白花前胡提取物对腹主动脉缩窄大鼠心室重构及Bcl-2、Bax 蛋白表达的影响

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (4): 394-398.

白花前胡提取物对腹主动脉缩窄大鼠心室重构及Bcl-2、Bax 蛋白表达的影响

涂欣, 王晋明, 涂乾¹, 涂晋文²

武汉大学人民医院心血管内科, 武汉430060, 湖北;
¹江汉大学医学与生命科学院医学系, 武汉430000, 湖北;
²湖北中医学院附属医院内科, 武汉430061, 湖北

收稿日期:2003-12-15 修回日期:2004-02-22 出版日期:2004-04-26 发布日期:2020-11-20
通讯作者: 涂欣, 男, 博士研究生, 研究方向为心血管疾病。Tel:027-62001880 E-mail:partner@publi c.wh.hb.cn
作者简介:王晋明, 男, 博士生导师, 研究方向:心血管疾病。涂晋文, 男, 博士生导师, 研究方向:中医内科心脑血管疾病。涂乾, 女, 博士研究生。
基金资助:
武汉市科技攻关计划基金项目(№302200205)

Effects of Peucedanum praeruptorum Dunn Extract on left ventricular remodeling and Bcl-2, Bax protein expression in aorta coarctation rats

TU Xin, WANG Jin-Ming, TU Qian¹, TU Jin-Wen²

Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China;
¹Medical and Life Science School of Jianghan University, Wuhan 430000, Hubei, China;
²Department of Internal Medicine of Attached Hospital of Hubei College of Chinese Medicine, Wuhan 430061, Hubei, China

Received:2003-12-15 Revised:2004-02-22 Online:2004-04-26 Published:2020-11-20

摘要/Abstract

摘要： 目的:研究白花前胡提取物(PdE) 对腹主动脉缩窄术后大鼠心室重构及心肌组织凋亡相关蛋白Bcl-2、Bax 表达的影响。方法:部分缩窄大鼠腹主动脉术制作心肌肥厚模型。称重法检测左室心肌重量, 计算心系数;观察细胞组织结构改变, 测量左心室内径;免疫组织化学法检测凋亡相关基因及Bcl-2、Bax 蛋白表达。结果:腹主动脉缩窄术后, 大鼠左室重量及心系数明显增加;400 %PdE 可明显减轻大鼠腹主动脉术所致左室重量增加(用药组0.49 ±0.07 g, 模型组0.57 ±0.16 g, P <0.05) 及心系数的升高(用药组2.44 %, 模型组2.82 %, P <0.05);200 % PdE 亦可明显降低大鼠腹主动脉术后心系数的升高(用药组2.51 %, 模型组2.82 %, P <0.05);腹主动脉缩窄术后大鼠心肌细胞明显肥大,胞核呈浓染、增大, 细胞间距增大, 组织横断面心肌纤维增多;400 % PdE 及200 % PdE 可有效改善因腹主动脉缩窄所致心肌组织结构变化;腹主动脉缩窄术后, 大鼠左室腔内径明显缩小, 而400 % PdE 及200 % PdE 可显著改善大鼠腹主动脉术后左室腔内径缩小(P <0.05);模型组Bcl-2 与Bax 蛋白表达率与伪手术组、各浓度PdE 组比较均有显著性差异(P<0.05), 但伪手术组, 400 %及200 % PdE 组Bcl-2/Bax 表达比例均较模型组有明显增加(P <0.05) 。结论:PdE 能有效改善因腹主动脉缩窄所致的心室重构过程中的左心室壁肥厚和心系数增加, 调节心肌细胞凋亡相关蛋白Bcl-2 与Bax 的表达。

关键词: 白花前胡提取物, 腹主动脉缩窄, 心室重构, 心肌肥厚, 凋亡相关蛋白

Abstract: AIM: To investigate the effects of Peucedanum praeruptorum Dunn Extract on left ventricular remodeling and apoptosis-related protein(Bcl-2, Bax) expression in aorta coarctation rats.METHODS: Partly aorta coarctation was practiced in Wistar rats.The weight of the left ventricular and the cardiac index were measured;the diameter of the left ventricular cavity was measured by means of HE dyeing;the expression of Bcl-2 and Bax protein in myocardium was observed by immunohistochemical method.RESULTS: After partly aorta coarctation, the weight of the left ventricular and the cardiac index of rats were increased significantly.Compared with the model group, 400 % PdE decreased the left ventricular and the cardiac index effectively and 200 % PdE decreased the increasing cardiac index in rats which after aorta coarctation.In the model group, the myocardial cells were much bigger and there were much more parallel connections and series connections of sarcomere;compared with those in the model group, the myocardial cells were nearly normal or just had a slight degree of hypertrophy and hyperplasia in both 400 % PdE and 200 % PdE groups.Compared with the model group, the 400 % PdE and 200 %PdE groups had bigger diameter of left ventricular cavity, the difference was significant (P <0.05); compared with the model group, 400 % and 200 % PdE significantly increased the proportion of Bcl-2 and Bax protein expression in the myocardial cells of rats after the aorta coarctation.CONCLUSION: Peucedanum praeruptorum Dunn Extract can affect the remodeling process of ventricular muscles and the apoptosis-related proteins in different ways and had positive importance in treatment of ventricular remodeling.Therefore, it deserves further researching and developing.

Key words: Peucedanum praeruptorum Dunn Extract, aorta coarctation, ventricular remodeling, myocardium hypertrophy, apoptosis-related protein

中图分类号:

R965.1

涂欣, 王晋明, 涂乾, 涂晋文. 白花前胡提取物对腹主动脉缩窄大鼠心室重构及Bcl-2、Bax 蛋白表达的影响[J]. 中国临床药理学与治疗学, 2004, 9(4): 394-398.

TU Xin, WANG Jin-Ming, TU Qian, TU Jin-Wen. Effects of Peucedanum praeruptorum Dunn Extract on left ventricular remodeling and Bcl-2, Bax protein expression in aorta coarctation rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(4): 394-398.

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