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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (11): 1264-1267.

• 研究原著 • 上一篇    下一篇

川芎嗪联用左旋精氨酸对缺血-再灌注损伤兔肝脏能量代谢的影响

王万铁, 金可可, 王卫, 陈锡文1, 徐正柃, 许益笑, 潘雪蓉, 谢克俭2   

  1. 温州医学院病理生理学教研室,1实验动物中心,2分析中心,温州 325027,浙江
  • 收稿日期:2004-08-30 修回日期:2004-11-08 出版日期:2004-11-26 发布日期:2020-11-19
  • 通讯作者: 王万铁,男,教授,硕士研究生导师,主要从事脏器缺血-再灌注损伤的发生机制及其防治的研究Tel: 0577-86689817 E-mail:wzwwt@163.net
  • 基金资助:
    浙江省跨世纪学术和技术带头人基金(No992086);温州市“551人才工程”培养基金(No98113);浙江省卫生厅科研基金(No98A087)

Effects of ligustrazini and L-arginine on hepatocellular energy metabolism in rabbits with reperfusion injury after ischemia

WANG Wan-tie, JIN Ke-Ke, WANG Wei, CHEN Xi-Wen1, XU Zheng-Jie, XU Yai-Xiao, PAN Xue-Rong, XIE Ke-Jian2   

  1. Department of Pathophysiology, 1Experimental Animal Centre, 2Analysis Centre, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China
  • Received:2004-08-30 Revised:2004-11-08 Online:2004-11-26 Published:2020-11-19

摘要: 目的: 探讨川芎嗪(LGT)、左旋精氨酸(L-Arg)联合使用对肝缺血-再灌注损伤(HIRI)时肝细胞能量代谢的影响及其机制。方法: 实验兔40只,建立肝缺血-再灌注模型后随机分4组:模型组,LGT组,L-Arg组和LGT + L-Arg组,每组10只。再灌注45 min时,分别检测肝组织内三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺普(AMP)含量、总腺苷酸量(TAN)、能荷(EC)、丙二醛浓度(MDA)、超氧化物歧化酶活性(SOD)、一氧化氮代谢产物(N02-/N03-)水平、血栓素B2(TXB2)、6-酮基-前列腺素F/6-keto-PGF含量和 TXB2/6-keto-PGF比值。结果: 与模型组比较,LGT组、L-Arg组、LGT + L-Arg组肝组织内 ATP、EC、NO2-/NO3-、6-keto-PGF含量及SOD活性均明显增高(P<0.05和P<0.01),MDA、TXB2含量及TXB2-keto-PGF比值均显著减少(P<0.05和P<0.01)。结论: LGT联用L-Arg可通过降低体内氧自由基水平、提高一氧化氮水平、糾正TXA2与PGI2的平衡,而改善缺血-再灌注损伤肝脏的能量代谢。

关键词: 缺血-再灌注损伤, 肝脏, 能量代谢, 川芎嗓, 左旋精氨酸

Abstract: AIM: To study the effects of ligustrazini (LGT) and L-arginine (L-Arg) on hepatocellular energy metabolism in the rabbits with reperfusion injury after he-patic ischemia and its mechanisms. METHODS: 40 rab-bits were randomly divided into 4 groups (n=10 in each), hepatic ischemia-reperfusion (HIR) group, HIR + LGT group (B), HIR + L-Arg group (C) and HIR + LGT + L-Arg group (D). The contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), ad-enosine monophosphate (AMP),total adenylic acid num-ber (TAN), energy charge (EC), malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide prod-ucts (N02-/N03-), thromboxane B2 (TXB2), and 6-ke-to-prostaglandin F (6-keto-PGF) ratio in the liver tis-sue were measured at 45 minutes after reperfusion. RESULTS: The contents of ATP, NO, 6-keto-PGF and SOD activity of the liver tissue in B, C, and D groups were higher than those in HIR group (P < 0.05 or P < 0.01), the contents of MDA, TXB2 and TXB2/6-keto-PGF ratio of the liver tissue in B,C, and D groups were lower than those in HIR group (P<0.05 or P<0.01). CONCLUSION: The combination usage of LGT and L-Arg can improve the hepatocellular energy metabolism in the reperfusion injury after liver ischemia by decreasing oxygen free radical level, raising NO level and correcting TKA2/PGI2 imbalance.

Key words: hepatic ischemia-reperfusion injury, energy metabolism, ligustrazini, L-Arginine

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