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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (1): 69-74.

• 研究原著 • 上一篇    下一篇

三种荧光分光光度法测定肿瘤组织中阿霉素含量的研究

臧凯宏, 张宝来, 王维波, 马建秀, 鞠洋, 高明堂, 李文广, 吴勇杰   

  1. 兰州大学药学院, 甘肃省中药新药临床前研究重点实验室, 兰州 730000, 甘肃
  • 收稿日期:2004-10-15 修回日期:2004-11-28 出版日期:2005-01-26 发布日期:2020-11-19
  • 通讯作者: 高明堂, 男, 教授, 硕士生导师, 研究方向:心血管药理学。Tel:0931-8617780 E-mail:gaomingtang@mail.lazmc.edu.cn
  • 作者简介:臧凯宏, 男, 硕士, 药师, 研究方向:心血管药理学。Tel:0931-3231285 E-mail:zkh2002169@tom.com

Research on three fluorospectrophotometry methods for determination of concentration of adriamycin in tumor tissue

ZANG Kai-hong, ZHANG Bao-lai, WANG Wei-bo, MA Jian-xiou, JU Yang, GAO Ming-tang, LI Wen-guang, WU Yong-jie   

  1. College of Pharmaceutical Science of Lanzhou University, Key Laboratory of Preclinical Study for New Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, Gansu, China
  • Received:2004-10-15 Revised:2004-11-28 Online:2005-01-26 Published:2020-11-19

摘要: 目的: 通过比较测定肿瘤组织中阿霉素(ADR)含量的3 种荧光分光光度法, 建立简便、快捷的测定肿瘤组织中阿霉素含量的荧光分光光法。方法: 分别采用正丁醇法、硝酸银法、酸性异丙醇法测定水溶液及肿瘤组织匀浆中ADR 的含量, 比较荧光光谱曲线、回收率和灵敏度。结果: 硝酸银法测定瘤组织匀浆中的ADR 含量, 背景荧光值低, 线性范围0.2~0.8 mg·L-1, 最低检出量0.15 mg·L-1, 回收率超过97 %以上。酸性异丙醇法与正丁醇法测定瘤组织中的ADR 含量, 背景荧光值高, 回收率分别<40 %和50 %。结论: 硝酸银法适合肿瘤组织中的ADR 的含量测定, 该方法准确、灵敏、可靠。

关键词: 阿霉素, 荧光分光光度法, 肿瘤组织, 含量测定

Abstract: AIM: To establish a simple and rapid fluorospectrophotometry method for the determination of concentration of adriamycin (ADR)in tumor tissue by comparing with three fluorospectrophotometry methods. METHODS: The concentrations of ADR in water and in tumor tissue were measured by three fluorospectrophotometry methods: n-butanol, silver nitrate and acidityisopropanol assay.The fluorescence spectrum curve, recovery and sensitivity were compared.RESULTS: The method of silver nitrate for determination of concentration of ADR in tumor tissue had low background tissue fluorescence.The linear range was 0.2-0.8 mg·L-1, the limit of detection was 0 .15 mg·L-1, and the recovery was over 97% by silver nitrate method .The method of acidity- isopropanol and n-butanol for determination of ADR in tumor-tissue fluorescence and low recovery which was <40 % by acidity-isopropanol assay and <50 % by n-butanol assay, respectively.CONCLUSION: The method of silver nitrate shows accurate, sensitive and reliable characterization for determination of concentration of ADR in tumor tissue.

Key words: adriamycin, fluorospectrophotometry, tumor tissue, assay

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