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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (7): 803-806.

• 研究原著 • 上一篇    下一篇

水飞蓟宾对抗大鼠酒精性脂肪肝作用及机制

谭海荣, 吴谦1, 潘竞锵, 肖柳英, 韩超, 林洁茹2, 郑琳颖2, 李博萍2   

  1. 广州市中医中药研究所, 广州510130, 广东;
    1广东省药物研究所, 广州510180, 广东;
    2广州市中医医院, 广州510130, 广东
  • 收稿日期:2004-04-21 修回日期:2004-06-10 出版日期:2004-07-26 发布日期:2020-11-20
  • 通讯作者: 潘竞锵,男,副主任药师,从事药理实验研究工作,研究方向:抗糖尿病药物和胰岛素增敏剂的药理研究。Tel:020-81953030 E-mail:zysgz@163.com
  • 作者简介:谭海荣,男,副主任医师,从事中西医结合消化、泌尿系统疾病内科和药理实验研究工作。
  • 基金资助:
    广东省中医药局滚动资助项目(№302003);广州市中医药、中西医结合课题资助项目(№200237)

Effects ant mechanism of silibinin on antagonizing alcoholic fatty liver in rats

TAN Hai-Rong, WU Qian1, PAN Jing-Qiang, XIAO Liu-Ying, HAN Chao, LIN Jie-Ru2, ZHENG Lin-Ying2, LI Bo-Ping2   

  1. Guangzhou Institute of Traditional Chinese Medicine and Materia Medica, Guangzhou 510130, Guangdong, China;
    1Guangdong Provincial Institute of Materia Medica, Guangzhou 510180, Guangdong, China;
    2Guangzhou Hospital of Traditional Chinese Medicine, Guangzhou 510130, Guangdong, China
  • Received:2004-04-21 Revised:2004-06-10 Online:2004-07-26 Published:2020-11-20

摘要: 目的: 观察水飞蓟宾(Silbinin)对大鼠酒精性脂肪肝作用及其机制。方法: 饮用10 %乙醇,加高脂高热量饲料喂饲6 周,造成大鼠酒精性脂肪肝模型;灌胃给药水飞蓟宾100mg·kg-1,继续酒精和高脂高热量饲料处理4 周后,处死大鼠,测定血清天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(AKP)活性和甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)、大鼠肿瘤坏死因子-α(TNF-α)、大鼠转化生长因子-β1(TGF-β1)含量,测定肝细胞TG、肝匀浆丙二醛(MDA)、超氧化歧化酶(SOD)、还原性谷胱甘肽(GSH)水平以及肝组织学检查。结果: 水飞蓟宾能抑制病鼠血清AST、ALT、AKP活性(P<0.05或0.01)和降低TG、LDL-C、TNF-α、TGF-β1 含量(P<0.05或0.01),提高HDL-C 含量;降低肝匀浆TG 和MDA 含量(P<0.05或0.01),增强SOD 活性(P<0.01)以及改善肝细胞水变性和脂肪变性(P<0.01)。结论: 水飞蓟素阻止大鼠酒精性脂肪肝形成,其作用机制包括:抗氧化、清除自由基代谢产物,抑制脂质过氧化反应;调血脂、减少脂肪在肝脏的沉积;抗免疫性炎症和抗增殖。

关键词: 水飞蓟宾, 酒精性脂肪肝, 自由基损伤, 肿瘤坏死因子-α, 转化生长因子-β1

Abstract: AIM: To investigate the effects and mechanism of silibinin(Sil)on rats with alcoholic fatty liver.METHODS: The animal models with alcoholic fatty liver were prepared in rats by feeding on high caloric food and drinking 10 % alcohol qd for 10 weeks ;after 6-week treatment, the effects of Sil (intragastrointestinally treated 100 mg·kg-1, qd for 4 weeks)were observed by serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AKP), and serum contents of total cholesterols (TC), triacylglycerol (TG), low-density lipoprotein cholesterol (LDLC), high-density lipoprotein cholesterol (HDL-C), tumor necrosis factor (TNF)-αand transforming growth factor (TGF)-β1, and the contents of TG, malondialdehyde (MDA), glutathione (GSH), and activity of superoxide dismutase (SOD)in the hepatic tissue.Hepatic histopathology was observed by microscope.RESULTS: Serum levels of ALT, AST and AKP decreased in administration of Sil (P<0.05 or 0.01), the levels of TG, LDL-C, TNF-αand TGF-β1 reduced (P<0.05 or 0.01), the concentrations of TG and MDA in the hepatic tissue lowered (P<0.01), and the activity of SOD potentiated (P<0.01).Hydropic and or fatty degeneration of hepatocytes were improved (P<0.01).CONCLUSION: Silibinin can preclude alcoholic fatty liver in rats from drinking alcohol and feeding high caloric food, and pharmacological mechanisms were involved in anti-oxidation and removing free radical, inhibiting lipid peroxidation, adapting blood lipid component, reducing fatty sediment in liver, anti-inflammation and anti-hyperplasia.

Key words: silibinin, ethanolic fatty liver, free radical injure, tumor necrosis factor-α, transforming growth factor-β1

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