关键词: 动物模型, 粘连, 联合药物治疗, ROC 曲线, 尿囊素, 甲硝唑, 地塞米松

Abstract: Aim To set up a novel animal model ofintra-abdominal adhesion and tode termine whether the tissue plasminogen activator activity (PAA)in exudate can be taken as an indicator to judge the formation of the adhesion.Methods Rats wererandomly dividedinto 2 groups. Each animal in both groups was opened the abdominal cavity via midline laparo tomy witho ut any disinfectant measures.2-cm section from the cecal end was clamped and lig ated, 1-cm cecum of the section was cut, and another 1-cm end from the ligated site was kept.After the contentin the end was extruded, the cecum was put back without using any antibacterial agent.Beforethe skin closure, an Effective combination AMD (allantoin, metronidazole and dexamethasone in combination), was g iven (ip)according to 1.5 ml per 100 g body weight (60.6 mg·kg^-1).The control group was injected (ip)the samevolumeof normal saline. After 6 h, the exudate was extracted from drainage-tube, with therats varying posture, and after 1 kw, therats werekilled forexamining the intra-abdominal adhesion.The values of PAA of exudate in both groups wereanaly zed by therelative operating characteristic curve (ROC).Results In the control group, all 20 rats formed the adhesions, the amo unt of exudate = (1.25±0.09)ml, the numberof WBC(×103) = (23.1±6.6)mm³ and PAA = (0.9±0.4)IU·ml^-1 in the exudate of abdominal cavity.In AMD group, however, therewas not the adhesion formations (0/20), the amount of exuade was (0.52±0.04)ml (P<0.01), the numberof WBC (×103)(10.6±4.2)mm³ (P<0.01), and PAA (23.1±6.6) IU·ml^-1(P<0.01)in exuade.ROCanalysis indicated thati f PAA >1.24 IU·ml^-1 in the exuade, the adhesion was difficult toform, and vice versa.Conclusion This animal model can be taken as an effective to olto evaluate the human adhesion related to multi-links on the pathog enesis, and the PAA in exudate an indicator to judge intra-abdominal adhesion formation.

Key words: animal model, adhesion, combination drug therapy, ROC curve, allantoin, metronidazole, dexamethasone