17 β-雌二醇增加卵巢切除大鼠心肌内皮型一氧化氮合酶的表达和一氧化氮生成

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (4): 411-416.

17 β-雌二醇增加卵巢切除大鼠心肌内皮型一氧化氮合酶的表达和一氧化氮生成

汤勇波, 王乾蕾, 朱炳阳, 黄红林, 廖端芳

南华大学药物药理研究所, 衡阳421001, 湖南

收稿日期:2003-12-09 修回日期:2004-02-17 出版日期:2004-04-26 发布日期:2020-11-20

Study of 17 β-Estradiol supplementation on increasing eNOS expression and NO production in ovariectomized rat heart

TANG Yong-Bo, WANG Qian-Lei, ZHU Bing-Yang, HUANG Hong-Lin, LIAO Duan-Fang

Institute of Pharmacy and Pharmacology, Nanhua University, Hengyang 421001, Hunan, China

Received:2003-12-09 Revised:2004-02-17 Online:2004-04-26 Published:2020-11-20
Contact: HUANG Hong-Lin, professor, tutor of master, engaged in protective efficacy of cardiovascular system of estrogen.Tel:0734-8281408 Fax:0734-8281239 E-mail:huanghonglinhui@yahoo.com.cn
Supported by:
This work was kindly supported by the Hunan Provinci al Bureau of Health Traditional Drug Foundation(№202072)

摘要/Abstract

摘要： 目的:观察17β-雌二醇替代治疗对卵巢切除大鼠心肌组织中一氧化氮(NO) 和内皮源性一氧化氮合酶(eNOS) 的影响。方法:成年雌性SD 大鼠经双侧卵巢切除术, 假手术组作为对照, 术后3 周随机分为假手术组, 模型对照组, 17β-雌二醇(0.1 mg·kg^-1·d^-1) 和溶媒(芝麻油) 皮下注射治疗组, 处理6 周, 记录大鼠的血压、心率、子宫重量;检测血中雌二醇的含量;亚硝酸还原酶法检测心肌匀浆中NO 含量;Western blot 分析心肌中eNOS 及eNOS 调节蛋白小凹蛋白-1(caveolin-1) 的蛋白表达情况。结果:17β-雌二醇治疗组的心率(314 ±16 次/分) 较其他各组低, 与溶媒对照组比较, 雌二醇替代治疗组心肌匀浆中NO 的含量增加一倍, eNOS 蛋白表达明显增加(P <0.01), 而作为eNOS 的内源性抑制物caveolin-1 的表达却明显减少(P <0.05) 。结论:雌激素替代治疗直接增加卵巢切除大鼠心肌eNOS 的表达量以及NO 的产生, 减少抑制eNOS 活性的小凹蛋白1 表达。

关键词: 17β-雌二醇, 一氧化氮, 内皮型一氧化氮合酶, 小凹蛋白

Abstract: AIM: To investigate whether 17β-estradiol influences the production of nitric oxide (NO) and expression of eNOS in ovariectomized (OVX) rats heart. METHODS: Female mature Sprague–Dawley rats were subjected to a bilateral ovariectomy, and sham operation as control.OVX rats were randomly assigned to the following treatments:17β-estradiol (100 μg·kg^-1 in 100 μl sesame oil sc daily), 17β-estradiol vehicle (100 μl sesame oil sc daily) and OVX control.The treatment lasted 6 weeks.Blood pressure, heart rate, plasma estradiol, uterine weights and nitrite production in the myocardium were studied.Western blot analysis was used to assay the protein expression of eNOS and caveolin-1.RESULTS: The heat rates in 17β-estradiol treatment group (314 ±16 beats·min^-1) were lower than those in other groups (P <0.05).Compared with vehicle group, nitrite production increased one fold in the hearts of E2 treatment group, the abundance of eNOS protein showed a significant increase (P <0.01).Concurrently caveolin-1 protein, an endogenous eNOS inhibitor, protein expression abundance was significantly decreased (P <0.05) in the E2 supplement group.CONCLUSION: Estrogen supplementation directly increases eNOS functional activity and NO production in rat heart, this effect may be related to estrogen-induced deceasing of heart rates.

Key words: 17β-estradiol, nitric oxide, endothelial nitric oxide synthase, caveolin

中图分类号:

R965.1
R963

汤勇波, 王乾蕾, 朱炳阳, 黄红林, 廖端芳. 17 β-雌二醇增加卵巢切除大鼠心肌内皮型一氧化氮合酶的表达和一氧化氮生成[J]. 中国临床药理学与治疗学, 2004, 9(4): 411-416.

TANG Yong-Bo, WANG Qian-Lei, ZHU Bing-Yang, HUANG Hong-Lin, LIAO Duan-Fang. Study of 17 β-Estradiol supplementation on increasing eNOS expression and NO production in ovariectomized rat heart[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(4): 411-416.

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