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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (4): 434-437.

• 研究原著 • 上一篇    下一篇

小牛血去蛋白提取物注射液对培养的心肌细胞缺氧再给氧损伤的保护作用

杨育红, 娄凤霞, 王洪新   

  1. 锦州医学院药理学教研室, 锦州121001, 辽宁
  • 收稿日期:2003-12-02 修回日期:2004-02-06 出版日期:2004-04-26 发布日期:2020-11-20
  • 通讯作者: 王洪新, 男, 医学博士, 教授, 硕士研究生导师, 研究方向:心血管药理学。Tel:0416-4580209 E-mail:jyhxwang@hotmail.com
  • 作者简介:杨育红, 女, 医学硕士, 副教授, 研究方向为心血管药理学。Tel:0416-4673111 E-mail:yangyh88@21cn.com

Protective effects of deproteinized extract of calf blood on hypoxia-reoxygenation-induced injury in cultured myocardial cells

YANG Yu-Hong, LOU Feng-Xia, WANG Hong-Xin   

  1. Department of Pharmacology, Jinzhou Medical College, J inzhou 121001, Liaoning, China
  • Received:2003-12-02 Revised:2004-02-06 Online:2004-04-26 Published:2020-11-20

摘要: 目的:观察小牛血去蛋白提取物注射液(deproteinized extract of calf blood, DECB) 对培养的心肌细胞缺氧再给氧损伤的保护作用。方法:以培养的心肌细胞为模型, 在缺氧再给氧损伤细胞后, 观察心肌细胞活力及搏动频率的变化, 测定培养液上清乳酸脱氢酶的含量。结果:DECB 0.1 及0.3 g·L-1可以提高损伤后心肌细胞的活力, 提高心肌细胞的搏动频率, 并使培养液上清乳酸脱氢酶的含量明显降低。结论:DECB 对缺氧再给氧损伤培养的心肌细胞具有保护作用。

关键词: 心肌细胞, 培养的, 小牛血去蛋白提取物注射液, 缺氧再给氧损伤

Abstract: AIM: To explore the effects of DECB (deproteinized extract of calf blood, DECB) on hypoxiareoxygenation-induced injury in cultured cardiac myocytes.METHODS: By using cultured myocardial cells as models, thenumber of death cells, beating frequency and the content of LDH were observed after hypoxia-reoxygenation-induced injury. RESULTS: 0.1 and 0.3 g·L-1 of DECB decreased the number of death cells and the content of lactate dehydrogenase (LDH), and restored the cellular beating frequency in the supernatant. CONCLUSION: DECB can protect the cultured myocardial cells against hypoxia-reoxygenation-induced injury.

Key words: myocardial cells, cultured, deproteinized extract of calf blood, hypoxia-reoxygenation-induced injury

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