烟酰胺核糖对母婴隔离后青春期抑郁大鼠的作用研究

doi:10.12092/j.issn.1009-2501.2018.09.008

中国临床药理学与治疗学 ›› 2018, Vol. 23 ›› Issue (9): 1015-1021.doi: 10.12092/j.issn.1009-2501.2018.09.008

烟酰胺核糖对母婴隔离后青春期抑郁大鼠的作用研究

王柔昕，方泽康，姚思琦，薛迎迎，赵叶颖，叶夷露

杭州医学院，杭州 310053，浙江

收稿日期:2018-05-15 修回日期:2018-07-20 出版日期:2018-09-26 发布日期:2018-09-26
通讯作者: 叶夷露，女，博士，教授，研究方向：神经精神药理学。 Tel：0571-87692673 E-mail：yeyilu@hmc.edu.cn
作者简介:王柔昕，女，本科，研究方向：神经精神药理学。 E-mail：1036745669@qq.com
基金资助:
浙江省科技厅科研项目（2016C37099）；浙江省医药卫生科技项目（2018239491）；浙江省大学生科技创新计划（新苗计划）项目（2017R436001）

Effects of nicotinamide ribose on adolescent depression rats after neonatal maternal deprivation

WANG Rouxin, FANG Zekang, YAO Siqi, XUE Yingying, ZHAO Yeying, YE Yilu

Hangzhou Medical College, Hangzhou 310053, Zhejiang, China

Received:2018-05-15 Revised:2018-07-20 Online:2018-09-26 Published:2018-09-26

摘要/Abstract

摘要：

目的: 研究烟酰胺核糖对母婴隔离后青春期抑郁大鼠的作用。方法: 母婴隔离大鼠从出生后第2天起每日与母鼠隔离3 h，持续到出生后第21天。出生后第21天，隔离组大鼠随机分为抑郁模型组（生理盐水，n=6）、舍曲林组（40 mg·kg^-1·d^-1，n=7）和烟酰胺核糖组（400 mg·kg^-1·d^-1，n=7），非隔离组大鼠给予生理盐水溶液（n=6），每日一次，持续到出生后第42天。第42天起，采用强迫游泳实验、糖水偏好实验、开场实验、新物体识别实验进行行为学检测。冰上断头取脑后，迅速分离海马和前额皮层，采用ELISA试剂盒分别检测前额皮层和海马区5-羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NE）、烟酰胺腺嘌呤二核苷酸（NAD⁺）含量。结果: 母婴隔离大鼠体质量增长缓慢，烟酰胺核糖对抑郁大鼠体质量无改善作用；烟酰胺核糖使糖水消耗率增加、不动时间缩短、站立次数增加、分辨率和分辨指数提高；烟酰胺核糖增加前额皮层DA、NE、NAD⁺含量，增加海马区DA、NAD⁺含量。结论: 烟酰胺核糖通过提高神经递质DA和NAD⁺含量，改善抑郁样行为，从而为抗抑郁药物的筛选提供实验基础。

关键词: 烟酰胺核糖, 抑郁, 母婴隔离, 青春期, 大鼠

Abstract:

AIM: To explore the effects of nicotinamide ribose (NR) on adolescent depression rats after neonatal maternal deprivation (NMD). METHODS: NMD was performed as mother-infant separation from postnatal day (PND) 2 to PND 21 for 3 hours (9∶00-12∶00 AM) each day. At PND 21, isolated rats were divided into three groups randomly: isolated depression group (saline, n=6), sertraline group (40 mg·kg^-1·d^-1, n=7) and NR group (400 mg·kg^-1·d^-1, n=7), which were treated for 21 days and once daily. The normal control rats were treated with saline (n=6). From PND 42 on, forced swimming test (FST), sucrose preference test (SPT), open field test (OFT) and novel object recognition test (NORT) were detected. Then the contents of 5-HT, DA, NE and NAD⁺ in the hippocampus and prefrontal cortex (PFC) were detected by respective ELISA kits. RESULTS: Exposed to NMD, the body weight increased slightly. NR treatment had no improvement on body weight compared with normal control group. However, NR treatment increased the sucrose preference ratio, rearing times, discrimination ratio (DR) and discrimination index (DI). Also NR treatment decreased the immobility time in FST. The results of ELISA showed NR treatment increased the contents of DA, NE, NAD⁺ in the frontal cortex and the contents of DA, NAD⁺in hippocampus. CONCLUSION: NR improved the depressive behaviors through the increase of neurotransmitters contents and NAD⁺ . It maybe provide experimental basis for screening of anti-depressants.

Key words: nicotinamide ribose (NR), depression, neonatal maternal deprivation (NMD), adolescent, rats

中图分类号:

王柔昕，方泽康，姚思琦，薛迎迎，赵叶颖，叶夷露. 烟酰胺核糖对母婴隔离后青春期抑郁大鼠的作用研究[J]. 中国临床药理学与治疗学, 2018, 23(9): 1015-1021.

WANG Rouxin, FANG Zekang, YAO Siqi, XUE Yingying, ZHAO Yeying, YE Yilu. Effects of nicotinamide ribose on adolescent depression rats after neonatal maternal deprivation[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(9): 1015-1021.

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烟酰胺核糖对母婴隔离后青春期抑郁大鼠的作用研究

Effects of nicotinamide ribose on adolescent depression rats after neonatal maternal deprivation

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