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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (4): 426-429.

• 研究原著 • 上一篇    下一篇

IL-10 与可溶性鸡Ⅱ型胶原对实验性关节炎的联合治疗作用

卢延旭, 陈敏珠1, 路景涛1   

  1. 中国刑事警察学院法医系法医毒理研究室, 沈阳110035, 辽宁;
    1安徽医科大学临床药理学研究所, 合肥230032, 安徽
  • 收稿日期:2003-12-18 修回日期:2004-02-25 出版日期:2004-04-26 发布日期:2020-11-20
  • 通讯作者: 卢延旭, 男, 医学博士, 教授, 博士生导师, 研究方向:免疫药理学与毒理学。Tel:024-86982962 86982276 E-mail:yanxulu4689@yahoo.com.cn
  • 作者简介:陈敏珠, 女, 教授, 博士生导师, 研究方向:免疫药理学。Tel:0551-2672390 E-mail:chenmz@yahoo.com.cn

Combined therapeutic effects of recombinant human IL-10 with soluble chicken type Ⅱ collagen on experimental arthritis

LU Yan-Xu, CHEN Min-Zhu1, LU Jing-Tao1   

  1. Department of Forensic Toxicology, China Criminal Police College, Shenyang 110035, Liaoning, China;
    1Institute of Clinical Pharmacology, Anhui Medical University, Hefei 230032, Anhui, China
  • Received:2003-12-18 Revised:2004-02-25 Online:2004-04-26 Published:2020-11-20

摘要: 目的:考察重组人白细胞介素-10(rhIL-10) 对可溶性鸡Ⅱ型胶原(SCC Ⅱ) 治疗大鼠佐剂性关节炎(AA) 疗效的影响。方法:通过测量足肿胀度、体重、胸腺与脾脏系数, 观察不同部位淋巴细胞ConA 增殖反应并检测腹腔巨噬细胞(PMΦ) 与滑膜组织细胞(SMCs) 产生IL-1 的水平。结果:与SCC Ⅱ(0.03 mg·kg-1·d-1, ig) 或IL-10(1, 2 μg·d-1, sc) 单独治疗比较, 二者联合治疗大鼠AA, 明显改善上述观察指标, 同时使SCC Ⅱ的口服耐受诱导期缩短, 增强了治疗效果。结论:IL-10 能提高SCC Ⅱ治疗大鼠AA 的治疗效果。

关键词: 可溶性鸡Ⅱ型胶原, rhIL-10, 联合治疗, 佐剂性关节炎, 淋巴细胞增殖反应, 滑膜组织细胞, 腹腔巨嗜细胞, 白细胞介素-1

Abstract: AIM: To investigate the combined therapeutic effects of recombinant human interleukin-10 (rhIL-10) with soluble chicken collagen type Ⅱ (SCC Ⅱ) on rat adjuvant arthritis(AA).METHODS: The paw swelling, gain in body weight, thymus-and spleen-indices, the lymphocyte proliferative responses induced by ConA and the levels of IL-1 in the peritoneal macrophages (PMΦ) and synovial membrane tissue cells (SMCs) in rats were examined. RESULTS: Compared with SCC Ⅱ (0.03 mg·kg-1 ·d-1, ig) or rhIL-10 (1, 2 μg·d-1, sc) treatment alone, combined treatment of rhIL-10 (1,2 μg·d-1, sc) with SCC Ⅱ (0.03 mg·kg-1·d-1, ig) significantly improved the above indices, shortened the induced phase of rat oral tolerance (OT) to SCC Ⅱ, and strengthened the treatment effect.CONCLUSION: rhIL-10 can improve the therapeutic effect of SCC Ⅱon rat AA.

Key words: soluble chiken collagen type II, recombinant human interleukin-10, combined treatment, adjuvant arthritis, lymphocyte proliferative responses, synovial membrane tissue cells, peritoneal macrophage, interleukin-1

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