE) were treated with LPS (1,25,50,100 μg/ml) and TP (100,200,400,800 g/ml),respectively.Cell proliferation was measured by MTT method.Cell apoptosis was detected by flow cytometry assay.The expression levels of nuclear NFκB and NFκB were measured by western blot.The content of IL-6,IL-13 and TNF-α were detected by Elisa method.The MDA content,LDH content,SOD activity were measured by corresponding kit. Results Compared with the control group,the cell proliferation was significantly decreased; cell apoptosis was increased;the content of IL-6,IL-13,TNF-α were increased;protein expression of nuclear NFκB,NFκB were increased; the content of MDA,LDH were increased,activity of SOD was decreased.LPS induced 16HBE injury was improved by TP treatment. Conclusion tea polyphenols attenuates lipopolysaccharide-induced human bronchial epithelial cell injury through inhibiting oxidative stress.

Key words: tea polyphenols, human bronchial epithelial cell, lipopolysaccharide, oxidative stress