幼年家兔口服苯妥英锌 7 个月小脑生长发育的研究

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (11): 1294-1298.

幼年家兔口服苯妥英锌 7 个月小脑生长发育的研究

蒲其松, 杨健全¹, 雷军, 李春平², 兰大荣¹, 张翔, 吴体成¹, 蔡春燕

川北医学院药物研究所,南充 637000,四川;
¹巴中市人民医院,巴中 675000,四川;
²川北医学院附属医院 CT 室,南充 637000,四川

收稿日期:2005-09-15 修回日期:2005-10-13 出版日期:2005-11-26 发布日期:2020-11-12
基金资助:
四川省教育厅自然科学基金资助课题(No01LA31)

Development of cerebellum with 7 months orally phenytoin zinc in child-hood rabbits

PU Qi-song, YANG Jian-quan¹, LEI Jun, LI Chun-ping², LAN Da-rong¹, ZHANG Xiang, WU Ti-cheng¹, CAI Chun-yan

Institute of Materia Medica,North Sichuan Medical College,Nanchong 63700,Sichuan,China;
¹Department of Phar-macy,Bazhong People' s Hospital,Bazhong 675000,Sichuan,China;
²Department of CT,Affiliated Hospital of North Sichuan Medical College,Nanchong 63700,Sichuan,China

Received:2005-09-15 Revised:2005-10-13 Online:2005-11-26 Published:2020-11-12

摘要/Abstract

摘要： 目的: 考察长期口服苯妥英锌(phenytoinzinc,PZ)对幼年家兔小脑生长发育的影响。方法: 1月龄新西兰家兔分别给予PZ或苯妥英钠(phenytoinsodi-um,PS)7个月(剂量分别为:30、10mg·kg^-1·d^-1),药前、药中(4个月)和药后进行三次CT扫描,测量小脑最大横径和蚓部最大横径。服药结束后处死动物,Bouin氏液灌注固定脑组织,测定全脑的长、宽和高以及重量。结果: CT扫描显示PS组服药4个月和7个月后,小脑最大横径分别缩短14.0%(P<0.05)和32.2%(P<0.01);蚓部最大横径分别缩短16.4%(P<0.05)和30.8%(P<0.01)。PZ组仅在服药7个月后,小脑和蚓部的最大横径比对照组分别缩短16.1%(P<0.05)和19.7%(P<0.05)。固定后的小脑和蚓部的长度和宽度,PS组均比对照组明显缩短;全脑湿重和乙醇脱水后重量以及小脑重量与对照组比较均显著减轻,其中全脑脱水后重量减轻15.4%(P<0.05),小脑重量减轻18.4%(P<0.05)。而PZ组的上述测定值与对照组均无显著性差异。结论: 幼兔长期口服高剂量PS后导致小脑萎缩,而口服PZ后对小脑的形态影响比PS轻,有可能降低本类抗癫痫药物长期服用后引起的小脑萎缩。

关键词: 苯妥英锌, 苯妥英钠, CT, 幼年兔, 小脑

Abstract: AIM: To investigate developing cerebel-lum with long-term orally phenytoin zinc(PZ)orpheny-toin sodium(PS)in the childhood rabbits.METHODS: One-month-old young rabbits were taken PZ orPS orally for7 months(doses:30 and 10 mg·kg^-1·d^-1).The rab-bits were performed forCT scans before,during and afteradministrated.Taken drugs end,brains were fixed with Brouin' s pourfrom carotid,separated and determined weight and width,length and height of the brain.RESULTS: In the high-dose grouPtreated with PS,at the 4 and 7 months crosscut of cerebellum were decreased by 14.0%(P<0.05)and 32.2%(P<0.01)and crosscut of vermis were decreased by 16.4%(P<0.05)and 30.8%(P<0.01)respectively compared with the control with CT scan analysis.In PZ high-dose grouPonly at 7months,crosscut of cerebellum and vermis were decreased by 16.1%(P<0.05)and 19.7%(P<0.01),respectively compared with the control by CT.In PS high-dose grouPthe width and length of fixed cere-bellum and vermis were more short than control,the wet weight and dehydrated weight with ethanol of the whole brain were decreased than control,the weight of whole brain and cerebellum was decreased by 15.4% and 18.4%respectively compared with control.Nevertheless in PZ grouPabove-mentioned determine were no differ-ence with control.CONCLUSION: Long-term use of PS may cause cerebellaratrophy in child rabbit,while PZ may lowerthe cerebellaratrophy.

Key words: phenytoin zinc, phenytoin sodium, CT scans, cerebellum, childhood rabbit

中图分类号:

R971

蒲其松, 杨健全, 雷军, 李春平, 兰大荣, 张翔, 吴体成, 蔡春燕. 幼年家兔口服苯妥英锌 7 个月小脑生长发育的研究[J]. 中国临床药理学与治疗学, 2005, 10(11): 1294-1298.

PU Qi-song, YANG Jian-quan, LEI Jun, LI Chun-ping, LAN Da-rong, ZHANG Xiang, WU Ti-cheng, CAI Chun-yan. Development of cerebellum with 7 months orally phenytoin zinc in child-hood rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(11): 1294-1298.

[1]	鲁琼, 朱海宏, 戚听听, 李国华, 滕欣琪, 屈强, 屈健. OCTN2基因多态性影响结直肠癌奥沙利铂化疗敏感性[J]. 中国临床药理学与治疗学, 2021, 26(4): 401-407.
[2]	谢彦, 李澄, 张露露, 俞飞, 臧士明, 付晶晶, 刘璐, 王峰. ⁶⁸Ga-PSMA-I&T与初诊前列腺癌病灶结合的研究[J]. 中国临床药理学与治疗学, 2021, 26(12): 1379-1385.
[3]	韩贵娟, 江骥, 欧阳伟炜, 罗鸿, 张晓军, 王明华. 放射性同位素示踪法研究CT-707在健康男性受试者体内的吸收和排泄[J]. 中国临床药理学与治疗学, 2021, 26(12): 1407-1412.
[4]	张明清，刘玲娟，田杰. EGCG升高cTnIR193H限制型心肌病模型小鼠未突变cTnI的表达水平[J]. 中国临床药理学与治疗学, 2019, 24(4): 376-382.
[5]	赵江涛，梁延鸽. 葛花总黄酮对异丙肾上腺素致大鼠内耳损伤后相关炎性因子影响的实验研究[J]. 中国临床药理学与治疗学, 2018, 23(9): 1003-1007.
[6]	吕思敏，黄志荣，于琼，孙金影，崔燎，吴铁. 辅酶Q10及其伴侣饮料对轻度衰老期小鼠股骨和尺骨的影响[J]. 中国临床药理学与治疗学, 2018, 23(3): 290-295.
[7]	叶海主，陈亚娟，赵文英，刘晓平. 肿瘤细胞和耐药肿瘤细胞中FoxO3/Keap1/Nrf2通路的表达差异[J]. 中国临床药理学与治疗学, 2017, 22(8): 859-865.
[8]	陈瑛，梁海燕，李海军. 人参皂苷Rg1对lactacystin诱导SH-SY5Y细胞损伤的保护作用[J]. 中国临床药理学与治疗学, 2016, 21(7): 754-758.
[9]	刘英姿，曾飞跃，肖乐东，王果. 焦磷酸测序分析CTR1基因多态性方法的建立[J]. 中国临床药理学与治疗学, 2016, 21(6): 673-677.
[10]	许标波，贺毅憬，王韦力，周成芳，谢商宸，沈冬亚，Howard L. Mcleod. 肿瘤免疫检查点抑制剂临床治疗的研究进展[J]. 中国临床药理学与治疗学, 2016, 21(2): 218-224.
[11]	况赟, 孙雪, 阳喜定, 龚丽英, 郭成贤, 李静乐, 阳国平. 华法林定量药理学的研究进展[J]. 中国临床药理学与治疗学, 2015, 20(7): 814-821.
[12]	季娜, 张根葆, 黄璐, 王斐, 张娅, 吴娟. 蝮蛇毒血小板抑制因子对家兔动脉血栓形成的影响[J]. 中国临床药理学与治疗学, 2014, 19(9): 987-990.
[13]	黄小燕, 郭歆, 余鹏, 刘智, 黄志军, 阳国平, 程泽能. 磷苯妥英钠注射液人体药代动力学研究[J]. 中国临床药理学与治疗学, 2014, 19(4): 419-423.
[14]	蔡珂, 周睿, 时百玲. Oct-4基因在胃癌顺铂耐药中的作用[J]. 中国临床药理学与治疗学, 2012, 17(3): 251-255.
[15]	刘红红, 陈小华, 周丽芹, 刘雪妮, 余永胜, 臧国庆, 汤正好. CTP-HBcAg18-27-Tapasin融合蛋白胞内转导功能的检测[J]. 中国临床药理学与治疗学, 2012, 17(1): 25-29.