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中国临床药理学与治疗学 ›› 2002, Vol. 7 ›› Issue (6): 511-514.

• 研究原著 • 上一篇    下一篇

马氏珠母贝全脏器提取物糖胺聚糖抗肿瘤作用的实验研究1

陈方, 吴铁, 艾春媚, 雷晓凌2, 吴红棉2   

  1. 广东医学院药理教研室,湛江 524023;
    2湛江海洋大学水产深加工系,湛江 524025
  • 收稿日期:2002-06-14 修回日期:2002-07-17 出版日期:2002-12-26 发布日期:2020-11-20
  • 通讯作者: 陈方,男,讲师,硕士生,主要从事抗肿瘤药和抗糖尿病药研究。Tel:0759-2388588  E-mail:gdmc.cf@163.com
  • 作者简介:吴铁,男,副教授,硕士生导师,主要从事抗肿瘤药、抗糖尿病药和抗骨质疏松药研究。
  • 基金资助:
    1 本课题为广东省海洋与渔业局科研项目,并获国家知识产权局的发明专利申请初审合格证明书(No00105196.2)

Antitumor effect of glycosaminoglycan from princtade martensi whole viscera in mice1

CHEN Fang, WU Tie, AI Chun-Mei, WU Hong-Mian2, LEI Xiao-Ling2   

  1. Department of Pharmacology,Guangdong Medical College,Zhanjiang 524023;
    2Department of Food Science and Technology,Zhanjiang OceanUniversity,Zhanjiang 524023
  • Received:2002-06-14 Revised:2002-07-17 Online:2002-12-26 Published:2020-11-20

摘要: 目的 观察马氏珠母贝(pinctada martensis)全肉提取物糖胺聚糖精提物(glycosaminoglycan,GAG)或粗提物(coarse glycosaminoglycan,CGAG)对几种肿瘤的作用。方法 用昆明种小鼠,分别观察GAG 和CGAG 对体内几种肿瘤的抑瘤率、生命延长率、与5-FU 或替加氟的协同作用,及其对体外培养HL-60 细胞的杀伤作用。结果 糖胺聚糖粗、精提取物分别对体内S180 肉瘤和艾氏腹水癌(EAC)有显著增敏5-FU 或替加氟的抑瘤作用,抑瘤率分别达61.96%或46.30%;糖胺聚糖精提物对S180 肉瘤生命延长率影响明显,生命延长率为14.16%;糖胺聚糖粗提物(1.0 g·L-1或0.1 g·L-1)对体外培养HL-60 细胞杀伤率达38.67%或25.64%。结论 糖胺聚糖粗、精提物分别对体内S180 肉瘤和EAC 有显著增敏5-FU或替加氟的抑瘤作用,并有一定的提高生命延长率的作用,对体外培养HL-60 细胞还有一定的杀伤作用。

关键词: 马氏珠母贝, 糖胺聚糖, 抗肿瘤

Abstract: AIM: To study the antitumor effect of the crude or the refined extract of glycosaminoglycan(CGAG or GAG)from whole viscera of pinctada martensis.METHODS: The antitumor actions of CGAG or GAG were examined in KM mice inoculated S180 and EAC,including inhibitory rate of tumor growth,the ratio of extending viability,the synergism with 5-FU or Tegafur.The inhibition to the growth of HL-60 cells was observed in vitro.RESULTS: GAG reinforced the antitumor effect of 5-FU or Tegafur in S180 or EAC mice,and the inhibitory rates of tumor growth were 61.96%(S180)and 46.30%(EAC).GAG lengthened life time by 14.16% on S180 mice.The kill rates to HL-60 cells in vitro were 38.67% and 25.64% by using the CGAG(1.0 or 0.1g·L-1).CONCLUSION: GAG can increase the antitumor effects of 5-FU and Tegafur in S180 and EAC mice,lengthen life time in S180 mice,and kill the HL-60 cells in vitro.

Key words: pinctada martensis, glycosaminoglycan, antitumor

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