四种归肝经明目中药防护晶状体氧化损伤和上皮细胞凋亡的研究

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (4): 441-446.

四种归肝经明目中药防护晶状体氧化损伤和上皮细胞凋亡的研究

黄秀榕, 祁明信, 汪朝阳, 王勇

福建中医学院病理生理研究中心, 福州350003, 福建

收稿日期:2003-08-10 修回日期:2003-12-10 出版日期:2004-04-26 发布日期:2020-11-20
通讯作者: 黄秀榕, 女, 教授, 博士生导师, 研究方向:白内障临床与基础研究。Tel Fax:0591-3570887 E-mail:qihuang@netease.com
基金资助:
福建省科技厅重点项目(№98Z173);福建省卫生厅重点项目(№2000W601)

Effects of four Chinese herbs which pass through liver-channel on improving eyesight and protecting oxidative injury of lens and apoptosis of lens epithelial cells

HUANG Xiu-Rong, QI Ming-Xin, WANG Zhao-Yang, WANG Yong

Fujian College of Traditional Chinese Medicine, Fuzhou 350003, Fujian, China

Received:2003-08-10 Revised:2003-12-10 Online:2004-04-26 Published:2020-11-20

摘要/Abstract

摘要： 目的:探讨四种归肝经明目中药车前子、青葙子、菊花和熟地对晶状体氧化损伤及晶状体上皮细胞(lens epithelial cell, LEC) 凋亡的防护作用。方法:将21 只(42 眼) 新西兰白兔晶状体分成7 组:空白对照组、Fenton 组、吡诺克辛钠(PS) 组和车前子、青葙子、菊花及熟地四种归肝明目中药组。采用Fenton 反应复制兔晶状体氧化损伤模型, 在显微镜下观察各组晶状体的混浊程度, 并分别测定晶状体酶性和非酶性抗氧化剂水平。同样, 将21 只(42眼) SD 大鼠双眼随机分成上述7 组, 采用H₂O₂ 复制LEC 凋亡模型, 同时分别采用上述不同药物干预。应用TUNEL 法检测LEC 凋亡及凋亡率、透射电子显微镜观察LEC 超微结构改变和凋亡小体形成。结果:四种归肝明目中药组的晶状体混浊程度均明显轻于Fenton 组;四种归肝明目中药组晶状体中酶性抗氧化剂的活性和非酶性抗氧化剂的含量均明显高于Fenton 组。四种归肝明目中药组LEC 凋亡率均显著低于H₂O₂ 组(P <0.01), 且与PS 组均有显著性差异(P <0.05) 。超微结构研究显示, H₂O₂ 组绝大多数LEC 发生凋亡, 并呈凋亡各期改变;四种归肝明目中药组均仅有少数LEC 发生凋亡, 并多为早期或中期的轻微改变。结论:四种归肝经明目中药均可通过提高晶状体的抗氧化能力对抗晶状体的氧化损伤, 抑制LEC 凋亡, 减轻晶状体的混浊, 且明显优于PS 滴眼液。

关键词: 车前子, 青葙子, 菊花, 熟地, 晶状体, 晶状体上皮细胞, 氧化损伤, 凋亡

Abstract: AIM: To study effects of four Chinese herbs i.e.Semem Plantaginis (SP), Semem Celosiae (SC), Flos Chrysanthemi (FC) and Radix Rehmanniae Preparata (RRP), which pass through liver-channel on improving eyesight and protecting oxidative injury of lens and apoptosis of lens epithelial cell (LEC).METHODS: 21 New Zealand rabbits (42 lenses) were divided randomly into 7 groups:control group, Fenton group, Pirinoxine odium (PS) group and four Chinese herbs (SP, SC, FC and RRP) groups.Lens oxidative injury models were induced by Fenton reaction.Lens opacities were observed via surgical microscope.The contents of superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), total protein (TP), and soluble protein (SP) in all lenses were measured.21 Sprague-Dawley rats (42 lenses) were divided randomly into above 7 groups.Apoptosis of LEC were induced by H₂O₂.The rates of LEC apoptosis in all groups were detected by terminal deoxynucleotidyl transferase mediated biotin-dUTP nick end labeling (TUNEL), and the morphological changes were observed by transmission electron microscope.RESULTS: Lens opacities in groups of four Chinese herbs (SP, SC, FC and RRP) were much lighter than that in Fenton groups.The contents of SOD, GSH, and GSH-Px in lenses of four Chinese herb groups were higher than Fenton groups (P <0.01).The rates of LEC apoptosis in lenses of four Chinese herb groups (16.0 ± 2.8, 30.0 ±2.3, 21.0 ±2.5, 43.1 ±3.5) were significantly lower than that in H₂O₂ (92.0 ±2.55) and PS (56.0 ±9.9) group (P <0.05).The ultrastructure study indicated that the most LEC in H₂O₂ group showed apoptosis and there were in any stages of apoptosis observed by transmission electron microscope.Apoptosis cells in LEC of four Chinese herb groups were much less than that in H₂O₂ group and PS group, and the apoptosis changes of cells were in early or middle stages.CONCLUSION: The four Chinese herbs can obviously increase anti-oxidative ability, protect oxidative damages, alleviate opacities of lenses, and inhibit LEC apoptosis, which are better than that of PS.

Key words: Semem Plantaginis, Semem Closiae, Flos Chrysanthemi, Radix rehmanniae Preparata, oxidative injury, apoptosis, lens, lens epithelial cells

中图分类号:

R285.5

黄秀榕, 祁明信, 汪朝阳, 王勇. 四种归肝经明目中药防护晶状体氧化损伤和上皮细胞凋亡的研究[J]. 中国临床药理学与治疗学, 2004, 9(4): 441-446.

HUANG Xiu-Rong, QI Ming-Xin, WANG Zhao-Yang, WANG Yong. Effects of four Chinese herbs which pass through liver-channel on improving eyesight and protecting oxidative injury of lens and apoptosis of lens epithelial cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(4): 441-446.

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