关键词: 环孢素A, 表皮细胞, 组织培养, 细胞增殖, 乳大鼠

Abstract: Aim The growth characteristic of cultured epidermal cell and the inhibition effect of cyclosporin A (CSA) on cell proliferation at the doses of l μg/ml, 3 μg/ml and 6 μg/ml were observed. Methods The single epidermal cells of infant mice were cultured without EGF (eidermal growth factor). Growth curve was descriped by numbering the alive cells in different times. The inhibition rate of CSA on cell proliferation was calculated. Results Epidermal cells might be survived for about 15 days without EGF. Cells would attach to the wall of bottle in 24 h. The growth top time was in 5 ~ 7 days. There were some hollows in cytoplasm after 10 days. There was no inhibition effects of CSA on cell proliferation when cells were cultured for 24 h and 48 h at the dose of 1 μg/ml but a weak inhibition effect was seen in 72 h with the inhibition rate being 15% when cells were cultured with 3 μg/ml CSA for 48 h and 72 h the inhibition rates were 18% and 30%. There was no inhibition effect in 24 h still. When cells were cultured with 6 μg/ml for 24 h 48 h and 72 h the inhibition rates were 16%, 40% and 65% respectively. Conclusion CSA inhibit cell proliferation in a dose and time dependent manner.

Key words: cyclosporin A, epidemal, orgnization culture, cell proliferation, infant mice