星形胶质细胞毒蕈碱样乙酰胆碱受体M2、M4亚型基因克隆及序列分析

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (10): 1108-1111.

星形胶质细胞毒蕈碱样乙酰胆碱受体M₂、M₄亚型基因克隆及序列分析

张晓娟, 汪海

军事医学科学院毒物药物研究所, 北京 100850

收稿日期:2005-08-29 修回日期:2005-09-26 发布日期:2020-11-23
通讯作者: 汪海,男,教授,博士导师,从事分子心血管药理学及新药研究。Tel:010-66932651 E-mail:wh@nic.hmi.ac.cn
作者简介:张晓娟,女,硕士研究生,从事分子心血管药理学研究。Tel:01066931557 Email:zhangxj904@yahoo.com.cn
基金资助:
国家自然科学基金项目(No30371641);军事医学科学院创新基金资助

Sequence analysis of muscarinic receptors M₂, and M₄ expression by cultured rat astrocyte cells

ZHANG Xiao-juan, WANG Hai

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100580, China

Received:2005-08-29 Revised:2005-09-26 Published:2020-11-23

摘要/Abstract

摘要： 目的: 克隆胶质细胞M₂、M₄受体亚型基因序列,并比较版质细胞M₂、M₄受体亚型基因序列和蛋白质序列与神经元细胞M₂、M₄受体基因序列和蛋白质序列间的差异。方法: 根据神经元细胞M₂、M₄受体基因序列设计出针对M₂、M₄受体基因序列全长的特异性探针,采用RT-PCR方法扩增胶质细胞M₂、M₄受体亚型基因序列,并对其进行克隆测序。结果: 通过RT-PCR方法扩增胶质细胞M₂、M₄受体亚型基因序列,与神经元细胞M₂、M₄受体比较,M₂受体差异碱基17个,发生氨基酸改变的有8个;M₄ 受体差异碱基3个,发生氨基酸改变的有2个。结论: 胶质细胞M₂、M₄受体与神经细胞M₂、M₄受体亚型在基因序列和氨基酸序列上具有明显差异。

关键词: 非神经性胆碱系统, 星形胶质细胞, 基因克隆, 聚合酶链式反应, 序列分析, M受体

Abstract: AIM:To clone the muscarinic receptor M₂, M₄ sequence of astrocyte cell, and compare the geneand protein sequence with those of neuron.METHODS: The specific primers were designed to clone the M₂ and M₄, sequence of astrocyte cellng to the sequences that of neuron by RT-PCR, and then the sequences were sequenced. RESHLTS AND CONCLUSIONS: The M₂ and M₄ sequences expression were compared by astrocyte cells and neurons, and the difference was marked. The differences of the gene and protein sequences are evidentof M₂ and M₄ between astrocyte cell and neuron.

Key words: non-neuronal cholinergic system: astro-cyte cell, gene clone, PCR, sequence analysis, muscarunic receptor

中图分类号:

R965.1

张晓娟, 汪海. 星形胶质细胞毒蕈碱样乙酰胆碱受体M₂、M₄亚型基因克隆及序列分析[J]. 中国临床药理学与治疗学, 2005, 10(10): 1108-1111.

ZHANG Xiao-juan, WANG Hai. Sequence analysis of muscarinic receptors M₂, and M₄ expression by cultured rat astrocyte cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(10): 1108-1111.

参考文献

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星形胶质细胞毒蕈碱样乙酰胆碱受体M₂、M₄亚型基因克隆及序列分析

Sequence analysis of muscarinic receptors M₂, and M₄ expression by cultured rat astrocyte cells

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