侧脑室注射东莨菪碱对吗啡依赖大鼠戒断反应的抑制作用

中国临床药理学与治疗学 ›› 2001, Vol. 6 ›› Issue (4): 326-329.

侧脑室注射东莨菪碱对吗啡依赖大鼠戒断反应的抑制作用

顾钧, 唐甩恩, 周文华, 杨国栋

宁波市微循环与莨菪类药研究所, 宁波戒毒研究中心, 宁波 315010

收稿日期:2001-04-28 修回日期:2001-06-15 出版日期:2001-08-26 发布日期:2020-11-30

Intracerebroventricular administration of scopolamine attenuates naloxone precipitated morphine abstinence syndrome

GU Jun, TANG Shuai-En, ZHOU Wen-Hua, YANG Guo-Dong

Ningbo Institute of Microcirculation and Henbane, Ningbo Drug Addiction and Treatment Center, Ningbo 315010

Received:2001-04-28 Revised:2001-06-15 Online:2001-08-26 Published:2020-11-30
About author:GU Jun, male, master degree in medicine, engaged in research of drug addiction.YANG Guo-Dong, male, senior researcher, engaged in research of drug addiction and treatment.

摘要/Abstract

摘要： 目的观察非选择性毒蕈碱(M)受体拮抗剂东莨菪碱、选择性M1 受体拮抗剂哌拉唑嗪以及选择性M2受体拮抗剂美索四氨对吗啡戒断反应的影响。方法采用吗啡依赖大鼠模型侧脑室注射上述药物, 并用腹腔注射纳洛酮诱发戒断反应, 记录60 min 内戒断症状。结果侧脑室注射东莨菪碱(25, 50 μg)、哌拉唑嗪(20 μg)和美索四氨(25 μg)可明显抑制由纳洛酮诱发戒断反应, 东莨菪碱减轻吗啡戒断症状呈明显量效关系。结论侧脑室注射东莨菪碱能减轻吗啡戒断反应, 提示中枢胆碱能神经毒蕈碱(M)受体在吗啡依赖和耐受过程中起重要作用。

关键词: 东莨菪碱, 哌拉唑嗪, 美索四氨, 吗啡戒断, 毒蕈碱受体

Abstract: Aim To investigate the effect of cholinergic receptors in central nervous system on morphine dependent rats.Methods Male Sprague-Dawley rats w eighing 200～250 g were rendered dependent on morphine by subcutaneous injection of mo rphine in increasing doses for 5 days.Prior to testing, rats received infusion of either asolution of drug (scopolamine, pirenzepine, methoctramine)or vehicle (normal saline)in lateral cerebral vent ricle.Withdrawal behavior was monitored for 60 min after int raperitoneal administ ration of naloxone.Results The animal pretreatment with scopolamine (25, 50 μg)or muscarinic subtype antagonists (20 μg pi renzepine, 25 μg methoct ramine) could significantly at tenuate naloxone precipitated morphine abstinence syndrome.Cent ral administ ration of scopolamine could reduce reaction of morphine withdrawal in a dose-dependent manner.Conclusion The central cholinergic receptors may be play an impo rtant role in the process of morphine withdraw al. Both M1 and M2 receptors may cont ribute to the development of morphine dependence and tolerance.

Key words: scopolamine, pirenzepine, methoctramine, morphine withdrawal, muscarinic receptor

中图分类号:

R996

顾钧, 唐甩恩, 周文华, 杨国栋. 侧脑室注射东莨菪碱对吗啡依赖大鼠戒断反应的抑制作用[J]. 中国临床药理学与治疗学, 2001, 6(4): 326-329.

GU Jun, TANG Shuai-En, ZHOU Wen-Hua, YANG Guo-Dong. Intracerebroventricular administration of scopolamine attenuates naloxone precipitated morphine abstinence syndrome[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2001, 6(4): 326-329.

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