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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 230-233.

• 研究原著 • 上一篇    下一篇

川芎嗪对小鼠急性肾缺血再灌注损伤的保护作用研究

霍展样, 牛桂萍1, 刘晓丽   

  1. 新乡医学院机能学实验室, 新乡 453003, 河南;
    1焦煤集团中央医院, 焦作 454150, 河南
  • 收稿日期:2003-06-04 修回日期:2003-08-29 出版日期:2004-02-26 发布日期:2020-11-16
  • 通讯作者: 霍展样,女, 实验师, 主要从事生理学实验教学与科研和机能学实验教学工作。Tel:0373-3029405  E-mail: huo_zhan_yang@sina.com

Protective effects of ligustrazine on renal damage of acute ischemia-reperfusion in mice

HUO Zhan-Yang, NIU Gui-Ping1, LIU Xiao-Li   

  1. Functionnal Laborary of Xinxiang Medical College, Xinxiang 453003, Henan, China;
    1the Lenter Hospital of Jiaomei Conglomerate, Jiaozuo 454150, Henan, China
  • Received:2003-06-04 Revised:2003-08-29 Online:2004-02-26 Published:2020-11-16

摘要: 目的: 探讨川芎嗪(LT)对小鼠急性肾缺血再灌注损伤的保护作用。方法: 夹闭右肾肾蒂阻断血流30 min, 然后放开动脉夹再灌注0.25 、1 、24 h, 造成小鼠急性缺血再灌注模型, 于手术前分别腹腔注射川芎嗪100 mg·kg-1 和等量生理盐水。化学法观察小鼠血清肌酐(Scr)、尿素氮(Bun)、丙二醛(MDA)含量和超氧化物歧化酶(SOD), 肾组织MDA 含量和SOD, 并观察肾组织WBC 滞留数、肾小管计分等病理变化。结果: 与模型组比较, 川芎嗪使肾缺血再灌注1 、24 h 小鼠肾组织MDA 含量、肾小管计分明显降低, 血、肾中SOD 活性增强, 使肾缺血再灌注24 h血中Scr 、Bun 、MDA 含量、肾中WBC 滞留数明显降低。结论: 川芎嗪对急性肾缺血再灌注损伤有保护作用, 其机制可能与抗自由基损伤、抑制WBC 粘附有关。

关键词: 川芎嗪, 肾缺血再灌注, 抗氧化, WBC 粘附

Abstract: AIM: To investigate the effects of ligustrazine (LT)on renal damage of acute ischemia-reperfusion in mice.METHODS: The model of renal ischemiareperfusion in mice was established by clamping the right renal pedicel in situ for 30min before reperfusion of blood 15 min, 60 min, and 24 h.Treated the mice with LT100 mg·kg-1 ip (ligustrazine group)and saline ip (control group)30 min before operation.The levels of serum creatinine (Scr), blood urea nitrogen (Bun), content of malondialdehyde (MDA)and the activity of superoxide dismutase (SOD)in serum and renal tissue, numbers of WBC adhesion in renal tissue and scores of tubules were detected.RESULTS: LT lowered significantly MDA content and scores of tubules, the number of WBC adhesion, the levels of Scr, BUN, and increased the activity of SOD in renal tissues and serum.CONCLUSION: Ligustrazine may protect the renal damage of acute ischemiareperfusion, and the mechanism is to antagonize the free radical injury and to inhibit WBC adhesion.

Key words: ligustrazine, renal ischemia-reperfusion, anti-oxidation, WBC adhesion

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