抗氧化作用可能是人参皂甙Rg1 抗细胞凋亡的机制1

中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (5): 412-416.

抗氧化作用可能是人参皂甙Rg1 抗细胞凋亡的机制¹

方芳, 陈晓春, 朱元贵

福建医科大学附属协和医院, 福建省老年医学研究所, 福州 350001

收稿日期:2002-04-05 修回日期:2002-05-06 发布日期:2020-11-26
通讯作者: 陈晓春, 男, 教授, 博士生导师。Tel:0591-3377664 Email:xcchen329@sohu.com
作者简介:方芳, 女, 主治医师, 医学硕士。
基金资助:
1 福建省自然科学基金资助项目(№C0110015)

Possible mechanism of mitochondria on anti-apoptotic effect of ginsenoside Rg1 on MPP⁺-induced cellular apoptosis

FANG Fang, CHEN Xiao-Chun, ZHU Yuan-Gui

The Affiliated Union Hospital of Fujian Medical University, and Fujian Provincial Institute of Geriatrics, Fuzhou 350001

Received:2002-04-05 Revised:2002-05-06 Published:2020-11-26

摘要/Abstract

摘要： 目的探讨人参皂甙Rg1 对MPP⁺诱导SHSY5Y 细胞凋亡的保护作用及其可能的机制。方法用吖啶橙溴化乙锭染色观察SHSY5Y 细胞凋亡率, 流式细胞仪检测线粒体跨膜电位及细胞内活性氧(ROS) 水平, Western Blot 法检测bcl-2、bax 蛋白表达水平。结果经10 μmol·L^-1 Rg1 预处理后,MPP⁺诱导的SHSY5Y 细胞凋亡受到明显的抑制, 虽然线粒体跨膜电位无明显改变, 但细胞内ROS 下降, 而bcl-2 蛋白表达水平增加, bax 蛋白表达水平减少。结论 Rg1 可抑制MPP⁺诱导的SHSY5Y 细胞凋亡,其作用机制可能是通过清除ROS、调节bcl-2、bax 蛋白表达水平起作用。

关键词: 人参皂甙, MPP⁺, SHSY5Y 细胞, 细胞凋亡

Abstract: AIM: To explore the possible anti-apoptotic mechanism of ginsenoside Rg1 on MPP ⁺-induced cellular apoptosis. METHODS: The apoptosis of SHSY5Y induced by MPP⁺was observed by AO-EB staining.Flow cytometry was used to quantitate mitochondrial transmembrane potential (△ψm) and reactive oxygen species (ROS).Western Blot was used to detect the expression of bcl-2 and bax proteins in SHSY5Y cells. RESULTS: MPP⁺-induced apoptosis in SHSY5Y cells was obviously inhibited by pretreatment with 10 μmol·L^-1Rg1.Although there was no difference of mitochondrial transmembrane potential between Rg1 pretreated groups and MPP⁺groups, the level of ROS in Rg1 pretreated groups decreased, the expression of bcl-2 protein increased and expression of bax protein decreased. CONCLUSION: Rg1 protects against MPP⁺-induced apoptosis in SHSY5Y cells and the effect may be attributed to its remove of ROS and its regulation of expression of bcl-2 and bax proteins.

Key words: ginsenoside, MPP⁺, SHSY5Y cells, apoptosis

中图分类号:

285.5

方芳, 陈晓春, 朱元贵. 抗氧化作用可能是人参皂甙Rg1 抗细胞凋亡的机制¹[J]. 中国临床药理学与治疗学, 2002, 7(5): 412-416.

FANG Fang, CHEN Xiao-Chun, ZHU Yuan-Gui. Possible mechanism of mitochondria on anti-apoptotic effect of ginsenoside Rg1 on MPP⁺-induced cellular apoptosis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(5): 412-416.

参考文献

1 Wang CY, Eshleman J, Lutterbaugh J, et al.Spontaneous apoptosis in human colon tumour cell lines and the relation of WT p53 to apoptosis[J].Chin Med J, 1996;109:537-42
2 Cai X, Chen GQ, Jia PM, et al.The mechanisms of arsenic trioxide- induced apoptosis in hematopoietic malignant cells[J]. Natl Med J China(in Chinese), 1999;79:452-5
3 Cassarino DS, Fall CP, Swerdlow RH, et al.Elevated reactive oxygen species and antioxidant enzyme activities in animal and cellular models of Parkinson's disease[J].Biochimica et Biophysica Acta, 1997;1362, 77-86
4 Chen XC, Zhu YG,Wang XZ, et al.Protective effect of ginsenoside Rg1 on dopamine-induced apoptosis in PC12 cells[J].Acta Pharmacol Sin, 2001;22:673-8
5 Li JQ, Zhang XG, Zhang JT.Study on the anti-apoptotic mechanism of ginsenoside Rg1 in cultured cortical neurons[J].Acta Pharm Sin(in Chinese), 1997;32:406-10
6 Cohen G, Fahn S.The oxidant stress hypothesis in Parkinson's disease:evidence supporting it[J].Ann Neurol, 1992;32:804-12
7 Acuna-Castroviejo D.Melatonin is protective against MPTP induced striatal and hippocampal lesions[J].Lif Sci, 1997;60:23-9
8 Sriram K, Pai KS, BoydMR, et al.Evidence for generation of oxidative stress in brain by MPTP:in vitro and in vivo studies in mice[J].Brain Res, 1997;749:44-52
9 Tatton WG, Chalmers-Redman RM.Mitochondriain neurodegenerative apoptosis:an oppoutunity for therapy ? [J].Ann Neurol, 1998;44(3suppl 1):S134-S16
10 Fang F, Chen XC, Zhu YG.MPP+ induced apoptosis in SHSY5Y cells[J].J FUJIAN Medical University, 2001;35: 211-6
11 Chen XC, Zhu YG,Wang XZ, et al.Possible mechanism of the protective effects of ginsenoside Rg1 on apoptosis in PC12 cells [J].Acta Pharm Sin, 2001;36(6):411-4
12 Ziv I, Offen D, Barzilai A, et al.Modulation of control mechanisms of dopamine-induced apoptosis-a future approach to the treatment of Parkinson's disease[J].J Neural Transm Suppl, 1997;49:195
13 Lud J, Harrington B, Ordonez S.bcl-2 overexpression attenuates dopamine-induced apoptosis in an immportalized neural cell line by suppressing the production of reactive oxygen species [J].Synapse, 2000, 35:228

[1]	杨晓佳, 江萌, 吴敏, 张伊黎, 吕兰, 吴嫄芬, 王鑫昱, 刘立权. 藏红花素介导DKK3调控GSK-3β/β-catenin通路对阿尔兹海默症大鼠的认知改善作用[J]. 中国临床药理学与治疗学, 2023, 28(5): 489-497.
[2]	王剑, 孙永东, 周兴玮, 刘雷, 陈龙, 童兴科, 朱佳丽. 黄芩素经由miR-125b-5p/IRF4轴进而促进喉癌细胞死亡并抑制其侵袭的机制研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1209-1218.
[3]	华海燕, 严杰, 秦宇芬. 五味子乙素通过抑制心肌细胞凋亡减轻大鼠心肌缺血再灌注损伤的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(8): 848-856.
[4]	李雪恒, 李龙. 褪黑素与特发性肺纤维化关系的研究进展[J]. 中国临床药理学与治疗学, 2022, 27(6): 715-720.
[5]	李莹, 姚伟, 王萌, 余志宏, 龚园其, 蓝海兵, 齐协飞. 鸢尾素通过NF-κB和JNK信号通路减轻屋尘螨诱导的气道上皮细胞炎症及凋亡的实验研究[J]. 中国临床药理学与治疗学, 2022, 27(10): 1106-1112.
[6]	刘云帆, 杜月梅, 刘晓艺, 高丽萍. 枸杞多糖对顺铂诱导小鼠睾丸支持细胞凋亡的影响[J]. 中国临床药理学与治疗学, 2021, 26(6): 624-630.
[7]	张伟萍, 江建军, 陈晓锋, 许莎莎. 栀子苷通过抑制VPO1/ERK1/2信号通路减轻糖尿病心肌病大鼠的心肌细胞凋亡[J]. 中国临床药理学与治疗学, 2021, 26(2): 129-136.
[8]	沈芸, 张占丰, 王红灵, 赵冀安. 丹酚酸对大鼠骨骼肌缺血再灌注损伤的保护[J]. 中国临床药理学与治疗学, 2020, 25(10): 1111-1118.
[9]	滕晓晶，董学妍，王智毅，余道军，王贤军. 扶正康复合剂联合顺铂抑制胃癌SGC7901细胞荷瘤裸鼠肿瘤增殖作用的实验研究[J]. 中国临床药理学与治疗学, 2019, 24(7): 744-749.
[10]	孔令宇，席錾，杨亚勤，马文婷，吴畏，郝同琴. 地塞米松对小鼠急性胰腺炎胰腺组织中Notch信号及凋亡相关基因表达的影响[J]. 中国临床药理学与治疗学, 2019, 24(2): 134-139.
[11]	齐冰丽，黄平，颜瑞雪，李倩. 柚皮素通过PI3K/AKT/NF-κB通路抑制卵巢癌细胞增殖和侵袭、诱导凋亡作用的研究[J]. 中国临床药理学与治疗学, 2019, 24(11): 1234-1241.
[12]	曹迎亚，陈群，王箴，吴敬医，姜小敢，金孝岠，鲁卫华. 巨噬细胞在脂多糖诱导肠上皮细胞凋亡中的作用[J]. 中国临床药理学与治疗学, 2019, 24(10): 1142-1146.
[13]	刘静，王媚媚，刘录山，贺卫和，庹勤慧，文红艳. 烟酸姜黄素酯对低剪切应力诱导人脐静脉内皮细胞凋亡的影响及相关机制[J]. 中国临床药理学与治疗学, 2018, 23(8): 855-861.
[14]	李宏，陈菁，李丹，余文桃，刘正威，倪峰. 异嗪皮啶对人乳腺癌干细胞凋亡相关基因Bcl-2与Caspase家族表达的影响[J]. 中国临床药理学与治疗学, 2018, 23(8): 886-892.
[15]	张新民，陈鹏，叶盛，夏武杰，李岳春. MG-132对CVB3病毒性心肌炎小鼠心肌细胞凋亡因子的作用研究[J]. 中国临床药理学与治疗学, 2018, 23(5): 498-503.

抗氧化作用可能是人参皂甙Rg1 抗细胞凋亡的机制¹

Possible mechanism of mitochondria on anti-apoptotic effect of ginsenoside Rg1 on MPP⁺-induced cellular apoptosis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价