亚氨乙基赖氨酸对庆大霉素所致豚鼠肾脏损伤的拮抗作用

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (3): 205-208.

亚氨乙基赖氨酸对庆大霉素所致豚鼠肾脏损伤的拮抗作用

何青莲, 谭敏, 石灵春, 张维彬

广州中医药大学第二附属医院病理科, 广州 510120

收稿日期:2001-12-24 修回日期:2002-03-25 发布日期:2020-12-01
作者简介:何青莲, 联系作者, 女, 主治医师。Tel:020-81887233-343E-mail:entxm@hotmail.com

Anti-toxical effect of iminoethyl-lysine on guinea pig renal damaged by gentamicin

HE Qing-Lian, TAN Min, SHI Lin-Qun, ZHANG Wei-Bin

Department of Pathology, the Second Hospital, Guangzhou Traditional Chinese Medicine University, Guangzhou 510120

Received:2001-12-24 Revised:2002-03-25 Published:2020-12-01

摘要/Abstract

摘要： 目的: 探讨诱生型一氧化氮合成酶抑制剂(induciblenitricoxide synthase, iNOS) 亚氨乙基赖氨酸(Imi) 对庆大霉素(Gen) 所致豚鼠肾脏损伤的拮抗作用。方法: 实验动物分4 组:正常动物组、Gen 组、Gen+Imi 组和Imi 组。Gen 组和Gen +Imi 组动物皮下均注射庆大霉素100 mg·kg^-1·d^-1 日, 连续10 d, 同时Gen +Imi 组腹腔注射Imi 10 mg·kg^-1·d^-1, Gen 组腹腔注射等量生理盐水;正常动物组皮下与腹腔均注射等量的生理盐水。Imi 组腹腔注射与Gen +Imi 组等量Imi 。所有动物在实验前后均行肾功能检查。所有各组肾脏均行石蜡包埋、切片、HE 染色、光镜观察;同时行iNOS 免疫组织化学染色。结果: iNOS 在正常动物组和Imi 组肾脏呈阴性表达, 在Gen +Imi 组和Gen组呈阳性表达, 且Gen 组iNOS 活性明显强于Gen +Imi 组;Gen 组肾脏损伤明显重于Gen +Imi 组。结论: iNOS 在庆大霉素所致豚鼠肾脏损伤中呈阳性表达,Imi 显著抑制iNOS 活性, 且对肾脏损伤有明显拮抗作用, 提示一氧化氮在庆大霉素所致豚鼠肾脏损伤病理过程中起重要作用。

关键词: 肾脏, 一氧化氮合成酶, 庆大霉素, 免疫组织化学

Abstract: AIM: To investigate the anti-toxic effect of iminoethyl-lysine (Imi), an inhibitor of inducible nitricoxide synthase (iNOS), on the guinea pig renal injury with gentamicin (Gen).METHODS: The experiment was consisted offour groups:normal animal group, gentamicin model group (Gen group), Gen +Imi group and Imi group.Guinea pigs of both Gen group and Gen +Imi group were given (sc) Gen 100mg·kg^-1·d^-1 for 10 d, and the guinea pigs of Gen +Imi group were treated with Imi intraperitoneally.Gen group were treated with the samevolumeof saline.Guinea pigs of normal animal group were treated with subcutaneous and venous injection of the samevolumeof saline.Guinea pigs of Imi group were treated with the samedose of Imi.The renal function of guinea pigs of all groups was examined before and after experiment.Renals of all groups were embedded with paraffin, sectioned, stained with HE, and examined with the light microscope.iNOS was examinedin the kedney of all groups with immunohistochemistry.RESULTS: The expression of iNOS was negative in kidney of normal animal group and Imi group, and positive in both Gen group and Gen +Imi group.The activity of iNOS of Gen group was significantly stronger than that of Gen +Imi group.The damage of renal of Gen group was much more severely than that of Gen +Imi group.CONCLUSION: The expression of iNOS is positive in the renal damaged with Gen.Imi can significantly inhibit the activity of iNOS, and has also the significant anti-toxical effect on the renals damaged with Gen.It suggests that nitricoxide takes part in the process of pathology of renals damaged by gentamicin.

Key words: renal, nitricoxide synthase, Gen, immunohistochemistry

中图分类号:

R965.2

何青莲, 谭敏, 石灵春, 张维彬. 亚氨乙基赖氨酸对庆大霉素所致豚鼠肾脏损伤的拮抗作用[J]. 中国临床药理学与治疗学, 2002, 7(3): 205-208.

HE Qing-Lian, TAN Min, SHI Lin-Qun, ZHANG Wei-Bin. Anti-toxical effect of iminoethyl-lysine on guinea pig renal damaged by gentamicin[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(3): 205-208.

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