脱氧核酶抑制耐甲氧西林金黄色葡萄球菌耐药基因的表达

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (6): 641-645.

脱氧核酶抑制耐甲氧西林金黄色葡萄球菌耐药基因的表达

刘杰, 罗晓星, 孟静茹, 扈本荃, 王海芳, 孟嘉

第四军医大学药理学教研室, 西安710032, 陕西

收稿日期:2004-04-02 修回日期:2004-05-08 发布日期:2020-11-22
通讯作者: 罗晓星, 男, 教授, 博士生导师, 研究方向:分子药理、抗生素耐药性治疗。Tel:029-83374591 E-mai l:xxluo3@fmmu.edu.cn
作者简介:刘杰, 男, 硕士研究生, 助教, 研究方向:分子药理、抗生素耐药性治疗。Tel:029-83374556 　E-mai l:liujie25 @fmmu.edu.cn
基金资助:
国家自然科学基金项目(No30271556); 全军医药卫生科研基金项目(No02M008)

DNAzymes inhibit the expression of Methecillin-resistant staphlococcus aureus drug-resistant genes

LIU Jie, LUO Xiao-Xing, MENG Jing-Ru, HU Ben-Quan, WANG Hai-Fang, MENG Jia

Department of Pharmacology, the Fourth Military Medical University, Xian 710032, Shansi, China

Received:2004-04-02 Revised:2004-05-08 Published:2020-11-22

摘要/Abstract

摘要： 目的探讨抑制耐甲氧西林金黄色葡萄球菌(MRSA)耐药基因blaR1 表达的脱氧核酶对MRSA耐药性的影响。方法以耐药基因blaR1 的mRNA为靶点, 设计合成脱氧核酶DrzB, 导入细菌后通过平板克隆形成实验观察DrzB 对MRSA 细菌耐药性的影响, 通过RT-PCR 观察DrzB 对耐药基因blaR1表达的抑制作用。结果加入DrzB 后培养的MRSA,在含苯唑西林(6 mg·L^-1)的M-H 琼脂培养基表面生长的菌落单元计数低于对照组(P <0.01), blaR1的mRNA 表达水平也较对照组降低(P <0.01)。结论导入以blaR1 的mRNA 为靶基因的DrzB 阻断耐药基因表达, 可以部分恢复MRSA 的抗生素敏感性。脱氧核酶DrzB 是一种特异性的、有效的基因治疗剂。

关键词: 耐甲氧西林金黄色葡萄球菌, 耐药性, 基因表达, 脱氧核酶, 抑止效应

Abstract: AIM: To evaluate the inhibition effects of DNAzymes specific to Methecillin-resistant staphylococcus aureus (MRSA)drug-resistant gene blaR1 on the expressions of drug-resistance. METHODS: mRNA of blaR1 was chosen as a target gene and DNAzyme DrzB specific to it was designed and synthesized.After DrzB was introduced into the MRSA, drug-resistant characters of MRSA were evaluated by cloning efficiency.The inhibition effects of DrzB on the expressions of drug-resistant gene blaR1 were observed by RT-PCR in MRSA. RESULTS: Colony forming units (CFU)of MRSA incubated with DrzB on the M-H agar added oxa (6 mg·L^-1)were less than those of control group (P <0.01).Levels of blaR 1 mRNA of the DrzB group were lower than those of the control group (P <0.01). CONCLUSION: Antibiotic sensitivity on MRSA can be partially restored by exogenously delivered DrzB targeted to mRNA of blaR1 in blocking drug-resistant genes expression.DNAzyme DrzB is proved a specific and effective gene therapeutic means.

Key words: Methecillin-resistant staphlococcus aureus(MRSA), drug-resistance, gene expression, DNAzyme, inhibition

中图分类号:

R965.1

刘杰, 罗晓星, 孟静茹, 扈本荃, 王海芳, 孟嘉. 脱氧核酶抑制耐甲氧西林金黄色葡萄球菌耐药基因的表达[J]. 中国临床药理学与治疗学, 2004, 9(6): 641-645.

LIU Jie, LUO Xiao-Xing, MENG Jing-Ru, HU Ben-Quan, WANG Hai-Fang, MENG Jia. DNAzymes inhibit the expression of Methecillin-resistant staphlococcus aureus drug-resistant genes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(6): 641-645.

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