关键词: 大鼠, 血管内皮, 损伤, 动物模型

Abstract: Aim To establish a model of the vascular endothelial cell (VEC) injury in SD rats. Methods SD rats were randomly divided into the control and the model groups. The model rats were injected with adrenaline diluted to 2.5 times0.05 mg·100 g^-1(tid) for 5 d continously. From the 4th d, they were irritated for 5 min in the 0°C cold-water in the middle between adrenaline injections. The control rats were given 0.9% NS as above. At 6th d, blood samples were taken from carotid arteries of the rats and the CEC counts, t-PA、PAI activities, 6 keto-PGF1 concentrations and the platelet aggregation rate_(max) were detected respectively.Results In the model group, as compared with those in the control group, t-PA activity and 6-keto-PGF1. concentration decreased significantly(P<0.01,P<0.05),while the CEC count、PAI activity and the platelet aggregation rate_(max) increased obviously (P<0.01).Conclusions A large dose of adrenaline in combination with cold-water itritation can cause the VEC injury in SD rats.

Key words: rats, endothelium, injury, animal model