miR-423-5p靶向水通道蛋白1对雨蛙肽诱导的急性胰腺炎AR42J细胞损伤的影响

doi:10.12092/j.issn.1009-2501.2019.12.012

中国临床药理学与治疗学 ›› 2019, Vol. 24 ›› Issue (12): 1394-1401.doi: 10.12092/j.issn.1009-2501.2019.12.012

miR-423-5p靶向水通道蛋白1对雨蛙肽诱导的急性胰腺炎AR42J细胞损伤的影响

廖伟¹，汤玉成¹，周婷婷¹，周芙容²

¹重庆市中医院普外科, 重庆 400021；²重庆市精神卫生中心, 重庆 401147

收稿日期:2019-05-07 修回日期:2019-07-05 出版日期:2019-12-26 发布日期:2020-01-07
通讯作者: 汤玉成，男，硕士，医师，主要从事肝胆及胃肠临床研究。 Tel:15213489737 E-mail：422896233@qq.com
作者简介:汤玉成，男，硕士，医师，主要从事肝胆及胃肠临床研究。 Tel:15213489737 E-mail：422896233@qq.com
基金资助:
重庆市科学技术委员会绩效激励引导专项课题（2018-cj-25）

Effect of miR-423-5p on the injury of AR42J cells induced by caerulein through targeting AQP1

LIAO Wei ¹, TANG Yucheng ¹, ZHOU Tingting ¹, ZHOU Furong ²

¹Department of General Surgery, Hospital of Traditional Medicine, Chongqing 400021, China;² Medical Health Center of Chongqing, Chongqing 401147, China

Received:2019-05-07 Revised:2019-07-05 Online:2019-12-26 Published:2020-01-07

摘要/Abstract

摘要：

目的:研究miR-423-5p对雨蛙肽诱导的胰腺AR42J细胞损伤的影响及其分子作用机制。方法:通过雨蛙肽处理AR42J细胞建立胰腺炎损伤模型，qRT-PCR检测AR42J细胞中miR-423-5p的表达，Western blot检测水通道蛋白1（aquaporin 1,AQP1）、Bcl-2、Bax和caspase-3蛋白表达，ELISA法检测培养上清中IL-6和TNF-α的分泌水平，流式细胞术测定细胞凋亡率，双荧光素酶报告系统验证miR-423-5p与AQP1间的调控关系。结果:在雨蛙肽诱导的AR42J细胞中miR-423-5p表达上调，AQP1表达下调；抑制miR-423-5p和过表达AQP1均可减轻雨蛙肽诱导的AR42J细胞损伤，抑制细胞凋亡；双荧光素酶报告系统结果显示miR-423-5p靶向负调控AQP1的表达；抑制AQP1逆转了抑制miR-423-5p对雨蛙肽诱导的胰腺AR42J细胞损伤的作用。结论:miR-423-5p通过靶向AQP1减轻雨蛙肽诱导的胰腺AR42J细胞损伤，抑制细胞凋亡。

关键词: 胰腺炎, 雨蛙肽, miR-423-5p, 水通道蛋白1, AR42J细胞损伤, 凋亡

Abstract:

AIM: To investigate the effect of miR-423-5p on the injury of pancreatic AR42J cells induced by caerulein and explore the underlying molecular mechanism. METHODS: A model of pancreatitis injury was established by treating AR42J cells with caerulein. The expression level of miR-423-5p in AR42J cells was detected by qRT-PCR. The expression levels of aquaporin 1(AQP1), Bcl-2, Bax and capase3 were measured by Western blot. The levels of IL-6 and TNF-α in culture supernatants were detected by enzyme-linked immunosorbent assay (ELISA). The cell apoptotic rate was determined by flow cytometry. The relationship between miR-423-5p and AQP1 was verified by dual-luciferase reporter assay system. RESULTS:miR-423-5p was up-regulated and AQP1 was down-regulated in AR42J cells induced by caerulein. Inhibition of miR-423-5p or overexpression of AQP1 alleviated the injury of AR42J cells induced by caerulein and inhibited cell apoptosis. The results of dual-luciferase reporter assay system suggested that miR-423-5p targeted and negatively regulated the expression of AQP1. Inhibition of AQP1 reversed the effects of inhibition of miR-423-5p on the injury of AR42J cells induced by caerulein. CONCLUSION: miR-423-5p alleviates the injury of pancreatic AR42J cells induced by caerulein and inhibited apoptosis by targeting AQP1.

Key words: pancreatitis, caerulein, miR-423-5p, aquaporin 1, AR42J cell injury, apoptosis

中图分类号:

R965.2

廖伟，汤玉成，周婷婷，周芙容. miR-423-5p靶向水通道蛋白1对雨蛙肽诱导的急性胰腺炎AR42J细胞损伤的影响[J]. 中国临床药理学与治疗学, 2019, 24(12): 1394-1401.

LIAO Wei, TANG Yucheng, ZHOU Tingting, ZHOU Furong. Effect of miR-423-5p on the injury of AR42J cells induced by caerulein through targeting AQP1[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(12): 1394-1401.

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miR-423-5p靶向水通道蛋白1对雨蛙肽诱导的急性胰腺炎AR42J细胞损伤的影响

Effect of miR-423-5p on the injury of AR42J cells induced by caerulein through targeting AQP1

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