欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1119-1122.

• 研究原著 • 上一篇    下一篇

霉酚酸酯对2 型糖尿病大鼠肾脏的保护作用

余堂宏, 贾汝汉, 丁国华, 熊燕, 陈健   

  1. 武汉大学人民医院肾内科, 武汉430060, 湖北
  • 收稿日期:2004-07-19 修回日期:2004-08-26 出版日期:2004-10-26 发布日期:2020-11-23
  • 通讯作者: 贾汝汉, 男, 教授, 博士生导师, 主要从事糖尿病肾病等研究。Tel:027-88041919-2149 E-mail:ming-4025 @163. com
  • 作者简介:余堂宏, 男, 硕士研究生, 研究方向为糖尿病肾病。E-mail:yutanghong @126.com

Protective effects of mycophenolate mofetil on kidneys of type 2 diabetic rats

YU Tang-Hong, JIA Ru-Han, DING Guo-Hua, XIONG Yan, CHEN Jian   

  1. Department of Nephrology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei, China
  • Received:2004-07-19 Revised:2004-08-26 Online:2004-10-26 Published:2020-11-23

摘要: 目的: 探讨霉酚酸酯对2 型糖尿病大鼠肾脏的保护作用及其机制。方法: 将实验动物分为正常对照组、糖尿病组及霉酚酸酯治疗组。霉酚酸酯组给予霉酚酸酯(15 mg·kg-1·d-1) 治疗。13 周后检测各组大鼠尿白蛋白排泄率、内生肌酐清除率、血糖、血脂、血胰岛素;HE 及PAS 染色观察肾脏病理改变;免疫组化方法检测肾组织中结缔组织生长因子(CTGF) 及胶原(Col I 、Col Ⅲ) 的表达。结果: 霉酚酸酯可降低尿白蛋白排泄率、内生肌酐清除率、减轻早期肾小球肥大, 且不同程度降低肾组织CTGF 表达及Col I 、Col Ⅲ的沉积。结论: 霉酚酸酯对2 型糖尿病大鼠肾脏有保护作用, 此作用可能与霉酚酸酯降低肾组织中CTGF 的表达, 减少系膜外基质的沉积有关。

关键词: 霉酚酸酯, 糖尿病肾病, 系膜外基质, 结缔组织生长因子

Abstract: AIM: To evaluate the protective effects of mycophenolate mofetil on the kidneys of type 2 diabetic rats and discover their mechanisms.METHODS: Wistar rats were divided into three groups, such as normal control rats, diabetic rats, and diabetic rats in the treatment with mycophenolate mofetil (MMF, 15 mg·kg-1·d-1). Thirteen weeks later, urinary albumin excretory rate (UAE), creatine clearance (Ccr), blood glucose, blood insulin and blood lipid were measured, and kidney pathology was observed. Inmmunohistochemistry was used to analyze the expression of CTGF, ColI and Col Ⅲ.RESULTS: Mycophenolate mofetil decreased UAE, Ccr and reduced glomerular volume. The expression of CTGF and deposition of ECM decreased after diabetic rats received mycophenolate mofetil.CONCLUSION: Mycophenolate mofetil can protect the kidney of diabetic rats. Its mechanism may be related to the decrease of CTGF expression and extracellular matrix deposition in renal tissue.

Key words: mycophenolate mofetil, diabetic nephropathy, extracellular matrix, connective tissue growth factor

中图分类号: