关键词: 药理学, 盐酸脂壬仲胺, 重复给药毒性, Beagle 狗, 长期毒性, 安全评价

Abstract: AIM: To evaluate the toxicity of aliphatic nonyl secondary amine hydrochloride administed orally for 180 days in Beagle dogs.METHODS: 24 Beagle dogs were divided into four groups,each consisting of 3 males and 3 females,administered ANSA once daily by oral gavage at doses of 0(control),8.0,2.0,and 0.5mg·kg^-1body weight/day for 6 days/week.The administrationlasted for 180 days.RESULTS: Dogs receiving ANSA at the dose of 8.0 mg·kg^-1showed heterotropia,congestion of conjunctiva,light reflex bluntness,nictitating membrane closed incompletely,and insipidity.Some individuals appeared muscle shivering.All those symptoms emerged 40 minuteslater after administering and holded on 8 hours,disappeared on the next day.The same symptoms in the dose of 2.0 mg·kg^-1group,but those were slighter.Those symptoms were not found in the dose of 0.5 mg·kg^-1grouPand control group.Histopathological examination did not shown any direct injury from the treatment.No abnormity was detected from haematology and serum biochemistry analysis.CONCLUSION: The 0.5 mg·kg^-1of ANSA is a safe dose for oral gavage in Beagle dogs.

Key words: pharmacology, aliphatic nonyl secondary amine hydrochloride, repeated dose toxicity, Beagle dogs, chrinic toxicity, safety evaluation