大鼠去卵巢后对不同部位骨骼的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (10): 1175-1178.

大鼠去卵巢后对不同部位骨骼的影响

许碧连, 吴铁, 崔燎, 刘钰瑜, 邹丽宜

广东医学院药理教研室, 湛江524023, 广东

收稿日期:2004-08-04 修回日期:2004-09-14 出版日期:2004-10-26 发布日期:2020-11-23
通讯作者: 许碧连, 女, 硕士研究生, 讲师, 研究方向:骨药理学。Tel:0759-2388588 E-mail:xubilian1@163. net

Effects of ovariectomized on different parts of bones in rats

XU Bi-Lian, WU Tie, CUI Liao, LIU Yu-Yu, ZOU Li-Yi

Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China

Received:2004-08-04 Revised:2004-09-14 Online:2004-10-26 Published:2020-11-23

摘要/Abstract

摘要： 目的: 观察大鼠去卵巢90 d 后不同部位骨骼的变化特点。方法: 4. 5 月龄雌性SD 大鼠20 只, 随机分为假手术组和去卵巢组, 均灌喂5. 6 %乙醇5 ml·kg^-1·d^-1, 持续90 d, 用骨组织形态计量学方法测量胫骨上段和腰椎松质骨及胫骨中段皮质骨的动态和静态参数。结果: 去卵巢大鼠胫骨上段(PTM) 和腰椎(LV) 松质骨的骨量均减少, 其中PTM骨量减少80. 5 %, LV 骨量减少35. 0 %, 胫骨中段(Tx) 皮质骨的骨量未出现丢失, 只是骨内、外膜的骨形成增加。结论: 大鼠去卵巢后呈现高转换型骨质疏松表现, 不同部位的骨骼变化不同。

关键词: 骨质疏松, 骨组织形态计量学, 去卵巢大鼠, 松质骨, 皮质骨

Abstract: AIM: To observe the changes of different parts of bones in rats 90 days after ovariectomized (OVX).METHODS: Twenty 4. 5-month-old virgin female Sprague-Dawley rats were randomly divided into 2 groups: sham group and OVX group. Rats in sham group were sham-operated, while rats in OVX group were bilaterally OVX. Rats in two groups were treated with 5 ml·kg^-1·d^-1 ethanol for 90 days. Parameters of the proximal tibial metaphysis (PTM), tibial shaft (Tx) and the fifth lumbar vertebral body (LVB) were analyzed by bone histomorphometry method.RESULTS: %Tb. Ar and Tb. N were decreased by 80. 5 % and 76. 1 %, Tb. Sp and Oc. N were increased by 468. 0 % and 356. 6 %, and MAR and BFR BV were increased by 43. 9 % and 95. 9 %, respectively, in PTM. The changes in LVB were not remarkable as those in PTM. %Tb. Ar and Tb. Th were decreased by 35. 0 % and 31. 0 %, while Oc. N and BFR BV were increased by 106. 9 % and 126. 8 %, respectively. The cortical bone and marrow areas of tibial shaft did not change in Tx, but bone formation parameters (%P-LPm, %E-LPm ) were increased.CONCLUSION: After OVX for 90 days, high bone turnover osteopenia model is duplicated successfully in rats. Different parts of the bones have different reaction to ovariectomization in rats.

Key words: osteoporosis, bone histomorphometry, ovariectomized rats, cancellous bone, cortical bone

中图分类号:

R965

许碧连, 吴铁, 崔燎, 刘钰瑜, 邹丽宜. 大鼠去卵巢后对不同部位骨骼的影响[J]. 中国临床药理学与治疗学, 2004, 9(10): 1175-1178.

XU Bi-Lian, WU Tie, CUI Liao, LIU Yu-Yu, ZOU Li-Yi. Effects of ovariectomized on different parts of bones in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(10): 1175-1178.

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