关键词: 眼镜蛇毒, 凝胶剂, 镇痛, 抗炎

Abstract:

AIM: To explore the effect of topical analgesia of cobra venom analgesic factor (CVAF) in southern Anhui province and its possible mechanism. METHODS: Male SD rats were divided into normal saline control group (NS group), complete Freund's adjuvant pain group (CFA group), diclofenac sodium analgesic positive control group (CFA+SL group), flurbiprofen analgesic positive control group (CFA+FB group), CVAF analgesia group (CFA+CVAF group). The analgesic effect of CVAF gel, the active component of cobra venom was observed and compared. The pain thresholds of rats in each group were measured with a thermal tingling tester and mechanical tenderness tester. The content of IL-1 and TNF-α in the homogenate of spinal cord in each group was detected by using ELISA, the pathological changes of rat toe tissue was observed by using HE staining.RESULTS:Compared with CFA group, the weight loss of CVAF analgesia rats on the 7th and 14th day after administration was improved (P<0.05, P<0.01). The mechanical pain threshold and the thermal pain threshold of the CVAF group were all improved (P<0.01). The concentrations of IL-1 and TNF-α in the homogenate of spinal cord were relatively low (P<0.01). In HE staining, the toe tissue edema in the CVAF group was significantly reduced, and the inflammatory infiltration was relatively insignificant. CONCLUSION: The CVAF gel, the active ingredient of Cobra venom in southern Anhui province, can improve the inflammatory pain in rats hot pain threshold and mechanical pressure threshold; its mechanism may be related to reduce the release of inflammatory mediators, inhibiting the inflammatory response.

Key words: cobra venom, gel preparation, analgesia, anti-inflammatory