孕期鼠补充维生素D对子鼠胸腺维生素D受体表达

doi:10.11852/zgetbjzz2016-24-07-11

中国儿童保健杂志 ›› 2016, Vol. 24 ›› Issue (7): 712-714.DOI: 10.11852/zgetbjzz2016-24-07-11

孕期鼠补充维生素D对子鼠胸腺维生素D受体表达

王志会,赵霞霞,任立红

哈尔滨医科大学附属第二医院儿内科,黑龙江哈尔滨 158086

收稿日期:2016-03-01 发布日期:2016-07-10 出版日期:2016-07-10
通讯作者: 任立红,E-mailrenlihong1@126.com
作者简介:王志会(1986-),男,在读硕士,研究方向为维生素D与免疫性疾病。
基金资助:
黑龙江省自然科学基金项目 (H201397)

Effect of vitamin D receptor expression in the thymus of filial generation mice after giving pregnant vitamin D.

WANG Zhi-hui,ZHAO Xia-xia,REN LI-hong.

Department of Pediatric,the Second Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang 158086,China

Received:2016-03-01 Online:2016-07-10 Published:2016-07-10
Contact: REN Li-hong,E-mail:renlihong1@126.com

摘要/Abstract

摘要： 目的研究给予母孕期大鼠补充不同剂量维生素D,检测子代鼠胸腺中VDR表达变化。方法健康雌性Wistar大鼠18只,雄性Wistar大鼠9只,以每日晚间17时将雌鼠、雄鼠按2∶1合笼,次日8时对雌鼠阴道分泌物涂片,在40倍光学显微镜检查到精子表示受孕,记为妊娠0 d( gestational day 0,GD0) 。将孕鼠随机入对照组、维生素D低剂量、维生素D高剂量组。于GD 15 d起对照组每日给予肌肉注射生理盐水0.25 ml/(kg·次),低剂量组给予肌肉注射维生素D 20 μg/kg,高剂量组给予肌肉注射维生素D 100 μg/kg,直至子鼠娩出。子鼠哺乳21 d后分离子鼠胸腺,Real-time PCR检测VDRmRNA表达水平,Western blot检测VDR蛋白表达水平。结果通过Real-tine PCR分析仔鼠胸腺VDR基因表达三组之间差异无统计学意义(P＞0.05)。Western blot检测VDR蛋白水平的表达显示,低剂量及高剂量组VDR蛋白的表达量均高于对照组,三组之间差异存在统计学意义(P＜0.05)。结论给予母鼠补充维生素D,其仔鼠胸腺VDR蛋白表达量明显增加,与维生素D补充量呈正相关。

关键词: 维生素D, 维生素D受体, 胸腺

Abstract: Objective To detect the changes of vitamin D receptor (VDR) expression in the thymus of filial generation mice after giving pregnant mice different dosages of vitamin D supplementation. Methods Totally 18 female Wistar mice mates with 9 male Wistar mice at 5 pm everyday (the female/male radio was 2∶1).The vaginal secretions of the female mice were collected and made into smears.Conception was recorded after finding sperm in the smears,noting gestational day 0 (GD0).The pregnant mice were separated into three groups:control group,low level of vitamin D supplementation group and high level of vitamin D supplementation group.Different dosages of vitamin D supplementation were given by intramuscular injection from GD 15,with normal saline 0.25 ml/kg,vitamin D 20 μg/kg and vitamin D 100 μg/kg separately for each group.The injection continued until delivery.After 21 days breast-feeding,the thymus of filial generation mice were isolated.The expression of VDRmRNA was detected by Real-time PCR and the expression of VDR protein was detected by Western blot. Results No statistic difference of VDR gene expression was observed among different groups by Real-time PCR(P＞0.05).Western blot showed that the expression of VDR protein in low level of vitamin D supplementation group and high level of vitamin D supplementation group was higher than that of the control group (P＜0.05). Conclusion The expression of VDR in filial generation mice increases if mother mice are given vitamin D supplementation during pregnancy and is positively related to the dosage of vitamin D supplementation.

Key words: vitamin D, vitamin D receptor, thymus

中图分类号:

R179

王志会,赵霞霞,任立红. 孕期鼠补充维生素D对子鼠胸腺维生素D受体表达[J]. 中国儿童保健杂志, 2016, 24(7): 712-714.

WANG Zhi-hui,ZHAO Xia-xia,REN LI-hong.. Effect of vitamin D receptor expression in the thymus of filial generation mice after giving pregnant vitamin D.[J]. journal1, 2016, 24(7): 712-714.

参考文献

[1] Gao L,TAO Y,Zhang L,et al.Vitamin D receptor genetic polymorphisms and tuberculosis:updated system atic review and meta-analysis[J].Int J Tuberc Lung Dis,2010,14(1):15-23.
[2] Perumal N,Mahmud A,Baqui AH,et al.Prenatal vitamin D supplementation and infant vitamin D status in Bangladesh[J].Public Health Nutr,2015,6:1-9.
[3] Pike JW,Meyer MB.The vitamin D receptor:new paradigms for the regulation of gene expression by 1,25-dihydroxyvitamin D3[J].Rheum Dis Clin North Am,2012,38(1):13-27.
[4] Lee SM,Meyer MB,Benkusky NA,et al.Mechanisms of enhancer-mediated hormonal control of vitamin D receptor gene expression in target cells[J].J Biol Chem,2015,290(51):30573-30586.
[5] Wiese RJ,Uhland-Smith A,Ross TK,et al.Up-regulation of the vitamin D receptor in response to 1,25-dihydroxyvitamin D₃ results from ligand-induced stabilization[J].J Biol Chem,1992,267:20082-20086.
[6] Goswami R, Modal AM, Tomar N,et al.Presence of 25(OH)D deficiency and its effect on vitamin D receptor mRNA expression[J].European Journal of Clinical Nutrition,2009,63:446-449.
[7] Agergaard J,Trostrup J,Uth J,et al.Does vitamin-D intake during resistance training improve the skeletal muscle hypertrophic and strength response in young and elderly men?-a randomized controlled trial[J].Nutr Metab(lond),2015,12:32.
[8] Griffin MD,Lutz W,Phan VA,et al.Dendritic cell modulation by 1alpha,25 dihydroxyvitamin D₃ and its analogs:a vitamin D receptor‐dependent pathway that promotes a persistent state of immaturity in vitro and in vivo[J].Proc Natl Acad Sci USA,2001,98(12):6800-6805.
[9] Porto G,Giordano RJ,Marli LC,et al.Identification of novel immunoregulatory molecules in human thymic regulatory CD₄⁺CD₂₅⁺ T cells by phage display[J].PloS One,2011,(8):e21702.
[10] Yu S,Cantorna MT.The vitamin D receptor is required for iNKT cell development[J].Proc Natl Acad Sci USA,2008,105:5207-5212.
[11] Hu Z,Lancaster JN,Ehrlich LI.The contribution of chemokines and migration to the induction of central tolerance in the thymus[J].Front Immunol,2015,6:398.
[12] Lyakh LA,Sanford M,Chekol S,et al.TGF-beta and vitamin D₃ utilize distinct pathways to suppress IL-12 production and modulate rapid differentiation of human monocytes into CD₈₃⁺ dendritic cells[J].J Immunol,2005,174(4),2061-2070.
[13] Heikkinen S,Visnen S,Pehkonen P.Nuclear hormone 1α,25-dihydroxyvitamin D₃ elicits a genome-wide shift in the locations of VDR chromatin occupancy[J].Nucleic Acids Res,2001,39(21):9181-9193.

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孕期鼠补充维生素D对子鼠胸腺维生素D受体表达

Effect of vitamin D receptor expression in the thymus of filial generation mice after giving pregnant vitamin D.

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