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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (3): 305-307.

• 研究原著 • 上一篇    下一篇

醋柳黄酮对血管平滑肌细胞胞内游离钙浓度的影响

李家富, 章茂顺1, 王家良1, 符宗胤1   

  1. 泸州医学院附属医院心内科, 泸州646000, 四川;
    1华西医科大学附一院心内科, 成都610041, 四川
  • 收稿日期:2003-11-04 修回日期:2004-02-03 出版日期:2004-03-26 发布日期:2020-11-21
  • 通讯作者: 李家富, 男, 硕士, 副教授, 主要从事心血管疾病的基础与临床研究。Tel:13882796628  E-mail:LJF-198@163.com

Effects of flavonoids of Hippophae rhamnoides L on intracellular free calcium concentration in cultured rabbit aortic smooth muscle cells

LI Jia-Fu, ZHANG Mao-Shun1, WANG Jia-Liang1, FU Zhong-Yin1   

  1. Department of Cardiology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan, China;
    1Department of Cardiology, First Affiliated Hospital, WCUMS, Chengdu 610041, Sichuan, China
  • Received:2003-11-04 Revised:2004-02-03 Online:2004-03-26 Published:2020-11-21

摘要: 目的:探讨醋柳总黄酮(TFH) 对血管平滑肌细胞胞内游离钙浓度([Ca2+]i) 的影响。方法:采用新一代钙荧光探针Fluo-3/AM 检测在高钾、去甲肾上腺素(NE)、血管紧张素II(Ang Ⅱ) 刺激下单层兔主动脉平滑肌细胞内游离钙水平的改变, 并与传统的钙拮抗剂Verapamil (Ver) 进行对照研究。结果:TFH(100 mg·L-1) 对静息状态的血管平滑肌细胞[Ca2+]i 无明显影响;TFH(60 ~ 100 mg·L-1) 呈剂量依赖性抑制高K+ 去极化引起的[Ca2+]i 升高, 与Ver 作用相似, 但弱于Ver;TFH(80、100 mg·L-1) 对NE、Ang Ⅱ通过受体介导引起[Ca2+]i 升高均具有明显的抑制作用;在无细胞外Ca2+存在下, TFH(80、100 mg·L-1) 对NE 引起的[Ca2+]i 升高也具有一定程度的抑制效应。结论:醋柳总黄酮通过对电压依赖性钙通道和受体操纵型钙通道双重抑制降低血管平滑肌细胞内游离钙水平, 这可能是醋柳黄酮产生舒血管降压作用机制之一。

关键词: 醋柳黄酮, 血管平滑肌细胞, 胞内游离钙浓度, 钙拮抗剂,

Abstract: AIM: To explore the effects of total of flavonoid Hippophae rhamnoides L (TFH) on intracellular free calcium ([Ca2+]i) in vascular smooth muscle cells (VSMC).METHODS: The cultured rabbit aortic smooth muscle cells were expose to high K+, norepinephrine (NE) and angiotensin II(Ang Ⅱ), which were compared with those of verapamil(Ver), and then the changes of [Ca2+]i in VSMC were determined by using the new generation of fluorescent Ca2+-indicater fluo-3/AM.RESULTS: TFH (100 mg·L -1) had no significant effect on theresting[Ca2+]i.However, and TFH(60 -100 mg·L -1) inhibited the elevation of [Ca2+]i induced by high potassium-depolarization in a concentration-dependent manner. These effects were similar to but weaker than those of Ver.In addition, TFH (80, 100 mg·L -1) inhibited the elevation of [Ca2+]i induced by NE or Ang II in the presence of extracellular Ca2+.In the absence of extracellular Ca2+, TFH (80, 100 mg·L -1) also had some blocking effects on the NE-induced [Ca2+]i increase.CONCLUSION: TFH may decrease the [Ca2+]i of VSMCs by blocking both voltage-dependent calcium channels and receptor-operated calcium channels, which may be one of TFH vasodilative and hypotensive mechanisms.

Key words: total flavonoids of Hippophae rhamnodies L, vascular smooth muscle cell, intracellular free calcium, calcium antagonist, rabbit

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