银杏叶提取物对豚鼠心室肌细胞动作电位及L 型钙离子通道的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (3): 345-349.

银杏叶提取物对豚鼠心室肌细胞动作电位及L 型钙离子通道的影响

张绪国, 王家宁¹, 李建军², 刘忠武³, 黄从新²

郧阳医学院附属太和医院心内科, ¹附属人民医院心内科, 3中心实验室, 十堰442000, 湖北;
²武汉大学人民医院心内科, 武昌430000, 湖北

收稿日期:2003-10-16 修回日期:2003-11-28 出版日期:2004-03-26 发布日期:2020-11-21
通讯作者: 张绪国, 男, 硕士研究生, 副主任医师, 郧阳医学院附属太和医院心内科, 研究方向:心脏电生理。Tel:0719-8801430 　E-mail:sysyxl@163.com

Effects of Ginkgo biloba extract on action potential and L-type calcium channel in guinea pig's ventricular myocytes

ZHANG Xu-Guo, WANG Jia-Ning¹, LI Jian-Jun², LIU Zhong-Wu³, HUANG Chong-Xing²

Department of Cardiology, the Affiliated Taihe Hospital, Yunyang Medical College, Shiyan 442000, Hubei, China;
¹Department of Cardiology, the Affiliated People's Hospital;³the Key Labratry;²Department of Cardiology, the People's Hospital, Wuhan Uaiversity, Wuhan 430000, Hubai, China

Received:2003-10-16 Revised:2003-11-28 Online:2004-03-26 Published:2020-11-21

摘要/Abstract

摘要： 目的:观察银杏叶提取物(EGB)对豚鼠心室肌细胞动作电位和L-型钙通道的影响。方法:采用全细胞膜片钳技术的电流钳记录动作电位和电压钳记录L-型钙离子通道电流(I_Ca-L)。结果:银杏叶提取物(原液含银杏黄酮甙0.804 g·L^-1, 银杏内酯0.06 g·L^-1)明显缩短心室肌细胞动作电位时程(APD), 1 ∶800 稀释液致APD₉₀ 缩短13 %(P <0.05), 1 ∶200 稀释液致APD₉₀缩短23 %(P <0.05);银杏叶提取物对心肌细胞L-型钙离子通道的开放有抑制作用, 在1 ∶800 时I_Ca-L 峰值降低15 %(P <0.05), 1 ∶200 时I_Ca-L 峰值降低42 %(P <0.05), 均呈浓度依赖性, 随着药物浓度的增加, I-V 关系曲线逐渐上移, 但出现电流峰值的电压不变。结论:银杏叶提取物能明显缩短APD, 抑制心肌细胞钙离子通道的开放, 具有明显的浓度依赖关系, 可能是其抗心律失常的分子基础。

关键词: 银杏叶提取物, 膜片钳全细胞记录, 心室肌细胞, 动作电位, L-型钙电流

Abstract: AIM: To study the effects of Ginkgo biloba extract (EGB)on action potential (AP)and L-type calcium channel in isolated guinea pig's ventricular myocytes. METHODS: AP was recorded by current-clamp mode and I_Ca-L was recorded by voltage-clamp mode using whole patch clamp technique.RESULTS: Action potential duration of repolarization 90 %(APD₉₀)was shortened by 13 %, 23 % at the dilution of EGB 1 ∶800, and 1 ∶ 200, respectively (P <0.05).L-type calcium current (I_Ca-L)was inhibited by EGB significantly in a concentration-dependent manner.Peak I_Ca-L was reduced by 15 %at the dilution of EGB 1 ∶800 and 42 %at 1 ∶200, respectively(P <0.05).I-V relationship and the voltage of peak current did not change significantly.CONCLUSION: EGB can shorten APD and inhibit I_Ca-L in a concentration-dependent manner, which may be one of critical basis on antiarrhythmia.

Key words: Ginkgo biloba extract, patch clamp, myocardium, action potential, L-type calcium current

中图分类号:

张绪国, 王家宁, 李建军, 刘忠武, 黄从新. 银杏叶提取物对豚鼠心室肌细胞动作电位及L 型钙离子通道的影响[J]. 中国临床药理学与治疗学, 2004, 9(3): 345-349.

ZHANG Xu-Guo, WANG Jia-Ning¹, LI Jian-Jun², LIU Zhong-Wu³, HUANG Chong-Xing². Effects of Ginkgo biloba extract on action potential and L-type calcium channel in guinea pig's ventricular myocytes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(3): 345-349.

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