欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (2): 110-114.

• 基础研究 • 上一篇    下一篇

螺旋藻多糖对小鼠移植性肿瘤化疗后造血恢复及相关细胞因子的影响

张洪泉, 刘晓梅   

  1. 扬州大学医药研究所, 扬州 225001
  • 收稿日期:2002-01-14 接受日期:2002-02-27 出版日期:2002-04-26 发布日期:2020-11-27

Effect of polysaccharide from spirulina platensis on hematopoietic recovery and related cytokines in mice with transplant tumor after treated by chemotherapy

ZHANG Hong-Quan, LIU Xiao-Mei   

  1. Medical and Pharmacological Institute of YangzhouUniversity, Yangzhou 225001
  • Received:2002-01-14 Accepted:2002-02-27 Online:2002-04-26 Published:2020-11-27
  • Contact: ZHANG Hong-Quan, correspondence author, male, professor.Tel:86-514-797-8877 Fax:86-514-734-1733 E-mail:zhanghqmd@x263.net
  • Supported by:
    1 The Project Supported by Nature Science Foundation of Jiangsu Province,NoBJ98313.

摘要: 目的: 研究螺旋藻多糖(polysaccharide fromspirulina platensis, PSP) 对小鼠移植性肿瘤化疗后造血恢复及相关细胞因子的影响。方法: 通过右前肢皮下接种肿瘤细胞形成小鼠移植性实体瘤, 给予PSP 连续灌胃 10 d, d 4 给予环磷酰胺(CTX) 腹腔注射连续 3 d。于 d 11, 分别观察了外周血细胞、骨髓有核细胞及 CFU-S 计数, 用紫外分光光度计检测骨髓DNA 含量, 用双抗体夹心 ELISA 法检测血清中IL-1、IL-3、GM-CSF、TNF 含量。 结果: CTX 可造成明显骨髓抑制, 而 PSP 则提升了外周血细胞、增加了骨髓中有核细胞计数和 DNA 含量以及促进了 CFU-S形成。 此外, PSP 还增加了血清中 IL-1、IL-3、GM-CSF、TNFα含量。结论: PSP 可能通过促进内源性细胞因子的分泌来实现其促进小鼠移植性肿瘤化疗损伤后的造血恢复。

关键词: 螺旋藻多糖, 移植性肿瘤, 骨髓抑制, 造血恢复, 细胞因子

Abstract: AIM: To evaluate the effect of polysac-charide from spirulina platensis (PSP) on hematopoietic recovery and related cytokines in mice with transplant tu-mor after treated by chemotherapy.METHODS: The tu-mor cells were subcutaneously injected in the right forefoot to induce transplant solid tumor in mice.The mice were ig with PSP for 10 d and injected intraperitoneally with cyclophosphamide (CTX) for 3 d.On d 11, the count of peripheral blood cell, nucleated cell in bone marrow and CFU-S (Colony Forming Unit-Spleen) was observed;The content of DNA in bone marrow was inspected by UV-spectrophotometer;The cytokines in serumswere inspect-ed by double antibody sandwich ELISA.RESULTS: CTX could induce evident myelosuppression.But PSP could elevate the level of peripheral blood cell, increase nucleated cell and DNA in bone marrow, and promote CFU-S formation.In addition, PSP could also increase the content of IL-1, IL-3, GM-CSF, and TNF-αin serum.CONCLUSION: It is probably that PSP acceler-ates the hematopoietic recovery in mice with transplant tu-mor treated by chemotherapy by promoting endogenous cy-tokines secretion.

Key words: polysaccharide, spirulina platensis, transplant tumor, myelosuppression, hematopoietic recov-ery, cytokines

中图分类号: