关键词: 药理学, 银杏内酯, 过氧化氢, c-fos, c-jun, 神经细胞

Abstract: AIM: To observe theProtective effect and mechanism of ginkgolides(Gin)on H₂O₂-induced neurocytes injury.METHODS: H₂O₂was added to the medium of mouse cortical neurons in order to establish H₂O₂-injured model.Neurocytes survival rate was determined by MTT assay,and the expression of c-fos and c-jun genes was analyzed with Western blot.RESULTS: There was reverse concentration-time-relationshiPbetween the treatment of H₂O₂and neurocytes survival rate,while the expression levels of c-fos and c-jun of neurons increased significantly after treatment of H₂O₂.ThePretreatment of Gin for 24 h could relieve the morphologic damage of neurons and enhance the neurocytes survival rate.TheProtective effect of Gin was increased in the range from 0 to37.5 mg ·L-1.Furthermore,Gin could inverse the CJUN expression of neurons induced by H₂O₂,but had no obvious effects on C-FOS.CONCLUSION: GinProtects neurons from H₂O₂-injury via inhibiting over-expression of the c-jun genes.

Key words: pharmacology, ginkgolides, H₂O₂, cfos, c-jun, neuron