高晶体-高胶体渗透压混合液对缺氧/氧再灌损伤的内皮细胞容积和存活率的影响

中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (4): 447-450.

高晶体-高胶体渗透压混合液对缺氧/氧再灌损伤的内皮细胞容积和存活率的影响

黄绍农, 陈建颜, 袁世荧¹

深圳市第二人民医院麻醉科,深圳 518035,广东;
¹华中科技大学同济医学院附属协和医院,武汉 430022,湖北

收稿日期:2003-02-28 修回日期:2003-03-24 出版日期:2003-08-26 发布日期:2020-11-19
通讯作者: 黄绍农,男,硕士,副主任医师,主要研究方向为麻醉治疗学、麻醉与监测。Tel:0755-26176136 　E-mil:huangshaonong@963.net

Effect of hypertonic-hyperoncotic solution on cell volume and survival rate in human umbilical vein endothelial cells suffering from hypoxia/reperfusion

HUANG Shao-Nong, CHEN Jian-Yan, YUAN Shi-Ying¹

Department of Anesthesiology,Shenzhen SecondaryPeople' s Hospital,Shenzhen 518035,Guangdong,China;
¹Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei,China

Received:2003-02-28 Revised:2003-03-24 Online:2003-08-26 Published:2020-11-19

摘要/Abstract

摘要： 目的观察高晶体-高胶体渗透压混合液(HHS)对缺氧/氧再灌损伤的人脐静脉内皮细胞(HUVEC)容积和存活率的影响。方法取HUVEC株ECV-304,分为对照组和实验组。两组细胞先置于缺氧(95 %氮气,5 %CO₂)环境中8 h,然后将实验组细胞置于HHS 15 min,再在常氧状态下(95 %空气,5 %CO₂)培养16 h 。对照组细胞直接置于常氧状态下(95 %空气,5 %CO₂)培养16 h 。两组细胞分别于实验前、实验开始后15 、60 min 和8 、8.25 、16 及24 h 测其细胞容积和存活率。结果对照组细胞在缺氧60 min 后及氧再灌过程中明显肿胀(P<0.05或0.01),细胞存活率进行性降低(P<0.05或0.01)。实验组细胞经HHS 处理后细胞容积较同一时段的对照组细胞容积明显缩小(P<0.01),存活率尽管仍低于实验前(P<0.01),但明显高于同一时段的对照组(P<0.05)。结论 HHS 具有刺激内皮细胞产生延迟性调节性容积减少,保持容积稳定的作用,能在一定程度上降低内皮细胞的死亡率,保护缺氧/氧再灌损伤的内皮细胞。

关键词: 药效学, 高晶体-高胶体渗透压溶液, 人脐静脉内皮细胞, 缺氧/氧再灌, 细胞容积, 存活率

Abstract: AIM: To investigate the effect of hypertonic-hyperoncotic solution(HHS)on cell volume and survival rate in human umbilical vein endothelial cells(HUVEC)suffering from hypoxia/reperfusion.METHODS: HUVEC of ECV-304 were selected and randomly divided into control grouPand experimental group.Cells in two groups were cultured in hypoxia environment(95 %nitrogen,and 5 % carbon dioxide)for 8 h,then in normal environment(95 % air,and 5 % carbon dioxide)for 16 h in control group,and in HHS for 15 min in experimental group.The cell volume and the survival rate were detected before experiment,15 min,60 min,8,8.25,16,and 24 h after experiment in two groups.RESULTS: In control group,cells became significantly inflated and the survival rate decreased in hypoxiaPeriod afterlasting 60 min and hypoxia/reperfusionPeriod(P<0.05 orP<0.01).In experimental group,cells became deflated significantly compared with control grouPat the samePeriod(P<0.01).The survival rate decreased,but significantly higher than that in control grouPat the samePeriod(P<0.05).CONCLUSION: HHS can delay RVD,keePcell volume steady,and decrease cell mortality suffering from hypoxia/reperfusion.

Key words: pharmacodynamics, hypertonic-hyperoncotic solution, human umbilical vein endothelial cells, hypoxia/reperfusion, cell volume, survival rate

中图分类号:

黄绍农, 陈建颜, 袁世荧. 高晶体-高胶体渗透压混合液对缺氧/氧再灌损伤的内皮细胞容积和存活率的影响[J]. 中国临床药理学与治疗学, 2003, 8(4): 447-450.

HUANG Shao-Nong, CHEN Jian-Yan, YUAN Shi-Ying. Effect of hypertonic-hyperoncotic solution on cell volume and survival rate in human umbilical vein endothelial cells suffering from hypoxia/reperfusion[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(4): 447-450.

参考文献

1 Shatos MA,Doherty JA,StumPDC,et al.Oxygen radicals generated during anoxia followed by reoxygenation reduce the synthesis of tissue-typePlasminogen activator andPlasminogen activator inhibitor-1 in human endothelial cell culture[J].J Biol Chem,1990;265:20443-8
2 Kröll W,Pölz W,Colombo T,et al.“Small volume resuscitation”-Erö ffnen sich damit neue M? glichkeiten in der Behandlung des hypovolömischen Schocks[J] ?Wien KlinWochenschr,1994;106:8-12
3 Kletzien RF,Pariza MW,Becker JE,et al.A method using 3-O-Methyl-D-Glucose andPhloretin for the determination of intracellular water space od cells in monolayer culture[J].Anal Biochem,1975;68:537-44
4 Fuijita H,Kuroda Y,Saitch Y.The mechanism of action of the two-layer cold storge method in caninePancresaPreservationprotection ofPancreatic microvascular [J].Kobe J Med Sci,1995;41:47-61
5 WatkinsMT,Haudenschild CC,Al-Badawi H,et al.Immediate responses of endothelial cells to hypoxia and reoxygenation:an in vitro model of cellular dysfunction[J].Am JPhysio,1995;268:749-58
6 Kempski O,Behmanesh S.Endothelial cell swelling and brainPerfusion[J].J Trauma,1997;42:38-40
7 Mazzoni MC,BorgstromP,Warnke KC,et al.Mechanisms and implications of capillary endothelial swelling andluminal narrowing inlow-flow ischemias[J].Int J Microcirc Clin Exp,1995;15:265-70
8 袁世荧,张雪平,曾邦雄.高晶体-高胶体渗透压混合液对脂多糖刺激的人血管内皮细胞ICAM-1mRNA 表达的影响[J].中华麻醉学杂志,2002;22:283-6
9 Corso CO,Okamoto S,Leiderer R,et al.Resuscitation with hypertonic saline dextran reduces endothelial cell swelling and improves hepatic microvascularPerfusion and function after hemorrhagic shock[J].J Surg Res,1998;80:210-20
10 Vollmar MD,Preissler G,Menger MD.Small-volume resuscitation restores hemorrhage-induced microcirculatory disorders in ratPancreas[J].Crit Care Med,1996;24:445-50
11 lang F,Vlkl H,Hussinger D,et al.GeneralPrinciples in cell volume regulation[A].In:Bey enbach K Weds,ed.Cell volume regulation[M].1th ed.Basel:Karger,1990:1-16
12 Kimel berg HK,Goderie S,Khigman S,et al.Swelling-induced release of glutamate,aspartate,and taurinefrom as trocytecultures[J].J Neurosci,1990;10:1583
13 Mc Manus ML,Srange K.Acute volume regulation of brain cells in response to hypertonic challenge[J].Anesthesiology,1993;78:1132
14 lippmann BJ,Yang R,Barnett DW,et al.Pharmacology of volume regulation following hypotonicity-induced cell swelling in clonal N1E115 neuroblas to macells[J].Brain Res,1995;686:29

[1]	武玉洁, 赵程程, 席庆. 蒙特卡洛模拟评价替加环素治疗革兰阴性菌感染给药方案[J]. 中国临床药理学与治疗学, 2023, 28(9): 1027-1033.
[2]	张进华, 刘茂柏, 蔡铭智, 郑英丽, 劳海燕, 向倩, 都丽萍, 朱珠, 董婧, 左笑丛, 李新刚, 尚德为, 陈冰, 叶岩荣, 王玉珠, 高建军, 张健, 陈万生, 谢海棠, 焦正. 模型引导的华法林精准用药：中国专家共识（2022版）[J]. 中国临床药理学与治疗学, 2022, 27(11): 1201-1212.
[3]	徐俪颖王淑华张高松黄继汉黄小民. 和胃止泻胶囊治疗腹泻患者的临床疗效和模型化评价[J]. 中国临床药理学与治疗学, 2022, 27(1): 47-55.
[4]	周晗, 刘晓东. 生理药代动力学模型在创新药物评价中应用及其若干问题的思考[J]. 中国临床药理学与治疗学, 2021, 26(8): 889-913.
[5]	章思亮, 胡琳璘, 邵华, 陈西敬. 性别差异对精神药物作用影响的研究进展[J]. 中国临床药理学与治疗学, 2021, 26(3): 324-331.
[6]	焦正, 李新刚, 尚德为, 董婧, 左笑丛, 陈冰, 刘剑敏, 潘雁, 周田彦, 张菁, 刘东阳, 李禄金, 方翼, 马广立, 丁俊杰, 赵维, 陈锐, 相小强, 王玉珠, 高建军, 谢海棠, 胡蓓, 郑青山. 模型引导的精准用药：中国专家共识（2021版）[J]. 中国临床药理学与治疗学, 2021, 26(11): 1215-1228.
[7]	李文超, 白向荣, 李晓玲, 姜德春. 替加环素在治疗感染性疾病中的群体药代动力学/药效学研究进展[J]. 中国临床药理学与治疗学, 2021, 26(11): 1265-1272.
[8]	向荣凤, 孙凤军, 熊丽蓉, 喻明洁, 戴青, 陈勇川. 左旋泮托拉唑钠肠溶片在健康人体内的药代动力学和药效学研究[J]. 中国临床药理学与治疗学, 2020, 25(8): 903-909.
[9]	彭静, 刘俊, 许慧芳, 李越然, 江佳, 汪盛, 周德喜, 朱艳虹, 杨魁, 栾家杰. 细胞色素P4502C19基因多态性及代谢型与ADP诱导的血小板聚集抑制率及氯吡格雷抵抗的关联性研究[J]. 中国临床药理学与治疗学, 2020, 25(7): 746-751.
[10]	官俏兵，杨毅，郭丽，韩晨阳. 丁苯酞通过内质网应激途径抑制氧化低密度脂蛋白诱导血管内皮细胞凋亡的影响[J]. 中国临床药理学与治疗学, 2019, 24(4): 397-402.
[11]	马广立, 许羚, 陈锐, 陈渊成, 赵维, 刘东阳, 焦正, 李健, 季双敏, 李丽, 李良, 王玉珠, 杨进波, 王亚宁, 孙鹤, 胡蓓, 郑青山, 卢炜. 新药研发中群体药动学/药效学研究的一般考虑[J]. 中国临床药理学与治疗学, 2019, 24(11): 1201-1220.
[12]	高雪梅，张夏丽，陈敏，李洁，罗乐，韩斯嘉，董季，宋振蓉，张轩萍. 白杨素减轻人脐静脉内皮细胞的氧化应激损伤[J]. 中国临床药理学与治疗学, 2018, 23(9): 1022-1026.
[13]	刘静，王媚媚，刘录山，贺卫和，庹勤慧，文红艳. 烟酸姜黄素酯对低剪切应力诱导人脐静脉内皮细胞凋亡的影响及相关机制[J]. 中国临床药理学与治疗学, 2018, 23(8): 855-861.
[14]	张弨. 伏立康唑的药效学研究评价[J]. 中国临床药理学与治疗学, 2018, 23(1): 116-120.
[15]	何秀娟，林燕，蒙玉娇，李萍. 桂皮醛抑制血管内皮细胞与中性粒细胞黏附及其分子机制[J]. 中国临床药理学与治疗学, 2017, 22(1): 43-47.