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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1134-1136.

• 研究原著 • 上一篇    下一篇

抗焦虑药物对束缚应激大鼠作用的分子机制探索

黄欢捷, 邵蓓, 郑荣远, 李剑敏1, 王宗敏2, 颜志钦3, 谢丽薇2   

  1. 温州医学院附属一院神经内科,1病理科,2附属二院病理科,3附属三院神经内科, 温州325000, 浙江
  • 收稿日期:2004-08-09 修回日期:2004-09-20 出版日期:2004-10-26 发布日期:2020-11-23
  • 通讯作者: 黄欢捷,男, 医师, 硕士研究生, 主要从事神经内分泌的研究。E-mail:hhuanji e@163. com
  • 作者简介:邵蓓, 女, 教授, 主任医师, 硕士生导师, 研究方向:神经内分泌和神经电生理。郑荣远, 男, 教授, 主任医师, 博士生导师, 研究方向:神经药物流行病学。
  • 基金资助:
    浙江省重点学科基金资助项目(№xxf99005)

Molecular mechanisms of antianxiety drugs on rats with restraint stress

HUANG Huan-Jie, SHAO Bei, ZHENG Rong-Yuan, LI Jian-Min1, WANG Zong-Min2, YAN Zhi-Qin3, XIE Li-Wei2   

  1. Department of Neurology,1Department of Pathology, the First Affiliated Hospital;2Department of Pathology, the Second Affiliated Hospital;3Department of Neurology, the Third Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, Zhejiang, China
  • Received:2004-08-09 Revised:2004-09-20 Online:2004-10-26 Published:2020-11-23

摘要: 目的: 为了探讨抗焦虑药物治疗焦虑症状的分子作用机制。方法: 建立大鼠束缚应激模型, 观察抗焦虑药物帕罗西汀用药组大鼠的旷场行为和下丘脑c-fos 的表达。结果: 与正常对照组比较, 应激大鼠在旷场中的穿行格数和直立次数明显增加, 下丘脑室旁核c-fos 表达显著增加, 而帕罗西汀用药组能减少大鼠在旷场中的活动和下丘脑室旁核c-fos 表达。结论: 帕罗西汀对束缚应激大鼠脑保护作用可能与下调c-fos 基因表达有关。

关键词: 帕罗西汀, 应激, 行为, c-fos, 下丘脑, 室旁核

Abstract: AIM: To investigate the molecular mechanisms of antianxiety drugs on the rats with restraint stress.METHODS: The rat stress model was made by restraint stress. The behaviors of rats were tested in openfield conditions, and the expression of c-fos positive cells was detected by S-P immunohistochemical assay in hypothalamus.RESULTS: The crossing scores, the rearing scores and the expression of c-fos positive cells increased more significantly in the other groups than that in the control group, but decreased in the paroxetine group. The paroxetine inhibited the behaviors and the expression of cfos positive cells in the hypothalamic paraventricular nucleus (PVN)of rats after immobilization stress.CONCLUSION: The effects of paroxetine on the anxiety disorders in rats may be related to the downregulation of the expression of the c-fos in the hypothalamic paraventricular nucleus (PVN).

Key words: paroxetine, stress, behavior, c-fos, hypothalamic, paraventricular nucleus

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