关键词: 利多卡因, 异丙酚, 细胞膜Na⁺-K⁺-ATP酶, 肌浆网Ca²⁺-ATP, 缺血再灌注/心肌

Abstract: Aim To investigate the effects of lidocaine and propofol on the activities of myocardial sarcolemmal Na⁺-K⁺-ATPase and SR Ca²⁺-ATPase following ischemia-reperfusion. Methods forty-eight rats were randomly divided into six groups:Sham group(the coronary was not sutured), NS group (saline 5 ml·kg^-1), L5 group (lidocaine 5 mg·kg^-1), L₁₀ group (lidocain 10 mg·kg^-1), P300 group (propofol 300 μg·kg^-1·min^-1)and P1000 group (propofol 1000 μg·kg^-1·min^-1).Five minutes before ischemia, Saline or lidocaine was injected intrav eneously within 30 seconds and propofol was infused intraveneously till the onset of ischemia.The left coronary artery was occluded with a silk suture for fifteen minutes, then loosened to achieve reperfusion for ten minutes.The activities of sarcolemmal Na⁺-K⁺-AT Pase and SR Ca²⁺-A TPase were measured with pho to colorme-t re.Results Compared with that in NS group the activ ities of S R Ca²⁺-A TPase in the lido caine groups were increased significantly (P<0.05), while the activites of sarco lemmal Na⁺-K⁺-A TPase in propofol groups were increased significantly(P<0.05, P<0.01 respectively).Propofol of 1000 μg·kg^-1·min^-1 could increase the activity of SR Ca²⁺-ATPase (P<0.05).Lidocaine of 10 ml·kg^-1 could increase the activity of sa rco lemmal Na⁺-K⁺-ATP ase significantly (P<0.05). Conclusion Lido caine, propofol could attenuate my ocardial ischemia-reperfusion injury by increasing the activities of sarcolemmal Na⁺-K⁺-ATPase and SR Ca²⁺-AT Pase during reperfusion.

Key words: lidocaine, propofol, sarcolemma Na⁺-K⁺-AT Pase, sarco plasic reticulum Ca²⁺-ATPase, ischemia-reperfusion/myocardium