土贝母苷甲诱导HeLa 细胞周期阻滞和凋亡

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (3): 261-269.

土贝母苷甲诱导HeLa 细胞周期阻滞和凋亡

马润娣, 于立坚, 苏伟明, 邵海艳, 廖铭能, 何冬梅, 黄来珍

湛江海洋大学, 广东省海洋药物重点实验室, 湛江524025, 广东

收稿日期:2003-07-03 接受日期:2003-08-28 出版日期:2004-03-26 发布日期:2020-11-21

Induction of cell cycle arrest and apoptosis by tubeimoside I isolated from Bolbostemma paniculatum in HeLa cells

MA Run-Di, YU Li-Jian, SU Wei-Ming, SHAO Hai-Yan, LIAO Ming-Neng, HE Dong-Mei, HUANG Lai-Zhen

Guangdong Provincial Key Laboratory of Marine Materia Medica,Zhanjiang Ocean University, Zhanjiang 524025, Guangdong, China

Received:2003-07-03 Accepted:2003-08-28 Online:2004-03-26 Published:2020-11-21
Contact: YU Li-Ji an, male, senior researcher, tutor of master,engaged in the separation of natural anti tumor drug, the molecular biologi cal and biochemical mechanisms of the tumor occurrenc, and the neurotoxi city of excitatory amino acids.Tel:0759-2382424 　E-mail:ywyj9578@sohu.com
Supported by:
Project supported by the Natural Science Foundation of Guangdong Province(№011809)

摘要/Abstract

摘要： 目的:研究土贝母苷甲(下称苷甲)抗肿瘤作用的机制, 探索它对细胞周期和细胞凋亡的影响。方法:采用MTT 法检测苷甲对细胞生长的抑制作用;采用流式细胞术, 光学、荧光和电子显微镜, 以及DNA 琼脂糖凝胶电泳检查和分析苷甲对细胞周期和细胞凋亡的影响;采用蛋白免疫印迹法检测凋亡相关基因bcl-2 和bax 表达的变化。结果:苷甲对HeLa 细胞的生长有强抑制作用, 其24、48 和72 h 的IC₅₀ 值分别为35.7, 23.6, 17.4 μmol·L^-1 。在苷甲的作用下, 细胞周期阻滞在G₂/M 期, 细胞在形态学和生物化学上都出现了细胞凋亡的典型证据。蛋白免疫印迹结果揭示bcl-2 下调, bax 过表达。结论:苷甲诱导的细胞周期阻滞和凋亡在苷甲的抗肿瘤效果中可能起着重要作用, 而苷甲诱导的细胞凋亡与bcl-2 的下调及bax 的过表达密切相关。

关键词: 土贝母, 土贝母皂苷, 土贝母苷甲, 细胞周期, 细胞凋亡, bcl-2, bax, HeLa 细胞

Abstract: AIM: To study the antitumor mechanism of tubeimoside I (TBMS1), and to investigate its effects on cell division cycle and cell death in HeLa cells. METHODS: Cell growth inhibition of TBMS1 was measured by MTT assay;the induction of cell cycle arrest and apoptosis by TBMS1 was determined by flow cytometry, light, fluorescence and electron microscopies, and gel electrophoresis of fragmented DNA.Western blotting was performed for detecting the apoptosis-related genes.RESULTS: TBMS1 displayed strong growth inhibitory effect against HeLa cells with estimated IC₅₀ values of 35.7, 23.6, and 17.4 μmol·L ^-1.HeLa cells underwent G₂/M arrest with exposure to TBMS1, and had typical morphologic and biochemical evidence of apoptosis.Western blot analysis of TBMS1-treated cells revealed downregulation of bcl-2, and overexpression of bax.CONCLUSION: TBMS1-induced cell cycle arrest and apoptosis may play an important role in antitumor effect of TBMS1, and TBMS1-induced apoptosis is closely related to downregulation of bcl-2 and overexpression of bax.

Key words: Bolbostemma paniculatum, tubeimoside I, cell cycle arrest, apoptosis, bcl-2, bax, HeLa cells

中图分类号:

R965
R979.1

马润娣, 于立坚, 苏伟明, 邵海艳, 廖铭能, 何冬梅, 黄来珍. 土贝母苷甲诱导HeLa 细胞周期阻滞和凋亡[J]. 中国临床药理学与治疗学, 2004, 9(3): 261-269.

MA Run-Di, YU Li-Jian, SU Wei-Ming, SHAO Hai-Yan, LIAO Ming-Neng, HE Dong-Mei, HUANG Lai-Zhen. Induction of cell cycle arrest and apoptosis by tubeimoside I isolated from Bolbostemma paniculatum in HeLa cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(3): 261-269.

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