益智复方对D-半乳糖诱导的小鼠学习记忆障碍的影响1

摘要/Abstract

摘要： 目的: 研究益智复方对痴呆小鼠学习记忆及自由基代谢的影响。方法: 小鼠每日颈背部皮下注射D-半乳糖0.150 g·kg^-1, 连续45 d 可以造成痴呆模型。在注射半乳糖5 d 后, 同时灌胃给予不同剂量的YZC 至造模结束, 再采用Y 型迷宫测验及SOD、MDA 检验试剂盒分别查看其对痴呆小鼠学习记忆及自由基代谢的影响。结果: 益智复方可以显著提高痴呆小鼠Y 型迷宫学习/记忆成绩(所需的训练次数由模型组的31.35 ±8.97/17.12 ±5.57 降低到低中高三个剂量组的19.67 ±8.07/11.27 ±6.65、14.00 ±6.84 8.29 ±5.95 和11.41 ±5.99/6.24 ±4.97);益智复方0.108 g·kg^-1和0.433 g·kg^-1 均可显著提高小鼠脑组织SOD 活性, 从17.69 ±3.14nU·mg^-1pro 升高到26.94 ± 4.46 nU·mg^-1pro 和21.33 ± 3.63 nU·mg^-1pro;而益智复方0.108g·kg^-1、0.217 g·kg^-1 和0.433 g·kg^-1 使小鼠大脑中丙二醛(MDA) 水平由4.08 ±0.88 nmol·mg^-1pro 减少到2.82 ± 0.75 nmol·mg^-1pro、2.10 ± 0.42nmol·mg^-1pro和2.35 ±0.39 nmol·mg^-1pro。结论: 益智复方具有较好的改善学习记忆功能, 这可能与其清除脑内自由基、抑制脂质过氧化的作用有联系。

关键词: 药理学, 益智复方, 学习记忆, 自由基, D-半乳糖

Abstract: AIM: To explore the effects of Compound Yi-Zhi (YZC) on learning and memory capacity and free radical metabolism in D-galactose induced mice dementia model.METHODS: The mice dementia model was induced by a daily D-galactose 0.15 g·kg ^-1 sc for 45 d and after 5 d D-galactose injection, the mice were treated with three doses of YZC once a day for 40 d.In order to find out the influence of YZC on the models learning and memory capability and free radical metabolism system, Ymaze test was introduced for the former and superoxide dismutase (SOD) and malondialdehyde (MDA) tests for the latter.RESULTS: Compared with the D-galactose model group, three doses of YZC administration (0.108, 0.217, 0.433 g·kg ^-1) were all shown to significantly reduced the times of learning/memory in Y-maze test from 31.35 ±8.97/17.12 ±5.57 to 19.67 ±8.07/11.27 ± 6.65, to 14.00 ±6.84/8.29 ±5.95, and to 11.41 ± 5.99 6.24 ±4.97, respectively.For SOD, the administrations of 0.108 and 0.433 g·kg ^-1 YZC could increase the activity of SOD from 17.69 ±3.14 to 26.94 ±4.46, and to 21.33 ±3.63 nU·mg ^-1 pro.While for MDA, all the three doses of YZC could decrease the level of MDA from 4.08 ±0.88 to 2.82 ±0.75, to 2.10 ±0.42, and to 2.35 ±0.39 nmol·mg ^-1 pro in the mice brain tissue, respectively.CONCLUSION: YZC can improve the Dgalactose induced learning and memory dysfunction, and the action may be related to the improvement of antioxidase activity in the model mice brains.

Key words: pharmacology, compound Yi-Zhi, learning, memory, free radical, D-galactose

中图分类号:

R965

徐江平, 吴航宇, 李琳. 益智复方对D-半乳糖诱导的小鼠学习记忆障碍的影响1[J]. 中国临床药理学与治疗学, 2003, 8(1): 31-34.

XU Jiang-Ping, WU Hang-Yu, LI Lin. D-galactose-induced learning and memory deficits in mice¹[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(1): 31-34.

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