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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (6): 683-686.

• 研究原著 • 上一篇    下一篇

中华眼镜蛇毒F组分对血管平滑肌细胞增殖的影响

余清声, 张梅, 覃媛, 黄劭   

  1. 广州医学院广州蛇毒研究所, 广州510182, 广东
  • 收稿日期:2004-02-03 修回日期:2004-05-19 发布日期:2020-11-22
  • 作者简介:余清声, 男, 教授, 硕士生导师, 研究方向:药理学与蛇毒开发利用。Tel:020-81340322  E-mail:sds yqs @163.net;sds_yqs @126.com
  • 基金资助:
    广东省自然科学基金项目(No980463); 卫生部科技基金课题(No98-2-349)

Effect of fraction F of Naja naja atra venom on proliferation of vascular smooth muscle cells

YU Qing-sheng, ZHANG Mei, QIU Yuan, HANG Shao   

  1. Guangzhou Research Institute of Snake Venom, Guangzhou Medical College, Guangzhou 510182, Guangdong, China
  • Received:2004-02-03 Revised:2004-05-19 Published:2020-11-22

摘要: 目的 研究中华眼镜蛇毒F 组分对血管平滑肌细胞增殖的影响。 方法 用体外培养的血管平滑肌细胞, 观察F 组分对20 %小牛血清引起的细胞对氚标胸腺嘧啶核苷酸(H3-TdR) 掺入的影响, 用蛋白质免疫印迹法(Western blotting) 测定F 组分对20 %小牛血清引起的细胞内原癌基因、核增殖抗原(Proliferationcell nuclear antigen, PCNA) 表达的影响。 结果 F 组分呈浓度依赖性抑制20 %小牛血清引起的细胞对H3-TdR 掺入率和细胞内C-myc、PCNA 的表达, 其中30、100 mg·L-1给药组与对照组比较有显著差异(P <0.05)。 结论 中华眼镜蛇毒F 组分通过影响细胞内原癌基因C-myc、PCNA 的表达, 抑制血管平滑肌细胞的增殖。

关键词: 中华眼镜蛇毒F 组分, 血管平滑肌细胞, 增殖, H3-TdR, C-myc, PCNA, 蛋白质免疫印迹法

Abstract: AIM: To study the effect of fraction F of Naja naja atra venom on proliferation of vascular smooth muscle cells (VSMC). METHODS: The rate of incorporation of [ 3H] thymidine (3H-TdR) into VSMC was observed by cultured VSMC in vitro, and expression of oncogene C-myc and proliferation cell nuclear antigen (PCNA) in VSMC stimulated by 20 % serum was assayed by protein immunoblotting (western blotting). RESULTS: Fration F significantly inhibited the incorporation of3HTdR into VSMCs and the expression of oncogene C-myc and PCNA in VSMC which was stimulated by 20 %serum in a dose-dependent manner.Groups of 30 and 100 mg·L-1 had obvious difference (P <0.05) when compared with control group. CONCLUSION: Fraction F of Naja naja atra venom inhibits the proliferation of VSMC by affecting expression of oncogene C-myc and PCNA.

Key words: Fraction F of Naja naja atra venom, vascular smooth muscle cells, proliferation, H3-TdR, Cmyc, PCNA, Western blotting

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