关键词: 过敏性哮喘, microRNA-29c, CD4+辅助性T细胞1 / CD4+辅助性T细胞2, 外周血, 单个核细胞

Abstract: Objective To analyze the expression of microRNA 29c (mir-29c) in peripheral blood mononuclear cells of children with allergic asthma (AA) and its correlation with the balance of CD4+helper T cell 1/CD4+helper T cell 2 (Th1/Th2).Methods From October 2015 to December 2018,Totally 94 children with AA were divided into stable group (32 cases),mild acute attack group (27 cases) and moderate acute attack group (35 cases),and another 40 healthy children underwent physical examination were selected as the control group.Peripheral blood was collected from all subjects,serum and peripheral blood mononuclear cell (PBMC) were separated,the levels of mir-29c,Th1/Th2,IFN-gamma,IL-4,T-box transcription factor (T-bet) and GATA binding protein 3 (GATA3) were detected.Results Compared with the control group,the levels of mir-29c,Th2 subgroup,GATA3 protein in PBMC and the level of IL-4 in serum of children with AA in stable group,mild and moderate acute attack group increased in turn,while the percentage of Th1 subgroup,Th1/Th2,T-bet protein level,T-bet/GATA3,serum IFN-γ level and IFN-γ/IL-4 in PBMC decreased in turn,and there was significant difference between any two groups (P<0.05).The levels of mir-29c in peripheral blood of children with AA were negatively correlated with Th1/Th2,IFN-γ/IL-4 and T-bet/GATA3 (r=-0.823,-0.789,-0.804,P<0.05).Conclusion Mir-29c is up-regulated in peripheral blood mononuclear cells of children with AA and may affect the occurrence of AA through Th1/Th2 balance.

Key words: allergic asthma, microRNA-29c, CD4+helper T cell 1/CD4+helper T cell 2, peripheral blood, mononuclear cells