先天性铅暴露对发育期子代大鼠体格指标及矿物质营养状态的影响

doi:10.11852/zgetbjzz2017-25-07-10

中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (7): 683-686.DOI: 10.11852/zgetbjzz2017-25-07-10

先天性铅暴露对发育期子代大鼠体格指标及矿物质营养状态的影响

周戬平¹, 王帆², 钟波¹, 易晓青¹, 王雪莹¹, 王晶晶¹, 姜永生¹

1 西安交通大学医学院第二附属医院儿科,陕西西安 710004;
2 陕西省人民医院妇产科,陕西西安 710068

收稿日期:2016-09-20 发布日期:2017-07-10 出版日期:2017-07-10
作者简介:周戬平(1974-),男,陕西人,副主任医师,医学博士,主要从事胚胎期理化损伤与子代健康以及未成熟脑发育与损伤干预方面的研究。

Effects of congenital lead exposure on growth indicators and mineral nutrition status in developmental offspring of rats

ZHOU Jian-ping¹, WANG Fan², ZHONG Bo¹, YI Xiao-qing¹, WANG Xue-ying¹, WANG Jing-jing¹, JIANG Yong-sheng¹

1 Department of Pediatrics,the Second Affiliated Hospital of Medical College of Xi'an Jiaotong University,Xi'an,Shaanxi 710004,China;
2 Department of Obstetrics and Gynecology, the People's Hospital of Shaanxi Province,Xi'an,Shaanxi 710068,China

Received:2016-09-20 Online:2017-07-10 Published:2017-07-10
Supported by:
陕西省科技攻关项目(2016SF-263); 陕西省人民医院孵化基金(2010)

摘要/Abstract

摘要： 目的观察先天性铅暴露对发育期子代大鼠体格发育指标及矿物质水平的影响,分析其损害的特点并初步探讨可能的影响因素。方法选择遗传背景近似的孕鼠自由饮用0.1%及0.2%醋酸铅溶液的方法建立先天性铅暴露模型,其子代分别为低剂量铅暴露组(LG)及高剂量铅暴露组(HG),同时设立正常对照组(NG)。测量仔鼠生后第1~7天(1~7 d)及14 d的体质量及尾长。36 d及60 d仔鼠应用原子吸收法检测血铅、锌、钙及铁水平。60 d仔鼠应用放射免疫法检测血清生长激素(GH)水平。结果先天性铅暴露组仔鼠出生时平均体质量与尾长降低(P<0.01)。2~14 d,LG、HG组仔鼠体质量及尾长增长较NG组显著减缓(P<0.01)。NG、LG、HG组36 d及60 d仔鼠血锌、铁水平差异有统计学意义(P<0.01)。36 d时,NG[(2.03±0.22) mmol/L]、LG[(1.75±0.24) mmol/L]、HG[(1.62±0.21) mmol/L]组仔鼠血钙水平差异有统计学意义(P<0.01)。NG、LG、HG组60 d仔鼠血钙分别为(2.02±0.21)、(1.85±0.22)mmol/L及(1.76±0.17)mmol/L(P<0.01)。NG、LG、HG组60 d仔鼠血清GH分别为:(2.15±0.35)、(1.98±0.24)、(1.86±0.25) ng/ml(P<0.01);HG与NG组(P<0.01),LG与NG组(P<0.01)以及HG与LG组间差异有统计学意义(P<0.05)。结论先天性铅暴露可导致胎鼠宫内发育迟滞及仔鼠体格发育落后,可能与锌、铁、钙等矿物质营养水平降低及GH分泌不足有关。

关键词: 铅暴露, 体格生长, 发育迟滞, 胚胎期, 生长激素

Abstract: Objective To explore the effects of congenital lead exposure on physique indicators and mineral nutrition status in developmental offspring of rats. Methods The animal model of congenital lead exposure was established with the use of similar genetic background Sprague Dawley pregnant rats drinking freely 0.1% (low-dose group,LG) and 0.2% (high-dose group,HG) lead acetate solution.The normal control group (NG) was also established.Dynamic measurement of the weights and the tail lengths were adopted to show the physical growth conditions of the offspring rats on 1 to 7 days (1 to 7 d) and 14 days (14 d) after birth.The levels of plasma lead,zinc,iron,and calcium on 36 d and 60 d,serum growth hormone (GH) were measured through the Methods of atomic absorption spectrometry and radioimmunoassay. Results The average birth weight and tail length of offspring rats decreased in congenital lead exposure groups (P<0.01).Compared to group NG,the growth of weight and tail length on 2 to 14 d was significantly slow in groups LG and HG (P<0.01).As for the levels of plasma zinc and iron,there were significant statistical differences among groups NG,LG and HG on both 36 d and 60 d (P<0.01).On 36 d,the offspring rats' plasma calcium in groups NG[(2.03±0.22) mmol/L],LG[(1.75±0.24) mmol/L] and HG[(1.62±0.21) mmol/L] were significantly different (P<0.01).On 60 d,the plasma calcium levels of groups NG,LG and HG were respectively (2.02±0.21)mmol/L,(1.85±0.22)mmol/L and (1.76±0.17)mmol/L (P<0.01).The serum GH of 60 d offspring rats were (2.15±0.35) ng/ml for NG,(1.98±0.24) ng/ml for LG,and (1.86±0.25) ng/ml for HG (P<0.01).There were also significant statistical differences between HG and NG (P<0.01),LG and NG (P<0.01) or HG and LG (P<0.05). Conclusion Congenital lead exposure can lead to intrauterine growth restriction in fetal rats and physique growth delay in offspring rats after birth,which may be related to decreases of GH secretion and deficiency of mineral nutrient zinc,iron and calcium.

Key words: lead exposure, physique growth, development retardation, embryonic period, growth hormone

中图分类号:

R179

周戬平, 王帆, 钟波, 易晓青, 王雪莹, 王晶晶, 姜永生. 先天性铅暴露对发育期子代大鼠体格指标及矿物质营养状态的影响[J]. 中国儿童保健杂志, 2017, 25(7): 683-686.

ZHOU Jian-ping, WANG Fan, ZHONG Bo, YI Xiao-qing, WANG Xue-ying, WANG Jing-jing, JIANG Yong-sheng. Effects of congenital lead exposure on growth indicators and mineral nutrition status in developmental offspring of rats[J]. journal1, 2017, 25(7): 683-686.

参考文献

[1] Flora G,Gupta D,Tiwari A.Toxicity of lead:A review with recent updates[J].Interdiscip Toxicol,2012,5(2):47-58.
[2] Ji A,Wang F,Luo W,et al.Lead poisoning in China:a nightmare from industrialization[J].Lancet,2011,377(9776):1474-1476.
[3] Cohen JE,Amon JJ.Lead poisoning in China:a health and human rights crisis[J].Health Hum Rights,2012,14(2):74-86.
[4] Liu KS,Hao JH,Zeng Y,et al.Neurotoxicity and biomarkers of lead exposure:a review[J].Chin Med Sci J,2013,28(3):178-188.
[5] Badawoud MH,Hassan SM.Effects of exposure to lead on the peripheral motor system of the rat.An ultrastructural study[J].Neurosciences,2013,18(1):52-57.
[6] Al-Saleh I,Shinwari N,Mashhour A,et al.Heavy metals (lead,cadmium and mercury) in maternal,cord blood and placenta of healthy women[J].Int J Hyg Environ Health,2011,214(2):79-101.
[7] 宋波,武柏林,刘士鹏,等.母鼠孕前低水平铅暴露对子代骨骼钙代谢的影响[J].环境与职业医学,2015,32(2):151-154.
[8] 周戬平,王帆,杨玉凤,等.孕期铅暴露对子代大鼠情感行为及学习记忆变化的实验研究[J].中国儿童保健杂志,2012,20(2):135-138.
[9] Sharma D,Shastri S,Sharma P.Intrauterine growth restriction:antenatal and postnatal aspects[J].Clin Med Insights Pediatr,2016,10:67-83.
[10] Bansal S,Deka D,Dhadwal V,et al.Doppler changes as the earliest parameter in fetal surveillance to detect fetal compromise in intrauterine growth-restricted fetuses[J].Srp Arh Celok Lek,2016,144(1-2):69-73.
[11] 徐晓丽,邓军霞,张国成,等.铅中毒儿童各年龄期血锌、钙、铁分析[J].中国儿童保健杂志,2013,21(11):1212-1214.
[12] 陈忠,张斌,覃凌智,等.儿童铅中毒饮食高危因素分析[J].中国儿童保健杂志,2015,23(6):660-663.
[13] 旷满华,杨青廷,翦耀文,等.儿童缺铁性贫血与血铅水平关系的Meta 分析[J].中华疾病控制杂志,2016,20(1):79-83.
[14] Shah F,Kazi TG,Afridi HI.Environmental exposure of lead and iron deficit anemia in children age 1-5 years:a cross sectional study[J].Sci Total Environ,2010,408(22):5325-5330.
[15] Zentner LE,Rondo PH,Duran MC,et al.Relationships of blood lead to calcium,iron,and vitamin C intakes in Brazilian pregnant women[J].Clinical Nutrition,2008,27(1):100-104.
[16] 曹燕花,王东,张阳,等.纳米硫化铅对大鼠学习记忆能力及海马组织中铜锌含量的影响[J].环境与健康杂志,2015,32(7):602-605.
[17] 罗丹,胡蕾,李平,等.铅暴露对雄性仔鼠海马锌离子转运体3 表达的影响[J].环境与健康杂志,2016,33(1):15-18.
[18] 张璟,王文军,李晶,等.铁、锌、铅对学龄儿童生长激素分泌的影响[J].热带医学杂志,2009,9(9):1064-1066.
[19] 杨兴林,王义光,朱武军,等.贵阳市中学生血铅和生长激素水平调查及相关性研究[J].贵州医药,2008,32(12):1129-1131.

先天性铅暴露对发育期子代大鼠体格指标及矿物质营养状态的影响

Effects of congenital lead exposure on growth indicators and mineral nutrition status in developmental offspring of rats

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	王晶, 项思嘉, 鲁莹, 杨蕾, 胡小沙, 钱君, 池霞, 张敏. 早产儿3周岁体格生长及体成分分析[J]. 中国儿童保健杂志, 2023, 31(4): 457-460.
[2]	邓岚柳, 陈立. 药物治疗对注意缺陷多动障碍患儿体格生长影响的研究进展[J]. 中国儿童保健杂志, 2022, 30(8): 874-878.
[3]	程波利, 孙群英, 肖梦, 李星慧, 张会, 杨蓉. 贫血及小红细胞对婴幼儿体格和神经心理发育的影响[J]. 中国儿童保健杂志, 2022, 30(5): 495-499.
[4]	马天贺, 文香淑. GPT2基因突变致常染色体隐性遗传性智力低下49型1例并文献复习[J]. 中国儿童保健杂志, 2022, 30(11): 1267-1271.
[5]	杨玉兰, 孙宾宾, 屈小莉, 江雯. 早产儿矫正24月龄内体格发育及神经心理发育随访分析[J]. 中国儿童保健杂志, 2022, 30(10): 1123-1127.
[6]	易晓青, 韩田田, 刘红红, 王思思, 张蓉, 王爱荣, 肖延风. 维生素D和骨形态发生蛋白-2水平与生长激素缺乏症儿童成骨细胞功能的相关性研究[J]. 中国儿童保健杂志, 2021, 29(7): 701-704.
[7]	张甜, 孙海玲, 张梅, 班博, 孙冰. 儿童营养不足与生长激素/胰岛素样生长因子-1相关性研究进展[J]. 中国儿童保健杂志, 2021, 29(7): 734-737.
[8]	万乃君, 张田, 张金, 孙慧慧, 商然, 师伟佳. 长效重组人生长激素治疗儿童生长激素缺乏症的有效性及安全性研究[J]. 中国儿童保健杂志, 2021, 29(7): 755-758.
[9]	武华红, 李辉, 宗心南, 张亚钦. 中国九城市学龄前儿童饮食行为与体格生长的关系研究[J]. 中国儿童保健杂志, 2021, 29(6): 589-593.
[10]	杨婷婷, 王佩琪, 赵玲, 刘晓静. Temple综合征1例并文献复习[J]. 中国儿童保健杂志, 2021, 29(6): 694-696.
[11]	兰红艳, 陈津津. 小于胎龄儿生长发育的研究进展[J]. 中国儿童保健杂志, 2021, 29(4): 405-408.
[12]	刘慧燕, 林穗方, 李伟栋, 文羽祺, 肖婉琪, 宋燕燕. 广州市社区足月小样儿 0~2岁体格发育的纵向研究[J]. 中国儿童保健杂志, 2021, 29(10): 1068-1071.
[13]	曾佩佩, 黄丽萍, 邓梁琼, 冯玉山, 熊莉, 曾婷, 李红辉. 重组人生长激素治疗对特发性矮小症儿童心理状态影响的分析[J]. 中国儿童保健杂志, 2021, 29(1): 71-74.
[14]	祝丹, 余文, 徐健. 腕骨发育评价在青春期前儿童生长激素缺乏症诊断中的参考作用[J]. 中国儿童保健杂志, 2020, 28(7): 733-736.
[15]	刘瑛, 刘翠, 宁静, 郭勇, 吴婕翎. 基于不同体重指数的青春前期生长激素缺乏症患儿采用重组人生长激素治疗的疗效分析[J]. 中国儿童保健杂志, 2020, 28(4): 385-388.