左旋精氨酸和维生素C合用对自发性高血压大鼠左室肥厚及血管内皮功能的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (12): 1384-1388.

左旋精氨酸和维生素C合用对自发性高血压大鼠左室肥厚及血管内皮功能的影响

张平洋, 邓又斌¹, 杨好意¹, 毕小军¹, 潘敏¹

南京医科大学附属南京第一医院心血管超声科, 南京 210000, 江苏;
¹华中科技大学同济医学院附属同济医院, 武汉 430000 湖北

收稿日期:2005-10-11 修回日期:2005-11-25 出版日期:2005-12-26 发布日期:2020-11-11

Effect of L-Arginine and vitamin C on left ventricularhypertrophy and endothelial function in spontaneously hypertensive rats

ZHANG Ping-yang, DENG You-bin¹, YANG Hao-yi¹, BI Xiao-jun¹, PAN Min¹

Department of CardialvascularUltrasonography,First Hospital of Nanjing Affiliated to Nanjing Medical University,Nanjing 210000,J iangsu,China;
¹Department of Medical Ultrasound,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,Hubei,China

Received:2005-10-11 Revised:2005-11-25 Online:2005-12-26 Published:2020-11-11

摘要/Abstract

摘要： 目的: 探讨自发性高血压大鼠左室肥厚与内皮功能改变间的关系以及药物改善内皮功能、抑制左室肥厚的可能性。方法: 20 只8 周龄的SHr随机分成实验组(12 只) 和治疗组(8 只),以8 只同龄WKY 作正常对照组。给予治疗组含左旋精氨酸(浓度为7.5 g·L^-1) 和维生素C(浓度为4 ~ 5 g·L^-1) 的饮水持续12周,实验组和正常对照组给予蒸馏水饮用。20 周龄的大鼠测血压后处死,常规测量心脏重量、左室重量,生化检测大鼠血浆一氧化氮水平应用HE 、VG 染色,结合计算机图像分析技术,检测心肌细胞直径、心肌组织胶原分数、血管周围胶原分数。结果: (1) 与正常对照组相比,实验组SHr的血压增高、心率增快,左室重量指数(LVMI) 显著增高,血浆一氧化氮(NO) 浓度显著减小,心肌细胞直径(TDM)增大、心肌胶原分数(CVF) 、血管周围胶原分数(PCA) 均显著增高。两组间体重无显著性差异(P>0.05) 。相关分析表明:实验组SHr的LVMI 与血浆NO 呈负相关(r=-0.53,P<0.05),而与TDM 、CVF 、PCA 呈正关(r=0.80 、0.68 、0.66,P<0.01),血浆NO 与TDM 、CVF 、PCA 呈负相关(r=-0.65 、-0.54 、-0.52,P<0.01 或0.05) 。(2) 与实验组相比,治疗组SHr的LVMI 、TDM 、CVF 、PCA 均减低,血浆NO 水平增高,两组间的血压、心率、体重均无显著性差异(P>0.05) 。与正常对照相比,治疗组血压、心率、LVMI 、TDM 、CVF 、PCA 显著增高,而体重、血浆NO 水平无显著性差异(P>0.05) 。结论: SHR内皮功能障碍影响心肌细胞肥大及心肌纤维化的过程是其参与影响LVH 的形成的主要环节,药物改善内皮功能可以在一定程度上阻遏这一过程发展。

关键词: 自发性高血压, 左室肥厚, 血管内皮功能, 左旋精氨酸, 维生素C

Abstract: AIM: To explore the relationshiPbetween left ventricularhypertrophy (LVH) and endothelial function(EF) and to investigate the effects of L-Arginine and Vitamin C on LVH and EF in spontaneously hypertensive rats (SHR).METHODS: 20 SHrwith 8 weeks old were randomly divided into experiment grouP(12 rats)and treated grouP(8 rats) while 8 WKY rats were selected as a control group.The SHrof treated grouPreceived L-Arginine and Vitamin C (7.5 and 4-5 g·L^-1 respectively in drinking water) for12 weeks,but the WKY rats and the SHrof experiment grouPwere given distilled wateras drinking water.The rats with 20 weeks old were killed aftermeasurements of theirblood pressure and body weight.HE and VG staining combined with computed morphometry were employed to evaluate the cardiomyocyte diameter(CDM),the collagen volume fraction (CVF),perivascularcircumferential area (PCA) on the left ventriculartissue.RESULTS: Compared with the WKY,the blood pressure was higher,heart rate was faster,left ventricularmass (LVMI) were higher,NO was lowerin the experimental group.The CVF,PCA and CDM were higherin the experimental group.There were no differences in body weight between the experiment grouPand the control.Bivariate analysis showed that in the experiment grouPLVMI correlated well with NO,CVF,PCA orCDM (r=0.80,0.68,0.66,P<0.01).Besides,NOin the experiment grouPcorrelated well with CDM,CVF orPCA (r=-0.65,-0.54,-0.52,P<0.01 orP<0.05).Compared with the experiment group,LVMI,CDM,CVF,and PCA were lowerin the treated group.There was higherNO level in the treated group.There were no significant differences in blood pressure,body weight and heart rate.Compared with the control,blood pressure,heart rate,LVMI,CDM,CVF and PCA still increased.There were no significant difference in body weight and in NO between WKY and the treated group.CONCLUSIONS: LVH is related with endothelial dysfunction in SHR.The endothelial dysfunction may play a role in the development and progression of LVH by accelerating CDM and CVF and PCA increased.LL-Arginine and Vitamin C can hinderoff LVH to some degree by improving endothelial function.

Key words: spontaneously hypertensive, left ventricularypertrophy, endothelial function, L-arginine, vitamin C

中图分类号:

R965.1

张平洋, 邓又斌, 杨好意, 毕小军, 潘敏. 左旋精氨酸和维生素C合用对自发性高血压大鼠左室肥厚及血管内皮功能的影响[J]. 中国临床药理学与治疗学, 2005, 10(12): 1384-1388.

ZHANG Ping-yang, DENG You-bin, YANG Hao-yi, BI Xiao-jun, PAN Min. Effect of L-Arginine and vitamin C on left ventricularhypertrophy and endothelial function in spontaneously hypertensive rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(12): 1384-1388.

参考文献

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